Citation: Khan Haroon, Khan Adeel, Liu Yufeng, Wang Su, Bibi Sumaira, Xu Hongpan, Liu Yuan, Durrani Samran, Jin Lian, He Nongyue, Xiong Tao. CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2[J]. Chinese Chemical Letters, ;2019, 30(12): 2201-2204. doi: 10.1016/j.cclet.2019.10.032 shu

CRISPR-Cas13a mediated nanosystem for attomolar detection of canine parvovirus type 2

Khan Haroon ^a,1 ,
Khan Adeel ^a,1 ,
Liu Yufeng ^b,c ,
Wang Su ^b,c ,
Bibi Sumaira ^d ,
Xu Hongpan ^b ,
Liu Yuan ^a ,
Durrani Samran ^e ,
Jin Lian ^f ,
He Nongyue ^a,f,g,*, ,
Xiong Tao ^c,**,

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Department of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
c.
College of Life Science, Yangtze University, Jingzhou 434025, China
d.
Bio Resources Conservation Institute, National Agriculture Research Centre, Islamabad 350000, Pakistan
e.
Laboratory of Biointerface & Biomaterials, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
f.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
g.
National Center for International Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Theranostics, Guangxi Medical University, Nanning 530021, China

^**

nyhe1958@163.com

xiongtao@hotmail.com

Received Date: 3 September 2019
Revised Date: 24 October 2019
Accepted Date: 25 October 2019
Available Online: 31 December 2019

Figures(3)

Canine parvovirus type 2 (CPV-2) infection is the most lethal disease of dogs with higher mortality in puppies worldwide. In today's world, dogs are an integral part of our communities as well as dogs breeding and rearing has become a lucrative business. Therefore, a fast, accurate, portable, and costeffective CPV-2 detection method with the ability for on-site detection is highly desired. In this study, we for the first time proposed a nanosystem for CPV-2 DNA detection with RNA-guided RNA endonuclease Cas13a, which upon activation results in collateral RNA degradation. We expressed LwCas13a in prokaryotic expression system and purified it through nickel column. Activity of Cas13a was verified by RNA-bound fluorescent group while using a quenched fluorescent probe as signals. Further Cas13a was combined with Recombinase polymerase amplification (RPA) and T7 transcription to establish molecular detection system termed specific high-sensitivity enzymatic reporter un-locking (SHERLOCK) for sensitive detection of CPV-2 DNA. This nanosystem can detect 100 amol/L CPV-2 DNA within 30 min. The proposed nanosystem exhibited high specificity when tested for CPV-2 and other dog viruses. This CRISPR-Cas13a mediated sensitive detection approach can be of formidable advantage during CPV-2 outbreaks because it is time-efficient, less laborious and does not involve the use of sophisticated instruments.
- CPV,
- CRISPR-Cas13a,
- RPA,
- Attomolar detection,
- SHERLOCK
1. [1]
  H. Cun, P. Miguel, R. Armenio, et al., Hum. Relat. 72 (2019) 778-800. doi: 10.1177/0018726718780210
2. [2]
  A. Treves, C. Bonacic, Trends Ecol. Evolut. 31 (2016) 489-491. doi: 10.1016/j.tree.2016.04.006
3. [3]
  Q.Y. Zhuang, Y. Qiu, Z.H. Pan, et al., Transbound. Emerg. Dis. 66 (2019) 897-907. doi: 10.1111/tbed.13100
4. [4]
  J.D. Figueire, C. Miranda, R. Souto, et al., Arch. Microbiol. 199 (2017) 543-549. doi: 10.1007/s00203-016-1320-7
5. [5]
  H. Wu, X. Li, L. Wang, et al., Virus. Dis. 29 (2018) 113-117. doi: 10.1007/s13337-018-0427-7
6. [6]
  C. Li, J. Tang, Z. Chen, et al., Infect. Genet. Evol. 73 (2019) 242-247. doi: 10.1016/j.meegid.2019.05.004
7. [7]
  D. de la Torre, P.B. Mafla, L. Erazo, et al., Vet. World 11 (2018) 480-487. doi: 10.14202/vetworld.2018.480-487
8. [8]
  C. Janic, M. Enbo, H. Lucas, et al., Science 360 (2018) 436-439. doi: 10.1126/science.aar6245
9. [9]
  L. Cong, F.A. Ran, D. Cox, et al., Science 339 (2013) 819-823. doi: 10.1126/science.1231143
10. [10]
  P. Qin, M. Park, K.J. Alfson, et al., ACS Sens. 4 (2019) 1048-1054. doi: 10.1021/acssensors.9b00239
11. [11]
  R. Jansen, J.D.A. Embden, W. Gaastra, et al., Mol. Microbiol. 43 (2002) 1565-1575. doi: 10.1046/j.1365-2958.2002.02839.x
12. [12]
  L. Liu, X. Li, J. Ma, et al., Cell 170 (2017) 714-726. doi: 10.1016/j.cell.2017.06.050
13. [13]
  O.O. Abudayyeh, J.S. Gootenberg, S. Konermann, et al., Science 353 (2016) aaf5573. doi: 10.1126/science.aaf5573
14. [14]
  A.E. Seletsky, A. O'Connell, M.R. Knight, et al., Nature 538 (2016) 270-273. doi: 10.1038/nature19802
15. [15]
  J.S. Gootenberg, O.O. Abudayyeh, J.W. Lee, et al., Science 356 (2017) 438-442. doi: 10.1126/science.aam9321
16. [16]
  A.A. Hatoum, Clin. Chem. 64 (2018) 1681-1683. doi: 10.1373/clinchem.2018.295485
17. [17]
  K. Uwatoko, M. Sunairi, M. Nakajima, et al., Vet. Microbiol. 43 (1995) 315-323. doi: 10.1016/0378-1135(94)00102-3
18. [18]
  T.X.Castro, C.M.A.Uchoa, M.C.Albuquerque, Int.J.Appl.Res.Vet.Med.5 (2007)72-76.
19. [19]
  M. Mochizuki, M.V.S. Gabriel, H. Nakatani, et al., Res. Vet. Sci. 55 (1993) 60-63. doi: 10.1016/0034-5288(93)90035-E
20. [20]
  Y. Suna, Y. Chenga, P. Lina, et al., Mol. Cell. Probes 38 (2018) 7-12. doi: 10.1016/j.mcp.2018.02.004
21. [21]
  C. Desario, N. Decaro, M. Campolo, et al., J. Virol. Methods 126 (2005) 179-185. doi: 10.1016/j.jviromet.2005.02.006
22. [22]
  Y. Geng, J. Wang, L. Liu, et al., BMC Vet. Res. 13 (2017) 311. doi: 10.1186/s12917-017-1232-z
23. [23]
  S.R. Kersting, V. Rausch, F.F. Bier, M.N. Rosenegk, Microchim. Acta 181 (2014) 1715-1723. doi: 10.1007/s00604-014-1198-5
24. [24]
  J.S. Gootenberg, O.O. Abudayyeh, M.J. Kellner, et al., Science 360 (2018) 439-444. doi: 10.1126/science.aaq0179
25. [25]
  C. Desario, N. Decaro, M. Campolo, et al., J. Virol. Methods 126 (2005) 179-185. doi: 10.1016/j.jviromet.2005.02.006
26. [26]
  J. Wang, C. Liu, R. Li, Arch. Virol. 161 (2016) 1015-1018. doi: 10.1007/s00705-015-2738-y
27. [27]
  H.K. Mukhopadhyay, S. Amsaveni, S.L. Matta, et al., Lett. Appl. Microbiol. 55 (2012) 202-209. doi: 10.1111/j.1472-765X.2012.03284.x
28. [28]
  V. Gupta, S. Chakravarti, V. Chander, et al., Arch. Virol. 162 (2017) 1995-2001. doi: 10.1007/s00705-017-3321-5
29. [29]
  R.K. Daher, G. Stewart, M. Boissinot, M.G. Bergeron, Clin. Chem. 62 (2016) 947. doi: 10.1373/clinchem.2015.245829
30. [30]
  J. Wang, J. Wang, R. Li, et al., BMC Vet. Res. 13 (2017) 241. doi: 10.1186/s12917-017-1180-7
31. [31]
  S.S. Jevdenic, D. Trailovic, B. Vidic, M. Jovanovic, Acta Veterinaria-Beograd 56 (2006) 515-527. doi: 10.2298/AVB0606515S
32. [32]
  A.F. Ortega, J.S. Martinez-Castaneda, L.G. Bautista-Gomez, et al., Braz. J. Microbiol. 48 (2017) 769-773. doi: 10.1016/j.bjm.2017.03.008
33. [33]
  L.B. Liu, J.C. Wang, Y.Y. Geng, et al., Mol. Cell. Probes 39 (2018) 41-46. doi: 10.1016/j.mcp.2018.04.004
34. [34]
  J. Thomas, M. Singh, T.K. Goswami, et al., Biologicals 49 (2017) 51-56. doi: 10.1016/j.biologicals.2017.06.009
35. [35]
  M. Mochizuki, S. Gabriel, M.C. Nakatani, et al., Res. Vet. Sci. 55 (1993) 60-63. doi: 10.1016/0034-5288(93)90035-E
36. [36]
  Q. Fan, Q. Xie, Z.X. Xie, et al., J. Virol. Methods 186 (2012) 43-48. doi: 10.1016/j.jviromet.2012.08.007
1. [1]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
2. [2]
  Rui Wang , Yang Liang , Julius Rebek Jr. , Yang Yu . Stabilization and detection of labile reaction intermediates in supramolecular containers. Chinese Chemical Letters, 2024, 35(6): 109228-. doi: 10.1016/j.cclet.2023.109228
3. [3]
  Zihong Li , Jie Cheng , Ping Huang , Guoliang Wu , Weiying Lin . Activatable photoacoustic bioprobe for visual detection of aging in vivo. Chinese Chemical Letters, 2024, 35(4): 109153-. doi: 10.1016/j.cclet.2023.109153
4. [4]
  Tiankai Sun , Hui Min , Zongsu Han , Liang Wang , Peng Cheng , Wei Shi . Rapid detection of nanoplastic particles by a luminescent Tb-based coordination polymer. Chinese Chemical Letters, 2024, 35(5): 108718-. doi: 10.1016/j.cclet.2023.108718
5. [5]
  Yuxin Xiao , Xiaowei Wang , Yutong Yin , Fangchao Yin , Jinchao Li , Zhiyuan Hou , Mashooq Khan , Rusong Zhao , Wenli Wu , Qiongzheng Hu . Distance-based lateral flow biosensor for the quantitative detection of bacterial endotoxin. Chinese Chemical Letters, 2024, 35(12): 109718-. doi: 10.1016/j.cclet.2024.109718
6. [6]
  Haibo Wan , Zhengzhong Lv , Jicai Jiang , Xuefeng Cheng , Qingfeng Xu , Haibin Shi , Jianmei Lu . Multidimensional detection of roxarsone via AIE-based sulfates. Chinese Chemical Letters, 2025, 36(3): 110023-. doi: 10.1016/j.cclet.2024.110023
7. [7]
  Yue Li , Minghao Fan , Conghui Wang , Yanxun Li , Xiang Yu , Jun Ding , Lei Yan , Lele Qiu , Yongcai Zhang , Longlu Wang . 3D layer-by-layer amorphous MoS_x assembled from [Mo₃S₁₃]^2- clusters for efficient removal of tetracycline: Synergy of adsorption and photo-assisted PMS activation. Chinese Chemical Letters, 2024, 35(9): 109764-. doi: 10.1016/j.cclet.2024.109764
8. [8]
  Yingying Yan , Wanhe Jia , Rui Cai , Chun Liu . An AIPE-active fluorinated cationic Pt(Ⅱ) complex for efficient detection of picric acid in aqueous media. Chinese Chemical Letters, 2024, 35(5): 108819-. doi: 10.1016/j.cclet.2023.108819
9. [9]
  Xinqiong Li , Guocheng Rao , Xi Peng , Chan Yang , Yanjing Zhang , Yan Tian , Xianghui Fu , Jia Geng . Direct detection of C9orf72 hexanucleotide repeat expansions by nanopore biosensor. Chinese Chemical Letters, 2024, 35(5): 109419-. doi: 10.1016/j.cclet.2023.109419
10. [10]
  Guorong Li , Yijing Wu , Chao Zhong , Yixin Yang , Zian Lin . Predesigned covalent organic framework with sulfur coordination: Anchoring Au nanoparticles for sensitive colorimetric detection of Hg(Ⅱ). Chinese Chemical Letters, 2024, 35(5): 108904-. doi: 10.1016/j.cclet.2023.108904
11. [11]
  Jun Xiong , Ke-Ke Chen , Neng-Bin Xie , Wei Chen , Wen-Xuan Shao , Tong-Tong Ji , Si-Yu Yu , Yu-Qi Feng , Bi-Feng Yuan . Demethylase-assisted site-specific detection of N¹-methyladenosine in RNA. Chinese Chemical Letters, 2024, 35(5): 108953-. doi: 10.1016/j.cclet.2023.108953
12. [12]
  Dan Ouyang , Huan Huang , Yanting He , Jiajing Chen , Jiali Lin , Zhuling Chen , Zongwei Cai , Zian Lin . Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides. Chinese Chemical Letters, 2024, 35(5): 108885-. doi: 10.1016/j.cclet.2023.108885
13. [13]
  Zhiqiang Liu , Qiang Gao , Wei Shen , Meifeng Xu , Yunxin Li , Weilin Hou , Hai-Wei Shi , Yaozuo Yuan , Erwin Adams , Hian Kee Lee , Sheng Tang . Removal and fluorescence detection of antibiotics from wastewater by layered double oxides/metal-organic frameworks with different topological configurations. Chinese Chemical Letters, 2024, 35(8): 109338-. doi: 10.1016/j.cclet.2023.109338
14. [14]
  Shuang Li , Jiayu Sun , Guocheng Liu , Shuo Zhang , Zhong Zhang , Xiuli Wang . A new Keggin-type polyoxometallate-based bifunctional catalyst for trace detection and pH-universal photodegradation of phenol. Chinese Chemical Letters, 2024, 35(8): 109148-. doi: 10.1016/j.cclet.2023.109148
15. [15]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
16. [16]
  Xin Dong , Jing Liang , Zhijin Xu , Huajie Wu , Lei Wang , Shihai You , Junhua Luo , Lina Li . Exploring centimeter-sized crystals of bismuth-iodide perovskite toward highly sensitive X-ray detection. Chinese Chemical Letters, 2024, 35(6): 108708-. doi: 10.1016/j.cclet.2023.108708
17. [17]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
18. [18]
  Jia-Mei Qin , Xue Li , Wei Lang , Fu-Hao Zhang , Qian-Yong Cao . An AIEgen nano-assembly for simultaneous detection of ATP and H₂S. Chinese Chemical Letters, 2024, 35(6): 108925-. doi: 10.1016/j.cclet.2023.108925
19. [19]
  Yunxin Li , Jinghui Zhang , Jisen Chen , Feng Zhu , Zhiqiang Liu , Peng Bao , Wei Shen , Sheng Tang . Detection of SARS-CoV-2 based on artificial intelligence-assisted smartphone: A review. Chinese Chemical Letters, 2024, 35(7): 109220-. doi: 10.1016/j.cclet.2023.109220
20. [20]
  Ting-Ting Huang , Jin-Fa Chen , Juan Liu , Tai-Bao Wei , Hong Yao , Bingbing Shi , Qi Lin . A novel fused bi-macrocyclic host for sensitive detection of Cr₂O₇²⁻ based on enrichment effect. Chinese Chemical Letters, 2024, 35(7): 109281-. doi: 10.1016/j.cclet.2023.109281

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(624)
HTML views(14)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142