Citation: Zhijie Fang, Zhe Su, Wenjing Qin, Hao Li, Bing Fang, Wei Du, Qiong Wu, Bo Peng, Peng Li, Haidong Yu, Lin Li, Wei Huang. Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H₂S/viscosity in the brain of drosophila Parkinson's disease model[J]. Chinese Chemical Letters, ;2020, 31(11): 2903-2908. doi: 10.1016/j.cclet.2020.03.063 shu

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H₂S/viscosity in the brain of drosophila Parkinson's disease model

Zhijie Fang ^{a, 1} ,
Zhe Su ^{a, 1} ,
Wenjing Qin ^a ,
Hao Li ^a ,
Bing Fang ^b ,
Wei Du ^b ,
Qiong Wu ^{a, *,} ,
Bo Peng ^{b, *,} ,
Peng Li ^b ,
Haidong Yu ^b ,
Lin Li ^{a, *,} ,
Wei Huang ^{a, b}

a.
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing 211816, China
b.
Shaanxi Institute of Flexible Electronics (SIFE) & Institute of Biomedical Materials & Engineering (IBME), Northwestern Polytechnical University (NPU), Xi'an 710072, China

iamqwu@njtech.edu.cn

iambpeng@nwpu.edu.cn

iamlli@njtech.edu.cn

Received Date: 28 February 2020
Revised Date: 21 March 2020
Accepted Date: 23 March 2020
Available Online: 28 March 2020

Figures(7)

H₂S is an essential gas signal molecule in cells, and viscosity is a key internal environmental parameter. Recent studies have shown that H₂S acts as a cytoarchitecture agent and gas transmitter in many tissues, e.g., as a regulator of neuroendocrine in the brain for mediating vascular tone in blood vessels. Mitochondrial viscosity is an important parameter for judging whether mitochondrial function is normal. It has been reported that oxidative stress and mitochondrial dysfunction are connected with Parkinson's disease (PD), and the protective role of H₂S in PD models has been extensively demonstrated. Herein, Mito-HS, a new two-photon fluorescent probe was demonstrated to detect cross-talk between the two channels of mitochondrial viscosity and H₂S content. Moreover, this probe could detect the relative amount of and changes in mitochondrial H₂S in situ due to the reduced mitochondrial targeting ability after reaction with H₂S. The results show that H₂S in mitochondria is inversely related to viscosity. The PD model has a lower H₂S in mitochondria and a higher mitochondrial viscosity than did the normal. This result is important for our deep understanding of PD and its causes.
- Two-photon,
- H₂S,
- Viscosity,
- Fluorogenic probe,
- Parkinson's disease
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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H2S/viscosity in the brain of drosophila Parkinson's disease model

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通讯作者: 陈斌, bchen63@163.com

Two-photon dual-channel fluorogenic probe for in situ imaging the mitochondrial H₂S/viscosity in the brain of drosophila Parkinson's disease model