Citation: Yuyu Wang, Houna Duan, Hongyuan Shi, Shiwei Zhang, Yufang Xu, Weiping Zhu, Xuhong Qian. A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice[J]. Chinese Chemical Letters, ;2020, 31(11): 2933-2936. doi: 10.1016/j.cclet.2020.05.028 shu

A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice

Yuyu Wang ^{a, 1} ,
Houna Duan ^{a, 1} ,
Hongyuan Shi ^{b, *,} ,
Shiwei Zhang ^a ,
Yufang Xu ^a ,
Weiping Zhu ^{a, *,} ,
Xuhong Qian ^a

a.
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
b.
Department of Radiology, Jiangsu Province Hospital, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

shy1188@126.com

wpzhu@ecust.edu.cn

Received Date: 18 April 2020
Revised Date: 18 May 2020
Accepted Date: 19 May 2020
Available Online: 25 May 2020

Figures(4)

A highly sensitive fluorescent sensor ZnDN was designed, synthesized and used for tracking intracellular zinc ions in various living cells and direct imaging of prostatic tissue in mice. ZnDN was prepared from the heterocyclic-fused naphthalimide fluorophore, and the zinc receptor, N, N-bis(2-pyridylmethyl)ethylenediamine (BPEN). Upon addition of Zn²⁺ to the solutions of ZnDN, a remarkable fluorescence enhancement was observed, which could be attributed to the photo-induced electron transfer (PET) mechanism. Since ZnDN exhibited high sensitivity toward Zn²⁺ in phosphate buffer solution, with a limit of detection of 4.0×10^-9 mol/L, it was further applied for the imaging of exogenous and endogenous Zn²⁺ in different living cells. Living cells imaging experiments suggested that ZnDN could image the changes of intracellular free zinc ions, and could be used for two-photon imaging. Moreover, flow cytometry suggested that ZnDN could distinguish cancerous prostate cells from normal cells. Animal experiments indicated that ZnDN had the potential in imaging prostate tissue in vivo.
- Fluorescent probe,
- Zinc ion,
- Prostate cells,
- Naphthalimide,
- In vivo imaging
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