Citation: Mei Liu, Lei Xi, Ting Tan, Lian Jin, Zhifei Wang, Nongyue He. A novel aptamer-based histochemistry assay for specific diagnosis of clinical breast cancer tissues[J]. Chinese Chemical Letters, ;2021, 32(5): 1726-1730. doi: 10.1016/j.cclet.2020.11.072 shu

A novel aptamer-based histochemistry assay for specific diagnosis of clinical breast cancer tissues

Mei Liu ^{a, b, 1} ,
Lei Xi ^{c, 1} ,
Ting Tan ^{d, 1} ,
Lian Jin ^{a, e} ,
Zhifei Wang ^{b, **} ,
Nongyue He ^{a, e, *}

a.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education (Southeast University), School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
c.
Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
d.
Department of Pathology, Hunan Provincial Maternal and Child Health Care Hospital, Changsha 410008, China
e.
Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China

zfwang@seu.edu.cn

nyhe1958@163.com

Received Date: 27 September 2020
Revised Date: 27 November 2020
Accepted Date: 4 December 2020
Available Online: 4 December 2020

Figures(4)

Pathological detection using immunohistochemistry (IHC) has become an indispensable process in the diagnosis confirmation of various cancers. However, the production of monoclonal antibodies is always very complex, expensive and time-consuming, and the batch differences are significant due to the corporeity and health statuses of animals may be different. In this work, an aptamer-based histochemistry (aptahistochemistry) assay was developed using a DNA aptamer for specific diagnosis of clinical breast cancer tissue sections. This aptahistochemistry assay can specifically distinguish Luminal A breast cancer molecular subtype from Luminal B (HER2+), HER2-enriched, and triple-negative breast cancer molecular subtypes, as well as para-carcinoma tissue, mastitis tissue and normal breast tissue. The accuracy of this aptahistochemistry assay for the diagnosis of Luminal A breast cancer was as high as 80%, which showed a great potential for clinical pathological diagnosis applications.
- Aptamer,
- Immunohistochemistry,
- Breast cancer,
- Molecular subtype
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