Citation: Ling Li, Xuqi Xue, Huige Zhang, Wenjuan Lv, Shengda Qi, Hongying Du, Anne Manyande, Hongli Chen. In-situ and one-step preparation of protein film in capillary column for open tubular capillary electrochromatography enantioseparation[J]. Chinese Chemical Letters, ;2021, 32(7): 2139-2142. doi: 10.1016/j.cclet.2020.11.022 shu

In-situ and one-step preparation of protein film in capillary column for open tubular capillary electrochromatography enantioseparation

Ling Li ^a ,
Xuqi Xue ^a ,
Huige Zhang ^a ,
Wenjuan Lv ^a ,
Shengda Qi ^a ,
Hongying Du ^{b, *,} ,
Anne Manyande ^c ,
Hongli Chen ^{a, *,}

a.
State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China
b.
Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430071, China
c.
School of Human and Social Sciences, University of West London, Middlesex TW89GA, United Kingdom

hydu@mail.hzau.edu.cn

hlchen@lzu.edu.cn

Received Date: 9 July 2020
Revised Date: 21 September 2020
Accepted Date: 10 November 2020
Available Online: 10 November 2020

Figures(4)

In this work, the phase-transitioned BSA (PTB) film using the mild and fast fabrication process adhered to the capillary inner wall uniformly, and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography (OT-CEC) enantioseparation. The enantioseparation ability of PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters, all achieving good resolution and symmetrical peak shape. For three consecutive runs, the relative standard deviations (RSD) of migration time for intra-day, inter-day, and column-to-column repeatability were in the range of 0.3%–3.5%, 0.2%–4.9% and 2.1%–7.7%, respectively. Moreover, the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency. Therefore, the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.
- Open tubular capillary electrochromatography,
- Proteinaceous phase-transitioned film,
- Bovine serum albumin (BSA),
- Enantioseparation
1. [1]
  J. Shen, Y. Okamoto, Chem. Rev. 116 (2016) 1094-1138. doi: 10.1021/acs.chemrev.5b00317
2. [2]
  J.M. Saz, M.L. Marina, J. Chromatogr. A 1467 (2016) 79-94. doi: 10.1016/j.chroma.2016.08.029
3. [3]
  D. Wu, F. Pan, W. Tan, L. Gao, Y. Tao, Y. Kong, J. Sep. Sci. 42 (2019) 1-11.
4. [4]
  M. Maisuradze, G. Sheklashvili, A. Chokheli, et al., J. Chromatogr. A 1602 (2019) 228-236. doi: 10.1016/j.chroma.2019.05.026
5. [5]
  X. Yao, H. Zheng, Y. Zhang, X. Ma, Y. Xiao, Y. Wang, Anal. Chem. 88 (2016) 4955-4964. doi: 10.1021/acs.analchem.6b00897
6. [6]
  L. Fumagalli, L. Pucciarini, L. Regazzoni, et al., J. Sep. Sci. 41 (2018) 1240-1246. doi: 10.1002/jssc.201701308
7. [7]
  X. Han, J. Huang, C. Yuan, Y. Liu, Y. Cui, J. Am. Chem. Soc. 140 (2018) 892-895. doi: 10.1021/jacs.7b12110
8. [8]
  M. Zhou, Y. Long, Y. Zhi, X. Xu, Chin. Chem. Lett. 29 (2018) 1399-1403. doi: 10.1016/j.cclet.2017.10.039
9. [9]
  B. Zhao, L. Li, Y. Wang, Z. Zhou, Chin. Chem. Lett. 30 (2019) 643-649. doi: 10.1016/j.cclet.2018.10.013
10. [10]
  X. Shi, Y. Zhou, F. Liu, J. Mao, Y. Zhang, T. Shan, J. Chromatogr. A 1596 (2019) 161-174. doi: 10.1016/j.chroma.2019.02.063
11. [11]
  J. Zhang, S. Xie, L. Chen, et al., Anal. Chem. 87 (2015) 7817-7824. doi: 10.1021/acs.analchem.5b01512
12. [12]
  S. Xie, J. Zhang, N. Fu, et al., Anal. Chim. Acta 903 (2016) 156-163. doi: 10.1016/j.aca.2015.11.030
13. [13]
  Y. Sun, C. Li, X. Niu, et al., Talanta 195 (2019) 190-196. doi: 10.1016/j.talanta.2018.11.035
14. [14]
  H. Guo, Y. Sun, X. Niu, et al., J. Chromatogr. A 1578 (2018) 91-98. doi: 10.1016/j.chroma.2018.10.007
15. [15]
  C. Pan, W. Lv, X. Niu, et al., J. Chromatogr. A 1541 (2018) 31-38. doi: 10.1016/j.chroma.2018.02.015
16. [16]
  R. Meng, J. Kang, J. Chromatogr. A 1506 (2017) 120-127. doi: 10.1016/j.chroma.2017.05.010
17. [17]
  L. Zhao, J. Qiao, H. Zhang, F. Xie, L. Qi, Chin. Chem. Lett. 30 (2019) 349-352. doi: 10.1016/j.cclet.2018.03.007
18. [18]
  H. Guo, X. Niu, C. Pan, et al., J. Sep. Sci. 40 (2017) 2645-2653. doi: 10.1002/jssc.201700152
19. [19]
  R. Liang, X. Wang, C. Liu, X. Meng, J. Qiu, J. Chromatogr. A 1323 (2014) 135-142. doi: 10.1016/j.chroma.2013.11.048
20. [20]
  T. Hong, Y. Zheng, W. Hu, Y. Ji, Anal. Biochem. 464 (2014) 43-50. doi: 10.1016/j.ab.2014.07.015
21. [21]
  J. Lu, F. Ye, A. Zhang, et al., J. Sep. Sci. 34 (2011) 2329-2336.
22. [22]
  M. Kato, H. Saruwatari, K. Sakai-Kato, T. Toyo'oka, J. Chromatogr. A 1044 (2004) 267-270. doi: 10.1016/j.chroma.2004.04.056
23. [23]
  M. Kato, K. Sakai-Kato, N. Matsumoto, T. Toyo'oka, Anal. Chem. 74 (2002) 1915-1921. doi: 10.1021/ac0112162
24. [24]
  P. Yang, Macromol. Biosci. 12 (2012) 1053-1059. doi: 10.1002/mabi.201200092
25. [25]
  D.H. Wang, Y. Ha, J. Gu, et al., Adv. Mater. 28 (2016) 7414-7423. doi: 10.1002/adma.201506476
26. [26]
  C. Li, L. Xu, Y. Zuo, P. Yang, Biomater. Sci. 6 (2018) 836-841. doi: 10.1039/C8BM00066B
27. [27]
  X. Hu, J. Tian, C. Li, et al., Adv. Mater. 32 (2020) 2000128. doi: 10.1002/adma.202000128
28. [28]
  C. Liu, R. Liang, X. Wang, J. Wang, J. Qiu, J. Chromatogr. A 1294 (2013) 145-151. doi: 10.1016/j.chroma.2013.04.022
29. [29]
  C. Li, L. Xu, Y.Y. Zuo, P. Yang, Biomater. Sci. 6 (2018) 836-841. doi: 10.1039/C8BM00066B
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