Citation: Nan Li, Weifei Zhang, Haifeng Lin, Jin-Ming Lin. Di-4-ANEPPDHQ probes the response of lipid packing to the membrane tension change in living cells[J]. Chinese Chemical Letters, ;2022, 33(3): 1377-1380. doi: 10.1016/j.cclet.2021.08.060 shu

Di-4-ANEPPDHQ probes the response of lipid packing to the membrane tension change in living cells

Nan Li ^a ,
Weifei Zhang ^{b, *,} ,
Haifeng Lin ^a ,
Jin-Ming Lin ^{a, *,}

a.
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
b.
Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China

zhangweifei@nim.ac.cn

jmlin@mail.tsinghua.edu.cn

Received Date: 25 May 2021
Revised Date: 26 June 2021
Accepted Date: 9 August 2021
Available Online: 16 August 2021

Figures(4)

Membrane tension plays a significant role in many cellular processes including cell adhesion, migration and spreading. Despite the importance of membrane tension, it remains difficult to measure in vivo. Recently, the development of non-invasive fluorescent probes have made great progress, especially excited-state deplanarization in molecular rotors has been applied to image membrane tension in living cells. Nevertheless, an intrinsic limitation of such kind of probe is that they depend on the lipid packing, and how the lipid packing responds to the membrane tension change remains unclear. Therefore, in this work, we used a polarity-sensitive membrane probe to investigate the possible response mechanism of lipid packing to the change of membrane tension that was regulated by osmotic shocks. The results showed that an increase in membrane tension could stretch the lipids apart with large displacements, and this change was not homogeneous on the whole membrane, instead, increase of membrane tension induced phase separation.
- Lipid packing,
- Membrane tension,
- Osmotic shocks,
- Polarity-sensitive probe,
- Phase separation
1. [1]
  B. Pontes, P. Monzo, L. Gole, et al., J. Cell Biol. 216(2017) 2959-2977. doi: 10.1083/jcb.201611117
2. [2]
  S. Mao, W. Zhang, Q. Huang, et al., Angew. Chem. Int. Ed. 57(2018) 236-240. doi: 10.1002/anie.201710273
3. [3]
  K. Tsujita, T. Takenawa, T. Itoh, Nat. Cell Biol. 17(2015) 749-758. doi: 10.1038/ncb3162
4. [4]
  D. Zhang, F. Feng, R. Liu, W. Zhu, L. Yao, Chin. Chem. Lett. 30(2019) 7-14. doi: 10.1016/j.cclet.2018.06.011
5. [5]
  B. Lin, X. Lu, N. Li, N. Xu, J.M. Lin, Chin. Chem. Lett. 31(2020) 476-478. doi: 10.1016/j.cclet.2019.07.066
6. [6]
  N.C. Gauthier, M.A. Fardin, P. Roca-Cusachs, M.P. Sheetz, Proc. Natl. Acad. Sci. U. S. A. 108(2011) 14467. doi: 10.1073/pnas.1105845108
7. [7]
  A.R. Houk, A. Jilkine, C.O. Mejean, et al., Cell 148(2012) 175-188. doi: 10.1016/j.cell.2011.10.050
8. [8]
  A.D. Lieber, S. Yehudai-Resheff, E.L. Barnhart, J.A. Theriot, K. Keren, Curr. Biol. 23(2013) 1409-1417. doi: 10.1016/j.cub.2013.05.063
9. [9]
  M. Saleem, S. Morlot, A. Hohendahl, et al., Nat. Commun. 6(2015) 6249. doi: 10.1038/ncomms7249
10. [10]
  C. Gabella, E. Bertseva, C. Bottier, et al., Curr. Biol. 24(2014) 1126-1132. doi: 10.1016/j.cub.2014.03.050
11. [11]
  A.S. Klymchenko, Acc. Chem. Res. 50(2017) 366-375. doi: 10.1021/acs.accounts.6b00517
12. [12]
  J. Gong, P. Wei, Y. Su, et al., Chin. Chem. Lett. 29(2018) 1493-1496. doi: 10.1016/j.cclet.2018.05.043
13. [13]
  M.A. Haidekker, E.A. Theodorakis, J. Mater. Chem. C 4(2016) 2707-2718. doi: 10.1039/C5TC03504J
14. [14]
  A. Colom, E. Derivery, S. Soleimanpour, et al., Nat. Chem. 10(2018) 1118-1125. doi: 10.1038/s41557-018-0127-3
15. [15]
  T. Harayama, H. Riezman, Nat. Rev. Mol. Cell Biol. 19(2018) 281-296. doi: 10.1038/nrm.2017.138
16. [16]
  M. Mackley, Rheol. Acta 49(2010) 443-458. doi: 10.1007/s00397-010-0435-z
17. [17]
  A. Suhaj, D. Gowland, N. Bonini, D.M. Owen, C.D. Lorenz, J. Phys. Chem. B 124(2020) 11419-11430. doi: 10.1021/acs.jpcb.0c09496
18. [18]
  L. Jin, A.C. Millard, J.P. Wuskell, et al., Biophys. J. 90(2006) 2563-2575. doi: 10.1529/biophysj.105.072884
19. [19]
  D.M. Owen, S. Oddos, S. Kumar, et al., Mol. Membr. Biol. 27(2010) 178-189. doi: 10.3109/09687688.2010.495353
20. [20]
  D.M. Owen, C. Rentero, A. Magenau, A. Abu-Siniyeh, K. Gaus, Nat. Protoc. 7(2012) 24-35. doi: 10.1038/nprot.2011.419
21. [21]
  J.L. Sampaio, M.J. Gerl, C. Klose, et al., Proc. Natl. Acad. Sci. U. S. A. 108(2011) 1903. doi: 10.1073/pnas.1019267108
22. [22]
  J.C.S. Ho, P. Rangamani, B. Liedberg, A.N. Parikh, Langmuir 32(2016) 2151-2163. doi: 10.1021/acs.langmuir.5b04470
23. [23]
  T. Hamada, Y. Kishimoto, T. Nagasaki, M. Takagi, Soft Matter 7(2011) 9061-9068. doi: 10.1039/c1sm05948c
1. [1]
  Ruotong Wei , Aokun Liu , Jian Kuang , Zhiwen Wang , Lu Yu , Changlin Tian . Probing the dynamic properties in the LLPS process via site-directed spin labeling-electron paramagnetic resonance (SDSL-EPR) spectroscopy. Chinese Chemical Letters, 2025, 36(4): 110029-. doi: 10.1016/j.cclet.2024.110029
2. [2]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
3. [3]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020
4. [4]
  Xu Qu , Baohua Ji , Haocheng Gong , Guangwei Wang , Liang-Liang Gao , Jing Zhang , Jianjian Zhang , Yuan Guo . Dual-emissive near-infrared fluorogenic probe with enhanced cellular uptake capability for sensitive tracking of cellular polarity. Chinese Chemical Letters, 2025, 36(10): 110766-. doi: 10.1016/j.cclet.2024.110766
5. [5]
  Shan-Qing Yang , Lu-Lu Wang , Rajamani Krishna , Bo Xing , Lei Zhou , Fei-Yang Zhang , Qiang Zhang , Yi-Long Li , Chao-Sheng Bao , Tong-Liang Hu . Efficient C₃H₆/C₃H₈ separation within a bifunctional ultramicroporous metal-organic framework with high purity and record packing density. Chinese Chemical Letters, 2025, 36(12): 110556-. doi: 10.1016/j.cclet.2024.110556
6. [6]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
7. [7]
  Quan Lu , Lulu Zhang , Zihan Chen , Jiajia Lv , Jie Gao , Xinmin Li , Hongyu Li , Wen Shi , Xiaohua Li , Huimin Ma , Zeli Yuan . A lipid droplet-targeting fluorescence probe for monitoring of lipid peroxidation in ferroptosis and non-alcoholic fatty liver disease. Chinese Chemical Letters, 2025, 36(8): 110620-. doi: 10.1016/j.cclet.2024.110620
8. [8]
  Yan Zou , Yin-Shuang Hu , Deng-Hui Tian , Hong Wu , Xiaoshu Lv , Guangming Jiang , Yu-Xi Huang . Tuning the membrane rejection behavior by surface wettability engineering for an effective water-in-oil emulsion separation. Chinese Chemical Letters, 2024, 35(6): 109090-. doi: 10.1016/j.cclet.2023.109090
9. [9]
  Changle Liu , Mingyuzhi Sun , Haoran Zhang , Xiqian Cao , Yuqing Li , Yingtang Zhou . All in one doubly pillared MXene membrane for excellent oil/water separation, pollutant removal, and anti-fouling performance. Chinese Journal of Structural Chemistry, 2024, 43(8): 100355-100355. doi: 10.1016/j.cjsc.2024.100355
10. [10]
  Zhoupeng Zheng , Shengyi Gong , Qianhua Li , Shiya Zhang , Guoqiang Feng . Lipid droplets and gallbladder targeted fluorescence probe for ratiometric NO imaging in gallstones disease models. Chinese Chemical Letters, 2025, 36(5): 110191-. doi: 10.1016/j.cclet.2024.110191
11. [11]
  Yukai Tong , Zhijun Wu , Bo Zhou , Min Hu , Anpei Ye . Surface tension of single suspended aerosol microdroplets. Chinese Chemical Letters, 2024, 35(4): 109062-. doi: 10.1016/j.cclet.2023.109062
12. [12]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820
13. [13]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
14. [14]
  Shuangying Li , Qingxiang Zhou , Zhi Li , Menghua Liu , Yanhui Li . Sensitive measurement of silver ions in environmental water samples integrating magnetic ion-imprinted solid phase extraction and carbon dot fluorescent sensor. Chinese Chemical Letters, 2024, 35(5): 108693-. doi: 10.1016/j.cclet.2023.108693
15. [15]
  Guilong Li , Wenbo Ma , Jialing Zhou , Caiqin Wu , Chenling Yao , Huan Zeng , Jian Wang . A composite hydrogel with porous and homogeneous structure for efficient osmotic energy conversion. Chinese Chemical Letters, 2025, 36(2): 110449-. doi: 10.1016/j.cclet.2024.110449
16. [16]
  Yujie Xie , Kexin Yuan , Beiyang Luo , Haoran Feng , Xian Bao , Jun Ma . Osmotic membranes for municipal wastewater reclamation: Insights into applications, transmembrane diffusion mechanisms and prospects. Chinese Chemical Letters, 2025, 36(7): 110443-. doi: 10.1016/j.cclet.2024.110443
17. [17]
  Jialin Guo , Mingrui Gu , Yahui Chen , Tao Xiong , Yiyang Zhang , Simin Chen , Mingle Li , Xiaoqiang Chen , Xiaojun Peng . Nucleic acid delivery by lipid nanoparticles for organ targeting. Chinese Chemical Letters, 2025, 36(11): 110849-. doi: 10.1016/j.cclet.2025.110849
18. [18]
  Linfang Wang , Jing Liu , Minghao Ren , Wei Guo . A highly sensitive fluorescent HClO probe for discrimination between cancerous and normal cells/tissues. Chinese Chemical Letters, 2024, 35(6): 108945-. doi: 10.1016/j.cclet.2023.108945
19. [19]
  Lanyun Zhang , Weisi Wang , Yu-Qiang Zhao , Rui Huang , Yuxun Lu , Ying Chen , Liping Duan , Ying Zhou . Mechanism study of the molluscicide candidate PBQ on Pomacea canaliculata using a viscosity-sensitive fluorescent probe. Chinese Chemical Letters, 2025, 36(1): 109798-. doi: 10.1016/j.cclet.2024.109798
20. [20]
  Bangdi GE , Xiaowei SONG , Zhiqiang LIANG . A bifunctional three-dimensional Eu-MOF fluorescent probe for highly sensitive detection of 2, 4, 6-trinitrophenol and tetracycline. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 2165-2174. doi: 10.11862/CJIC.20250190

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(1773)
HTML views(227)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142