Citation: Yongqing Zhou, Zhenzhen Liu, Guangming Qiao, Bo Tang, Ping Li. Visualization of endoplasmic reticulum viscosity in the liver of mice with nonalcoholic fatty liver disease by a near-infrared fluorescence probe[J]. Chinese Chemical Letters, ;2021, 32(11): 3641-3645. doi: 10.1016/j.cclet.2021.04.035 shu

Visualization of endoplasmic reticulum viscosity in the liver of mice with nonalcoholic fatty liver disease by a near-infrared fluorescence probe

Yongqing Zhou ^a ,
Zhenzhen Liu ^a ,
Guangming Qiao ^b ,
Bo Tang ^a ,
Ping Li ^{a, *,}

a.
College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, China
b.
Environment Pollution Control Center, Zibo 255000, China

lip@sdnu.edu.cn

Received Date: 19 February 2021
Revised Date: 8 April 2021
Accepted Date: 18 April 2021
Available Online: 27 April 2021

Figures(6)

Nonalcoholic fatty liver disease (NAFLD) can cause serious liver damage. Early diagnosis and effective treatment of NAFLD can greatly improve treatment rates. The initiation and development of NAFLD has been closely linked to endoplasmic reticulum (ER) stress, which might cause ER viscosity variations. Therefore, if the internal relationship between ER viscosity and NAFLD is clarified, an effective approach for early diagnosis may result. Herein, we fabricated a novel near-infrared (NIR) fluorescence imaging probe, Er-V, for monitoring ER viscosity through a molecular rotor strategy. Er-V exhibited a strong NIR fluorescence signal (at 626 nm) when the environmental viscosity hindered the rotation of the malononitrile group. Using Er-V, we successfully observed a significant enhancement in viscosity in the liver of mice with NAFLD. Therefore, this imaging method based on Er-V is expected to provide a new approach for early detection and diagnosis of NAFLD.
- Nonalcoholic fatty liver disease,
- Endoplasmic reticulum stress,
- Viscosity,
- Fluorescence imaging probe
1. [1]
  L.A. Adams, P. Angulo, K.D. Lindor, CMAJ 172 (2015) 899-905
2. [2]
  M. Zobair, M.D. Younossi, Clin. Liver Dis. 11 (2018) 92-94 doi: 10.1002/cld.710
3. [3]
  L. Vigano, A. Lleo, A. Aghemo, HepatoBiliary Surg. Nutr. 7 (2018) 130-133 doi: 10.21037/hbsn.2018.01.01
4. [4]
  A. Sircana, E. Paschetta, F. Saba, et al., Int. J. Mol. Sci. 20 (2019) 1745 doi: 10.3390/ijms20071745
5. [5]
  E. Cleveland, A. Bandy, L.B. WanWagner, Clin. Liver Dis. 11 (2018) 98-104 doi: 10.1002/cld.716
6. [6]
  Y. Sumida, A. Nakajima, Y. Itoh, J. Gastroenterol. 20 (2014) 475-485
7. [7]
  J.D. Browning, J.D. Horton, J. Clin. Investig. 114 (2004) 147-152 doi: 10.1172/JCI200422422
8. [8]
  H. Nakagawa, A. Umemura, K. Taniguchi, et al., Cancer Cell 26 (2014) 1-13 doi: 10.1016/j.ccr.2014.06.015
9. [9]
  J.Y. Kim, R.G. Carbonell, S. Yamachika, et al., Cell 175 (2018) 133-145 doi: 10.1016/j.cell.2018.08.020
10. [10]
  P. Puri, F. Maridoddin, O. Cheung, et al., Gastroenterology. 134 (2008) 568-576 doi: 10.1053/j.gastro.2007.10.039
11. [11]
  M. Masarone, V. Rosato, M. Dallio, et al., Oxid. Med. Cell Longev. 2018 (2018) 9547613
12. [12]
  R. Guo, J.L. Yin, Y.Y. Ma, et al., Sens. Actuators B Chem. 271 (2018) 321-328 doi: 10.1016/j.snb.2018.05.055
13. [13]
  X.J. Peng, Z.H. Zhang, J.Y. Wang, et al., J. Am. Chem. Soc. 133 (2011) 6626-6635 doi: 10.1021/ja1104014
14. [14]
  K. Dou, W.J. Huang, Y.H. Xiang, et al., Anal. Chem. 92 (2020) 4177-4181 doi: 10.1021/acs.analchem.0c00634
15. [15]
  Z.G. Yang, Y.X. He, J.H. Lee, et al., Chem. Commun. 50 (2014) 11672-11675 doi: 10.1039/C4CC04915B
16. [16]
  Y.Q. Zhou, P. Li, X. Wang, et al., Chem. Sci. 11 (2020) 12149-12156 doi: 10.1039/d0sc02922j
17. [17]
  S.X. Zhou, B.C. Zhou, N. Wang, et al., Chin. Chem. Lett. 31 (2020) 2897-2902 doi: 10.1016/j.cclet.2020.03.037
18. [18]
  X.F. Zhang, L. Wang, N. L, et al., Chin. Chem. Lett. 32 (2021) 2395-2399. doi: 10.1016/j.cclet.2021.02.031
19. [19]
  X.D. Wang, L. Fan, S.H. Wang, et al., Anal. Chem. 93 (2020) 3241-3249
20. [20]
  C.X. Chen, H.B. Fang, X.T. Shao, et al., Nat. Commun. 11 (2020) 6290 doi: 10.1002/chem.202001486
21. [21]
  X.B. Xiao, P. Li, X.F. Hu, et al., Chem. Sci. 7 (2016) 6153-6159 doi: 10.1039/C6SC01793B
22. [22]
  W. Chen, J.Y. Han, J.X. She, et al., Chem. Common. 56 (2020) 7797-7800 doi: 10.1039/d0cc00868k
23. [23]
  Y. He, J.W. Shin, W.J. Gong, et al., Chem. Commun. 55 (2019) 2453-2456 doi: 10.1039/c9cc00300b
24. [24]
  B.C. Chen, C.D. Li, J. Zhang, Chem. Commun. 55 (2019) 7410-7413 doi: 10.1039/c9cc03977e
25. [25]
  X. Wang, P. Li, Q. Ding, et al., Angew. Chem. Int. Ed. 58 (2019) 4674-46788 doi: 10.3390/en12244674
26. [26]
  H.B. Xiao, C.C. Wu, P. Li, Chem. Sci. 8 (2017) 7025-7030 doi: 10.1039/C7SC02330H
27. [27]
  J.F. Ji, T. Homma, S. Kobayashi, et al., World J. Biol. Chem. 9 (2018) 1-15 doi: 10.1097/CEJ.0000000000000420
28. [28]
  J. Hu, W. Hong, K.N. Yao, et al., J. Gastroenterol. 25 (2019) 1492-1501
29. [29]
  X.J. Peng, Z.G. Yang, J.Y. Wang, et al., J. Am. Chem. Soc. 133 (2011) 6626-6635 doi: 10.1021/ja1104014
30. [30]
  T. Karlas, R. Physician, J. Wiegand, et al., J. Clin. Endocrinol. Metab. 27 (2013) 195-208
31. [31]
  M.C. Heffern, H.M. Park, H.Y. Au-Yeung, et al., Proc. Natl. Acad. Sci. U. S. A. 113(2016) 14219-14224 doi: 10.1073/pnas.1613628113
32. [32]
  D. Cheng, X.Y. Gong, Q. Wu, et al., Anal. Chem. 92 (2020) 11396-11404 doi: 10.1021/acs.analchem.0c02277
33. [33]
  Y. Chang, S. Ryu, E. Sung, et al., Clin. Chem. 53 (2007) 686-692 doi: 10.1373/clinchem.2006.081257
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9. [9]
  Qiuye Wang , Yabing Sun , Liangxue Lai , Haijing Cui , Yonglong Ye , Ming Yang , Weihao Zhu , Bo Yuan , Quanliang Mao , Wenzhi Ren , Aiguo Wu . MMP-9-responsive probe for fluorescence-magnetic resonance dual-mode imaging of hepatocellular carcinoma models with different metastatic capacities. Chinese Chemical Letters, 2025, 36(4): 110212-. doi: 10.1016/j.cclet.2024.110212
10. [10]
  Linfang ZHANG , Wenzhu YIN , Gui YIN . A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 540-548. doi: 10.11862/CJIC.20240405
11. [11]
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