Citation: Wang Hong, Wei Jing, Zhang Chonghua, Zhang Yuan, Zhang Yubin, Li Lin, Yu Changmin, Zhang Peisheng, Chen Jian. Red carbon dots as label-free two-photon fluorescent nanoprobes for imaging of formaldehyde in living cells and zebrafishes[J]. Chinese Chemical Letters, ;2020, 31(3): 759-763. doi: 10.1016/j.cclet.2019.09.021 shu

Red carbon dots as label-free two-photon fluorescent nanoprobes for imaging of formaldehyde in living cells and zebrafishes

Wang Hong ^a,1 ,
Wei Jing ^b,1 ,
Zhang Chonghua ^a,**, ,
Zhang Yuan ^a ,
Zhang Yubin ^a ,
Li Lin ^b ,
Yu Changmin ^b,*, ,
Zhang Peisheng ^a ,
Chen Jian ^a,c,**,

a.
Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Lab of Advanced Materials for New Energy Storage and Conversion, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China
b.
Key Laboratory of Flexible Electronics(KLOFE) & Institute of Advanced Materials(IAM), Nanjing Tech University, Nanjing 211800, China
c.
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China

^**

chonghua1226@163.com

iamcmyu@njtech.edu.cn

cj0066@gmail.com

Received Date: 23 July 2019
Revised Date: 9 September 2019
Accepted Date: 10 September 2019
Available Online: 11 September 2019

Figures(5)

Direct, in situ selective detection of intracellular formaldehyde (FA) is of great significance for understanding its function in FA-related diseases. Herein, red carbon dots (RCD) are reported as label-free two-photon fluorescent nanoprobes for detecting and imaging of FA. Upon addition of FA, the -NH₂ groups of RCD could quickly and specially react with aldehydes to form Schiff base and then the strong fluorescence of RCD with blue-shift emission is recovery due to the destruction of the hydrogen bond interaction between RCD and water. In addition, the nanoprobes exhibit outstanding photostability, rapid response (< 1 min), high sensitivity (~9.9 μmol/L) and excellent selectivity toward FA over other aldehyde group compounds. Notably, owing to the good cell-membrane permeability and biocompatibility, as well as the large two-photon absorption cross-section, the as-prepared RCD can be used as label-free nanoprobes for selectively detecting and imaging FA in living cells and zebrafishes through one-photon and two-photon excitation. Moreover, RCD could stain the tissue of zebrafishes at depths interval of up to 240 mm under two-photon excitation. This research implied that RCD are promising tools for directly and in situ imaging FA in vivo, thus providing critical insights into FA-related pathophysiological processes.
- Carbon dots (CDs),
- Two-photon excitation,
- Formaldehyde (FA),
- Intracellular imaging,
- Zebrafishes
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