Citation: Wu Yuanheng, Chen Qiang, Liu Shuang, Xiao Hua, Zhang Menglin, Zhang Xinfeng. Surface molecular imprinting on g-C₃N₄ photooxidative nanozyme for improved colorimetric biosensing[J]. Chinese Chemical Letters, ;2019, 30(12): 2186-2190. doi: 10.1016/j.cclet.2019.08.014 shu

Surface molecular imprinting on g-C₃N₄ photooxidative nanozyme for improved colorimetric biosensing

a.
College of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China
b.
College of Environment and Ecology, Chengdu University of Technology, Chengdu 610059, China

zhangxinfeng09@cdut.cn

Received Date: 17 June 2019
Revised Date: 11 August 2019
Accepted Date: 12 August 2019
Available Online: 13 December 2019

Figures(5)

Graphitic carbon nitride (g-C₃N₄), as a visible-light-active organic semiconductor, has attracted growing attentions in photocatalysis and photoluminescence-based biosensing. Here, we demonstrated the intrinsic photooxidase activity of g-C₃N₄ and then surface molecular imprinting on g-C₃N₄ nanozymes was achieved for improved biosensing. Upon blue LED irradiation, the g-C₃N₄ exhibited superior enzymatic activity for oxidation of chromogenic substrate like 3, 3', 5, 5'-tetramethylbenzidine (TMB) without destructive H₂O₂. The oxidation was mainly ascribed to ^·O₂^- that was generated during light irradiation. The surface molecular imprinting on g-C₃N₄ can lead to an over 1000-fold alleviation in matrix-interference from serum samples, 4-fold improved enzymatic activity as well as enhanced substrate specificity comparing with bare g-C₃N₄ during colorimetric sensing. Also, the MIP-g-C₃N₄ possesses a high affinity to TMB with a K_m value of only 22 μmol/L, much lower than other comment nanozymes like AuNPs, Fe₃O₄ NPs, etc. It was successfully applied for detection of cysteine in serum sample with satisfactory recoveries.
- Molecularly imprinted polymer,
- Graphitic carbon nitride,
- Nanoenzyme,
- Colorimetry,
- Biosensing
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Surface molecular imprinting on g-C3N4 photooxidative nanozyme for improved colorimetric biosensing

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通讯作者: 陈斌, bchen63@163.com

Surface molecular imprinting on g-C₃N₄ photooxidative nanozyme for improved colorimetric biosensing