Citation: Shuangxi Wang, Bicong Zhou, Na Wang, Changmin Yu, Naidi Yang, Jian Chen, Qiong Wu, Chengwu Zhang, Lin Li, Wei Huang. Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic[J]. Chinese Chemical Letters, ;2020, 31(11): 2897-2902. doi: 10.1016/j.cclet.2020.03.037 shu

Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic

Shuangxi Wang ^a ,
Bicong Zhou ^a ,
Na Wang ^a ,
Changmin Yu ^{a, *,} ,
Naidi Yang ^a ,
Jian Chen ^c ,
Qiong Wu ^a ,
Chengwu Zhang ^a ,
Lin Li ^{a, *,} ,
Wei Huang ^{a, b}

a.
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing 211800, China
b.
Shaanxi Institute of Flexible Electronics (SIFE), Northwestern Polytechnical University (NPU), Xi'an 710072, China
c.
Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan University of Science and Technology, Xiangtan 411201, China

iamcmyu@njtech.edu.cn

iamlli@njtech.edu.cn

Received Date: 14 January 2020
Revised Date: 11 March 2020
Accepted Date: 13 March 2020
Available Online: 16 March 2020

Figures(6)

Directly monitoring mitophagy-specific viscosity dynamic in living cells is of great significance but remains challenging. Herein, this study reported a novel mitochondria-targeted fluorescent probe DPAC-DY based on vibration-induced emission (VIE) for monitoring viscosity changes during mitochondrial autophagy. This probe contained N, N'-diphenyl-dihydrodibenzo[a, c]phenazine (DPAC) as the VIE core and two positively charged pyridinium moieties for mitochondria anchoring. As the ambient viscosity increased, the vibration of DPAC-DY could be hindered, and subsequently resulting in the enhancement of fluorescence emission. In vitro and intracellular experiments indicated that the probe DPAC-DY showed highly sensitive response to viscosity due to VIE mechanism. Importantly, by virtue of this probe, in situ and real-time visualization of the specific viscosity dynamics during the mitochondrial autophagy process was achieved. Thus, this work provides a novel strategy for VIE-based viscosity response sensors applied to specific organelles and offers a platform for in-depth study of mitochondrial viscosity-related diseases.
- Vibration-induced emission,
- Autophagy,
- Viscosity,
- Fluorescence detection
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