Citation: Xiaolian Li, Jiazhu Zheng, Wenjuan Liu, Qinglong Qiao, Jie Chen, Wei Zhou, Zhaochao Xu. Long-term super-resolution imaging of mitochondrial dynamics[J]. Chinese Chemical Letters, ;2020, 31(11): 2937-2940. doi: 10.1016/j.cclet.2020.05.043 shu

Long-term super-resolution imaging of mitochondrial dynamics

Xiaolian Li ^{a, 1} ,
Jiazhu Zheng ^{a, 1} ,
Wenjuan Liu ^{b, 1} ,
Qinglong Qiao ^{b, *,} ,
Jie Chen ^b ,
Wei Zhou ^b ,
Zhaochao Xu ^{a, b, *,}

a.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
b.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

qqlqiao@dicp.ac.cn

zcxu@dicp.ac.cn

Received Date: 7 May 2020
Revised Date: 26 May 2020
Accepted Date: 30 May 2020
Available Online: 2 June 2020

Figures(4)

Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy. However, long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential. Here, we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives. Using structured illumination microscopy (SIM), we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution. Furthermore, the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale. Convincingly, the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.
- Mitochondria,
- Dynamic,
- Super-resolution,
- Fluorescent imaging,
- SNAP-tag
1. [1]
  J. Bereiter-Hahn, M. Vöth, Microsc. Res. Tech. 27(1994) 198-219. doi: 10.1002/jemt.1070270303
2. [2]
  B. Westermann, W. Neupert, Yeast 16(2000) 1421-1427. doi: 10.1002/1097-0061(200011)16:15<1421::AID-YEA624>3.0.CO;2-U
3. [3]
  H.M. McBride, M. Neuspiel, S. Wasiak, Curr. Biol. 16(2006) R551-R560. doi: 10.1016/j.cub.2006.06.054
4. [4]
  L.C. Gomes, G.D. Benedetto, L. Scorrano, Nat. Cell Biol. 13(2011) 589-598. doi: 10.1038/ncb2220
5. [5]
  S. Hoppins, L. Lackner, J. Nunnari, Annu. Rev. Biochem. 76(2007) 751-780. doi: 10.1146/annurev.biochem.76.071905.090048
6. [6]
  D.C. Chan, Cell 125(2006) 1241-1252. doi: 10.1016/j.cell.2006.06.010
7. [7]
  I.R. Boldogh, L.A. Pon, Trends Cell Biol. 17(2007) 502-510. doi: 10.1016/j.tcb.2007.07.008
8. [8]
  M. Audano, A. Schneider, N. Mitro, J. Neurochem. 147(2018) 291-309. doi: 10.1111/jnc.14471
9. [9]
  Y.C. Wong, S. Kim, W. Peng, D. Krainc, Trends Cell Biol. 29(2019) 500-513. doi: 10.1016/j.tcb.2019.02.004
10. [10]
  S.W. Hell, Science 316(2007) 1153-1158. doi: 10.1126/science.1137395
11. [11]
  B. Huang, H. Babcock, X. Zhuang, Cell 143(2010) 1047-1058. doi: 10.1016/j.cell.2010.12.002
12. [12]
  Q. Hu, C. Qin, L. Huang, H. Wang, Q. Liu, L. Zeng, et al., Dyes Pigments 149(2018) 253-260. doi: 10.1016/j.dyepig.2017.10.002
13. [13]
  J. Xu, J. Pan, X. Jiang, et al., Biosens. Bioelectron. 77(2016) 725-732. doi: 10.1016/j.bios.2015.10.049
14. [14]
  F. Deng, Z. Xu, Chin. Chem. Lett. 30(2019) 1667-1681. doi: 10.1016/j.cclet.2018.12.012
15. [15]
  S. Long, Q. Qiao, L. Miao, Z. Xu, Chin. Chem. Lett. 30(2019) 573-576. doi: 10.1016/j.cclet.2018.11.031
16. [16]
  F. Huang, T.M.P. Hartwich, F.E. Rivera-Molina, et al., Nat. Methods 10(2013) 653-658. doi: 10.1038/nmeth.2488
17. [17]
  L. Schermelleh, P.M. Carlton, S. Haase, et al., Science 320(2008) 1332-1336. doi: 10.1126/science.1156947
18. [18]
  X. Huang, J. Fan, L. Li, et al., Nat. Biotechnol. 36(2018) 451-459. doi: 10.1038/nbt.4115
19. [19]
  M.J. Rust, M. Bates, X. Zhuang, Nat. Methods 3(2006) 793-796. doi: 10.1038/nmeth929
20. [20]
  H. Takakura, Y. Zhang, R.S. Erdmann, et al., Nat. Biotechnol. 35(2017) 773-780. doi: 10.1038/nbt.3876
21. [21]
  Y. Han, M. Li, F. Qiu, M. Zhang, Y.H. Zhang, Nat. Commun. 8(2017) 1307. doi: 10.1038/s41467-017-01503-6
22. [22]
  Q. Chen, X. Shao, Z. Tian, et al., Nano Res. 12(2019) 1009-1015. doi: 10.1007/s12274-019-2331-x
23. [23]
  R.A.J. Smith, R.C. Hartley, M.P. Murphy, Antioxid. Redox Signal. 15(2011) 3021-3038. doi: 10.1089/ars.2011.3969
24. [24]
  J. Zielonka, J. Joseph, A. Sikora, et al., Chem. Rev. 117(2017) 10043-10120. doi: 10.1021/acs.chemrev.7b00042
25. [25]
  S. Long, W. Chi, L. Miao, et al., Chin. Chem. Lett. 30(2019) 601-604. doi: 10.1016/j.cclet.2018.12.008
26. [26]
  S. Leng, Q. Qiao, L. Miao, et al., Chem. Commun. 53(2017) 6448-6451. doi: 10.1039/C7CC01483J
27. [27]
  Q. Qiao, W. Liu, J. Chen, et al., Dyes Pigments 147(2017) 327-333. doi: 10.1016/j.dyepig.2017.08.032
1. [1]
  Jiayu Zeng , Minhui Liu , Ting Yang , Jia Huang , Songjiao Li , Wanting Zhang , Dan Cheng , Longwei He , Jia Zhou . Two-dimensional design strategy to construct smart dual-responsive fluorescent probe for the precise tracking of ischemic stroke. Chinese Chemical Letters, 2025, 36(5): 110166-. doi: 10.1016/j.cclet.2024.110166
2. [2]
  Kun-Heng Li , Hong-Yang Zhao , Dan-Dan Wang , Ming-Hui Qi , Zi-Jian Xu , Jia-Mi Li , Zhi-Li Zhang , Shi-Wen Huang . Mitochondria-targeted nano-AIEgens as a powerful inducer for evoking immunogenic cell death. Chinese Chemical Letters, 2024, 35(5): 108882-. doi: 10.1016/j.cclet.2023.108882
3. [3]
  Xinyi Zhao , Yuai Duan , Zihan Liu , Hua Geng , Yaping Li , Zhongfeng Li , Tianyu Han . Mapping sweat pores for biometric identification based on a donor-acceptor hydrophilic fluorescent probe. Chinese Chemical Letters, 2025, 36(8): 110617-. doi: 10.1016/j.cclet.2024.110617
4. [4]
  Kuan Deng , Fei Yang , Zhi-Qi Cheng , Bi-Wen Ren , Hua Liu , Jiao Chen , Meng-Yao She , Le Yu , Xiao-Gang Liu , Hai-Tao Feng , Jian-Li Li . Construction of wavelength-tunable DSE quinoline salt derivatives by regulating the hybridization form of the nitrogen atom and intramolecular torsion angle. Chinese Chemical Letters, 2024, 35(10): 109464-. doi: 10.1016/j.cclet.2023.109464
5. [5]
  Aoxuan Song , Qinglong Qiao , Ning Xu , Yiyan Ruan , Wenhao Jia , Xiang Wang , Zhaochao Xu . Super-resolution imaging of cellular pseudopodia dynamics with a target-specific blinkogenic probe. Chinese Chemical Letters, 2025, 36(8): 110643-. doi: 10.1016/j.cclet.2024.110643
6. [6]
  Fan Zheng , Runsha Xiao , Shuai Huang , Zhikang Chen , Chen Lai , Anyao Bi , Heying Yao , Xueping Feng , Zihua Chen , Wenbin Zeng . Accurate visualization colorectal cancer by monitoring viscosity variations with a novel mitochondria-targeted fluorescent probe. Chinese Chemical Letters, 2025, 36(2): 109876-. doi: 10.1016/j.cclet.2024.109876
7. [7]
  Tao Yu , Vadim A. Soloshonok , Zhekai Xiao , Hong Liu , Jiang Wang . Probing the dynamic thermodynamic resolution and biological activity of Cu(Ⅱ) and Pd(Ⅱ) complexes with Schiff base ligand derived from proline. Chinese Chemical Letters, 2024, 35(4): 108901-. doi: 10.1016/j.cclet.2023.108901
8. [8]
  Meng Shan , Yongmei Yu , Mengli Sun , Shuping Yang , Mengqi Wang , Bo Zhu , Junbiao Chang . Bifunctional organocatalyst-catalyzed dynamic kinetic resolution of hemiketals for synthesis of chiral ketals via hydrogen bonding control. Chinese Chemical Letters, 2025, 36(1): 109781-. doi: 10.1016/j.cclet.2024.109781
9. [9]
  Pei Huang , Weijie Zhang , Junping Wang , Fangjun Huo , Caixia Yin . Rapid and specific fluorescent probe visualizes dynamic correlation of Cys and HClO in OGD/R. Chinese Chemical Letters, 2025, 36(1): 109778-. doi: 10.1016/j.cclet.2024.109778
10. [10]
  Junliang Zhou , Tian-Bing Ren , Lin Yuan . The strategy to improve the brightness of organic small-molecule fluorescent dyes for imaging. Chinese Chemical Letters, 2025, 36(8): 110644-. doi: 10.1016/j.cclet.2024.110644
11. [11]
  Qian Pang , Fangjun Huo , Yongkang Yue , Caixia Yin . ONOO⁻ and viscosity dual-response fluorescent probe for arthritis imaging in vivo. Chinese Chemical Letters, 2025, 36(9): 110713-. doi: 10.1016/j.cclet.2024.110713
12. [12]
  Zhiwen Li , Jingjing Zhang , Gao Li . Dynamic assembly of chiral golden knots. Chinese Journal of Structural Chemistry, 2024, 43(7): 100300-100300. doi: 10.1016/j.cjsc.2024.100300
13. [13]
  Bin Fang , Jiaqi Yang , Limin Wang , Haoqin Li , Jiaying Guo , Jiaxin Zhang , Qingyuan Guo , Bo Peng , Kedi Liu , Miaomiao Xi , Hua Bai , Li Fu , Lin Li . A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury. Chinese Chemical Letters, 2024, 35(6): 108913-. doi: 10.1016/j.cclet.2023.108913
14. [14]
  Zhiyu Yu , Xiang Luo , Cheng Zhang , Xin Lu , Xiaohui Li , Pan Liao , Zhongqiu Liu , Rong Zhang , Shengtao Wang , Zhiqiang Yu , Guochao Liao . Mitochondria-targeted carrier-free nanoparticles based on dihydroartemisinin against hepatocellular carcinoma. Chinese Chemical Letters, 2024, 35(10): 109519-. doi: 10.1016/j.cclet.2024.109519
15. [15]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020
16. [16]
  Baoli Yin , Xinlin Liu , Zhe Li , Zhifei Ye , Youjuan Wang , Xia Yin , Sulai Liu , Guosheng Song , Shuangyan Huan , Xiao-Bing Zhang . Ratiometric NIR-Ⅱ fluorescent organic nanoprobe for imaging and monitoring tumor-activated photodynamic therapy. Chinese Chemical Letters, 2025, 36(5): 110119-. doi: 10.1016/j.cclet.2024.110119
17. [17]
  Meng Gao , Jiqiu Yin , XianChao Jia , Ye Gao , Yang Jiao . On-target site enriching fluorescent bioprobe for imaging of receptor tyrosine kinase in tumor. Chinese Chemical Letters, 2025, 36(6): 110297-. doi: 10.1016/j.cclet.2024.110297
18. [18]
  Xianghan Zhang , Yuan Qin , Huaicong Zhang , Yutian Cao , Haixing Zhu , Yingdi Tang , Zimeng Ma , Zehua Li , Jialin Zhou , Qunyan Dong , Peng Yang , Yuqiong Xia , Zhongliang Wang . An aggregation-independent and rotor-specific TPE-cyanine probe for in vivo near-infrared fluorescent imaging. Chinese Chemical Letters, 2025, 36(9): 110715-. doi: 10.1016/j.cclet.2024.110715
19. [19]
  Pei Cao , Yilan Wang , Lejian Yu , Miao Wang , Liming Zhao , Xu Hou . Dynamic asymmetric mechanical responsive carbon nanotube fiber for ionic logic gate. Chinese Chemical Letters, 2024, 35(6): 109421-. doi: 10.1016/j.cclet.2023.109421
20. [20]
  Guoliang Liu , Zhiqiang Liu , Anmin Zheng . Modulation of zeolite surface realizes dynamic copper species redispersion. Chinese Journal of Structural Chemistry, 2024, 43(6): 100308-100308. doi: 10.1016/j.cjsc.2024.100308

Get Citation

PDF

XML

Metrics

PDF Downloads(13)
Abstract views(1292)
HTML views(62)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142