Citation: Dong Hongming, Tang Congli, He Ziyu, Liu Hongmei, Xu Yuyue, Huang Hao, Yang Gaojian, Xiao Ziqi, Li Song, Deng Yan, Chen Zhu, Chen Hui, Qin Zuodong, Negrin Yasser Perera, He Nongyue. Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization[J]. Chinese Chemical Letters, ;2020, 31(7): 1812-1816. doi: 10.1016/j.cclet.2020.03.002 shu

Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization

Dong Hongming ^a,1 ,
Tang Congli ^a,1 ,
He Ziyu ^a ,
Liu Hongmei ^a ,
Xu Yuyue ^a ,
Huang Hao ^a ,
Yang Gaojian ^a ,
Xiao Ziqi ^a ,
Li Song ^a,*, ,
Deng Yan ^a,b ,
Chen Zhu ^a ,
Chen Hui ^a ,
Qin Zuodong ^c ,
Negrin Yasser Perera ^d ,
He Nongyue ^a,b,*,

a.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
b.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
c.
Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, Hunan University of Science and Engineering, Yongzhou 425199, China
d.
China-Cuba Biotechnology Joint Innovation Center(CCBJIC), Yongzhou Zhong Gu Biotechnology Co., Yongzhou 425000, China

sosong1980@gmail.com

nyhe1958@163.com

Received Date: 22 December 2019
Revised Date: 29 February 2020
Accepted Date: 1 March 2020
Available Online: 2 March 2020

Figures(4)

Diarrhea, as a global public health problem, causes a large number of infections and deaths every year. Although Escherichia coli (E. coli) is one of the normal flora microorganisms in the human intestinal tract, it has five pathogenic bacteria types that can cause human diarrhea, known as diarrheagenic E. coli. When people are infected, rapid and accurate diagnosis, along with timely treatment, are especially important. Here, we introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. Results show that the detection sensitivities of enterohemorrhagic E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, enteroinvasive E. coli and enteroaggregative E. coli were 1.3×10³ CFU/mL, 2×10⁴ CFU/mL, 4×10⁴ CFU/mL, 7.2×10⁴ CFU/mL and 1.7 CFU/mL respectively. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. Compared with other methods, BMB array has great application potential.
- Diarrheagenic E. coli,
- Rapid detection,
- Multiplex PCR,
- Barcoded magnetic bead,
- Detect multiple target
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