Citation: Wenqian Liu, Yulin Xu, Xue Li, Yan Meng, Huiling Wang, Chunrong Liu, Changlin Liu, Li Wang. A DNA G-quadruplex converts SOD1 into fibrillar aggregates[J]. Chinese Chemical Letters, ;2021, 32(7): 2322-2326. doi: 10.1016/j.cclet.2021.01.045 shu

A DNA G-quadruplex converts SOD1 into fibrillar aggregates

Wenqian Liu ^{a, 1} ,
Yulin Xu ^{a, 1} ,
Xue Li ^{a, b} ,
Yan Meng ^a ,
Huiling Wang ^a ,
Chunrong Liu ^a ,
Changlin Liu ^{a, *,} ,
Li Wang ^{a, *,}

a.
Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, and School of Chemistry, Central China Normal University, Wuhan 430079, China
b.
Wuhan No. 1 Huiquan Middle School, Wuhan 430022, China

liuchl@mail.ccnu.edu.cn

wl_928@mail.ccnu.edu.cn

Received Date: 12 November 2020
Revised Date: 4 January 2021
Accepted Date: 26 January 2021
Available Online: 28 January 2021

Figures(5)

Nucleic acids with G4 elements play a role in the formation of aggregates involved in intracellular phase transitions. Our previous studies suggest that different forms of DNA could act as an accelerating template in Cu/Zn superoxide dismutase (SOD1) aggregation. Here, we examined the regulation of formation and cytotoxicity of the SOD1 aggregates by single-stranded 12-mer deoxynucleotide oligomers (dN)₁₂ (N = A, T, G, C; ssDNAs) under acidic conditions. The ssDNAs can be divided into two groups based on their roles in SOD1 binding, exposure of hydrophobic clusters in SOD1, accelerated formation, morphology and cytotoxicity of SOD1 aggregates. G-quadruplexes convert SOD1 into fibrillar aggregates as a template, a fact which was observed for the first time in the nucleic acid regulation of protein aggregation. Moreover, the fibrillar or fibril-like SOD1 species with a G-quadruplex provided by (dG)₁₂ were less toxic than the amorphous species with (dN)₁₂ (N = A, T). This study not only indicates that both morphology and cytotoxicity of protein aggregates can be regulated by the protein-bound DNAs, but also help us understand roles of nucleic aid G-quadruplexes in the formation of aggregates and membraneless organelles involved in intracellular phase transitions.
- SOD1,
- Deoxynucleotide oligomers (dN)₁₂,
- G-quadruplexes,
- Fibrillar aggregates
1. [1]
  M. Calamai, J.R. Kumita, J. Mifsud, et al., Biochem. 45 (2006) 12806-12815. doi: 10.1021/bi0610653
2. [2]
  J.D. Domizio, R. Zhang, L.J. Stagg, et al., J. Biol. Chem. 287 (2012) 736-747. doi: 10.1074/jbc.M111.238477
3. [3]
  C.L. Liu, Y. Zhang, Adv. Protein Chem. Struct. Biol. 84 (2011) 1-40. doi: 10.1145/2037715.2037759
4. [4]
  J.D. Domizio, S. Dorta-Estremera, M. Gagea, et al., Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 14550 ̵ 14555. doi: 10.1073/pnas.1206923109
5. [5]
  C.N. Wong, L.W. Xiong, M. Horiuchi, et al., EMBO J. 20 (2001) 377-386. doi: 10.1093/emboj/20.3.377
6. [6]
  B. Macedo, T.A. Millen, C.A.C.A. Braga, et al., Biochem. 51 (2012) 5402-5413. doi: 10.1021/bi300440e
7. [7]
  S.F. Banani, A.M. Rice, W.B. Peeples, et al., Cell 166 (2016) 651-663. doi: 10.1016/j.cell.2016.06.010
8. [8]
  A. Khong, T. Matheny, S. Jain, et al., Cell 68 (2017) 808-820. doi: 10.1016/j.molcel.2017.10.015
9. [9]
  P. Ivanov, E. O'Day, M.M. Emara, et al., Proc. Natl. Acad. Sci. U. S. A. 111 (2014) 18201-18206. doi: 10.1073/pnas.1407361111
10. [10]
  Y. Zhang, C. Roland, C. Sagui, Neurosci. 9 (2018) 1104-1117. doi: 10.1021/acschemneuro.7b00476
11. [11]
  X. Li, J.Y. Shi, S. Qiu, et al., Prog. Chem. 30 (2018) 1475-1486.
12. [12]
  X. Li, Y. Chen, J. Zhao, et al., Oxid. Med. Cell. Longev. 2019 (2019) 9706792.
13. [13]
  B. J. Carter, P. Anklesaria, S. Choi, et al., Antioxid. Redox Sign. 11 (2009) 1569-1586. doi: 10.1089/ars.2008.2414
14. [14]
  A. E. Frakes, L. Ferraiuolo, A. M. Haidet-Phillips, et al., Neuron 81 (2014) 1009-1023. doi: 10.1016/j.neuron.2014.01.013
15. [15]
  X. Li, S. Qiu, J. Shi, et al., Nucleic Acids Res. 47 (2019) 5074-5085. doi: 10.1093/nar/gkz256
16. [16]
  C.K. Tsang, M. Chen, X. Cheng, et al., Mol. Cell 70 (2018) 502-515. doi: 10.1016/j.molcel.2018.03.029
17. [17]
  A.R. Reddi, V.C. Culotta, Cell 152 (2013) 224-235. doi: 10.1016/j.cell.2012.11.046
18. [18]
  W. Jiang, Y.C. Han, R.Y. Zhou, et al., Biochem. 46 (2007) 5911-5923. doi: 10.1021/bi062234m
19. [19]
  W. Jiang, B. Zhang, J. Yin, et al., Biopolymers 89 (2008) 1154-1169. doi: 10.1002/bip.21067
20. [20]
  J. Yin, S. Hu, W. Jiang, et al., PLoS One 5 (2010) e12328. doi: 10.1371/journal.pone.0012328
21. [21]
  J. Yin, R.Z. Chen, C.L. Liu, Front. Biosci. 14 (2009) 5084-5106. doi: 10.2741/3588
22. [22]
  Y. Yue, F. Huo, P. Yue, et al., Anal. Chem. 90 (2018) 7018-7024. doi: 10.1021/acs.analchem.8b01406
23. [23]
  D. S. Leeman, K, Hebestreit, T. Ruetz, et al., Science 359 (2018) 1277-1283. doi: 10.1126/science.aag3048
24. [24]
  G. Habicht, C. Haupt, R.P. Friedrich, et al., Proc. Natl. Acad. Sci. U. S. A. 104 (2007) 19232-19237. doi: 10.1073/pnas.0703793104
25. [25]
  G.C.P. van Zundert, J.P.G.L.M. Rodrigues, M. Trellet, et al., J. Mol. Biol. 428 (2015) 720-725. doi: 10.1016/j.jmb.2015.09.014
26. [26]
  D. Zhao, X. Dong, N. Jiang, et al., Nucleic Acids Res. 42 (2014) 11612-11622. doi: 10.1093/nar/gku833
27. [27]
  S. Stefansson, D.L. Adams, C.M. Tang, et al., BioTechniques 52 (2012) 1-6. doi: 10.1155/2012/371487
28. [28]
  J. Choi, S. Kim, T. Tachikawa, et al., J. Am. Chem. Soc. 133 (2011) 16146-16153. doi: 10.1021/ja2061984
29. [29]
  A. Rajendran, S.I. Nakano, N. Sugimoto, Chem. Commun. 46 (2010) 1299-1301 doi: 10.1039/b922050j
30. [30]
  S. Pramanik, S. Nagatoishi, N. Sugimoto, Chem. Commun. 48 (2012) 4815-4817. doi: 10.1039/c2cc30622k
31. [31]
  R. Jin, B.L. Gaffney, C. Wang, et al., Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 8832-8836. doi: 10.1073/pnas.89.18.8832
32. [32]
  A. Rajendran, B.U. Nair. BBA-Gen, Subjects 1760 (2006) 1794-1801. doi: 10.1016/j.bbagen.2006.08.011
33. [33]
  C.M. Karch, M. Prudencio, D.D. Winkler, et al., Proc. Natl. Acad. Sci. U. S. A. 106 (2009) 7774-7779. doi: 10.1073/pnas.0902505106
34. [34]
  M. Chattopadhyay, A. Durazo, S.H. Sohn, et al., Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 18663-18668. doi: 10.1073/pnas.0807058105
35. [35]
  Z.A.O. Durer, J.A. Cohlberg, P. Dinh, et al., PLoS One 4 (2009) e5004. doi: 10.1371/journal.pone.0005004
36. [36]
  J.S. Elam, A.B. Taylor, R. Strange, et al., Nat. Struct. Mol. Biol. 10 (2003) 461-467. doi: 10.1038/nsb935
37. [37]
  M. Bucciantini, E. Giannoni, F. Chiti, et al., Nature 416 (2002) 507-511. doi: 10.1038/416507a
38. [38]
  I. Choi, Y.I. Yang, H.D. Song, et al., Biochim. Biophys. Acta 1812 (2011) 41-48. doi: 10.1016/j.bbadis.2010.09.003
39. [39]
  S.J. Weisberg, R. Lyakhovetsky, A.C. Werdiger, et al., Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 15811-15816. doi: 10.1073/pnas.1205829109
40. [40]
  G.W. Collie, R. Promontorio, S.M. Hampel, et al., J. Am. Chem. Soc. 134 (2012) 2723-2731. doi: 10.1021/ja2102423
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