Citation: Xiaolian Li, Jiazhu Zheng, Wenjuan Liu, Qinglong Qiao, Jie Chen, Wei Zhou, Zhaochao Xu. Long-term super-resolution imaging of mitochondrial dynamics[J]. Chinese Chemical Letters, ;2020, 31(11): 2937-2940. doi: 10.1016/j.cclet.2020.05.043 shu

Long-term super-resolution imaging of mitochondrial dynamics

Xiaolian Li ^{a, 1} ,
Jiazhu Zheng ^{a, 1} ,
Wenjuan Liu ^{b, 1} ,
Qinglong Qiao ^{b, *,} ,
Jie Chen ^b ,
Wei Zhou ^b ,
Zhaochao Xu ^{a, b, *,}

a.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
b.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

qqlqiao@dicp.ac.cn

zcxu@dicp.ac.cn

Received Date: 7 May 2020
Revised Date: 26 May 2020
Accepted Date: 26 May 2020
Available Online: 15 November 2020

Figures(4)

Monitoring dynamics of mitochondria has become an essential approach to explore the function of mitochondria in living cells with the emergence of super-resolution fluorescence microscopy. However, long-term super-resolution imaging of mitochondria is still challenging due to the lack of photostable fluorescent probes and stable mitochondria-specific markers which are not affected by the changes of mitochondrial membrane potential. Here, we introduce a method for long-term imaging mitochondrial dynamic through the SNAP-tag fluorogenic probe based on 4-azetidinyl-naphthalimide derivatives. Using structured illumination microscopy (SIM), we observed the fusion and fission of mitochondria over a course of 16 min at 109 nm resolution. Furthermore, the interactions as well as fusion between mitochondria and lysosomes were studied during mitophagy at the nanoscale. Convincingly, the combination of SNAP-tag fluorogenic probes and super-resolution fluorescence microscopy will offer a new way to monitor dynamic mitochondria in living cells.
- Mitochondria,
- Dynamic,
- Super-resolution,
- Fluorescent imaging,
- SNAP-tag
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