Citation: Yan Yu, Zhang Xiaodong, Zhang Xinfu, Li Ning, Man Huizi, Chen Lingcheng, Xiao Yi. Ratiometric sensing lysosomal pH in inflammatory macrophages by a BODIPY-rhodamine dyad with restrained FRET[J]. Chinese Chemical Letters, ;2020, 31(5): 1091-1094. doi: 10.1016/j.cclet.2019.10.025 shu

Ratiometric sensing lysosomal pH in inflammatory macrophages by a BODIPY-rhodamine dyad with restrained FRET

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

zhangxinfu@dlut.edu.cn

xiaoyi@dlut.edu.cn

Received Date: 26 July 2019
Revised Date: 18 October 2019
Accepted Date: 22 October 2019
Available Online: 24 October 2019

Figures(4)

Inflammation, as the pathophysiological response of body to harmful stimuli, leads to changes in cellular microenvironment. To research pH changes in lysosomes of macrophages during inflammation, we designed a FRET (Förster resonance energy transfer) based probe, BDP-RhB. The probe showed good lysosome targeting ability, wide response range of pH from 8.0 to 4.0 with significant ratio (I₅₈₂/I₅₁₈) change from 0.6 to 3.4, and good reversibility and sustainability. By applying BDP-RhB, we found a decrease of lysosomal pH of macrophages during inflammation.
- pH probe,
- Inflammation,
- Lysosome,
- FRET,
- Macrophages
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