Citation: Xiao Huaming, Liu Ping, Zheng Shujian, Wang Xian, Ding Jun, Feng Yuqi. Screening of amino acids in dried blood spots by stable isotope derivatization-liquid chromatography-electrospray ionization mass spectrometry[J]. Chinese Chemical Letters, ;2020, 31(9): 2423-2427. doi: 10.1016/j.cclet.2020.03.003 shu

Screening of amino acids in dried blood spots by stable isotope derivatization-liquid chromatography-electrospray ionization mass spectrometry

    * Corresponding author.
    E-mail address: yqfeng@whu.edu.cn (Y. Feng).
  • Received Date: 2 January 2020
    Revised Date: 28 February 2020
    Accepted Date: 28 February 2020
    Available Online: 15 September 2020

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  • Direct infusion mass spectrometry (DIMS) is a powerful technique in clinical diagnosis for screening neonatal amino acid metabolic disorders from dried blood spots (DBS). However, DIMS sometimes generated false-positive results for analysis of amino acids. In this work, we utilized a stable isotope derivatization method, combining with liquid chromatography tandem mass spectrometry (SID-LC-MS), to improve the specificity for screening amino acids in DBS specimens. A pair of isotope reagents, p-(dimethylamino)phenyl isothiocyanate (DMAP-NCS) and 4-isothiocyanato-N, N-bis(methyl-[2H2])aniline ([2H4]DMAP-NCS), was synthesized and used to label amino acids in DBS specimens. The [2H4]DMAP-NCS labelled amino acid standards were used as internal standards to compensate the matrix effect. This method was validated by measuring linearity, recovery and accuracy. The results showed that the developed SID-LC-MS method can be used for sensitive and selective determination of 12 diagnostically important amino acids in DBS specimens.
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