Citation: Wang Wen, Liu Xiaolong, Chen Huan, Ling Ting, Xia Tian, Liu Xiumei, Liu Jing, Otkur Wuxiyar, Shi Xianzhe, Qi Huan, Chen Di, Piao Hai-Long. Cholesterol as a functional metabolite cooperates with metadherin in cancer cells[J]. Chinese Chemical Letters, ;2020, 31(7): 1831-1834. doi: 10.1016/j.cclet.2019.12.027 shu

Cholesterol as a functional metabolite cooperates with metadherin in cancer cells

Wang Wen ^a,b ,
Liu Xiaolong ^a ,
Chen Huan ^a,b ,
Ling Ting ^a,b ,
Xia Tian ^a ,
Liu Xiumei ^a ,
Liu Jing ^a ,
Otkur Wuxiyar ^a ,
Shi Xianzhe ^a,b ,
Qi Huan ^a ,
Chen Di ^a ,
Piao Hai-Long ^a,b,*,

a.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

hpiao@dicp.ac.cn

Received Date: 24 October 2019
Revised Date: 16 December 2019
Accepted Date: 17 December 2019
Available Online: 19 December 2019

Figures(3)

Protein-metabolite interactions (PMIs) play important roles in various biological processes, especially in disease progression. However, due to the complexity of living cells, it is very difficult to identify specific PMIs. Herein, we chose one oncogenic factor, metadherin (MTDH), as a bait to identify its in vivo interacting metabolites in cancer cells. Cholesterol is an important metabolite and essential structural component of cell membranes. It could also drive several diseases including cancer. Interestingly, we found that cholesterol robustly interacted with MTDH and downregulated the expression of MTDH in cancer cells. Furthermore, MTDH disturbed metabolite alterations under cholesterol treatment in MTDH transduced cancer cells. Collectively, our results uncover an undescribed PMI where MTDH, as an oncogenic factor, might positively regulate cancer progression by interacting with cholesterol. This study interprets the theoretical basis of PMI-oriented cancer progression and targeting therapies in clinic.
- Metabolite-protein interaction,
- Cholesterol,
- Metadherin,
- Mass spectrometry
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