Citation: Fan Jiaqi, Fan Yu, Wei Zijin, Li Yijing, Li Xiangdan, Wang Lei, Wang Hao. Transformable peptide nanoparticles inhibit the migration of N-cadherin overexpressed cancer cells[J]. Chinese Chemical Letters, ;2020, 31(7): 1787-1791. doi: 10.1016/j.cclet.2020.03.065 shu

Transformable peptide nanoparticles inhibit the migration of N-cadherin overexpressed cancer cells

Fan Jiaqi ^a,b ,
Fan Yu ^b ,
Wei Zijin ^a,b ,
Li Yijing ^b ,
Li Xiangdan ^a,*, ,
Wang Lei ^b,*, ,
Wang Hao ^b,*,

a.
Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science, SouthCentral University for Nationalities, Wuhan 430074, China
b.
CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology(NCNST), Beijing 100190, China

lixiangdan@mail.scuec.edu.cn

wanglei@nanoctr.cn

wanghao@nanoctr.cn

Received Date: 26 February 2020
Revised Date: 24 March 2020
Accepted Date: 24 March 2020
Available Online: 30 March 2020

Figures(4)

About 90% cancer-related mortality results from the cancer metastasis, which generally undergoes after epithelial-mesenchymal transition (EMT) process. N-Cadherin, overexpressed on cancer cell surface during EMT, can enhance the migration of cancer cells. Herein, we design and synthesize a transformable peptide BP-KLVFF-SWTLYTPSGQSK (BFS) that can block N-cadherin for inhibiting cancer migration and metastasis. The peptide BFS consists of three modules including (1) the hydrophobic bis-pyrene (BP) unit for forming and locating nanoparticles, (2) the KLVFF peptide sequence for forming and stabilizing fibrous structures and (3) the targeting peptide sequence SWTLYTPSGQSK that can specifically bind to Ncadherin. The peptide BFS can form nanoparticles in PBS, which can transform to nanofibers when targeting and binding to N-cadherin. The nanofibers inhibit the migration of N-cadherin overexpressed MDA-MB-436 cancer cells. The peptide BFS shows 83.6% inhibiting rate in cells wound healing assay. In addition, the inhibition rate is 67.9% when the BFS applied in transwell migration assay. These results indicate that the BFS has excellent ability to inhibit migration of cancer cells. This self-assembly strategy could be potentially utilized to regulate the key protein during EMT for inhibiting the tumor metastasis.
- Self-assembly,
- Peptide,
- N-Cadherin,
- Cancer,
- Ligand-receptor interaction
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