Citation: Shi Huan, Jin Tian, Zhang Jiewen, Huang Xiaoting, Tan Chunyan, Jiang Yuyang, Tan Ying. A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease Ⅲ-aided recycling amplification[J]. Chinese Chemical Letters, ;2020, 31(1): 155-158. doi: 10.1016/j.cclet.2019.06.020 shu

A novel aptasensor strategy for protein detection based on G-quadruplex and exonuclease Ⅲ-aided recycling amplification

Shi Huan ^a,b,1 ,
Jin Tian ^a,b,1 ,
Zhang Jiewen ^a ,
Huang Xiaoting ^a,b ,
Tan Chunyan ^a,b ,
Jiang Yuyang ^a,c,*, ,
Tan Ying ^a,b,*,

a.
State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Biology, the Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
b.
Department of Chemistry, Tsinghua University, Beijing 100084, China
c.
Department of Pharmacology and Pharmaceutical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China

jiangyy@sz.tsinghua.edu.cn

tan.ying@sz.tsinghua.edu.cn

Received Date: 17 April 2019
Revised Date: 6 June 2019
Accepted Date: 10 June 2019
Available Online: 11 January 2019

Figures(4)

The detection of biomarkers is of great significance in the diagnosis of numerous diseases, especially cancer. Herein, we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads, DNA G-quadruplex, and exonuclease Ⅲ (Exo Ⅲ). In the presence of a target protein, a label-free single strand DNA (ssDNA) hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target. Subsequently, ssDNA initiates the Exo Ⅲ-aided recycling to amplify the fluorescence signal, which was caused by N-methylmesoporphyrin Ⅸ (NMM) insertion into the G-quadruplex structure. This proposed strategy combines the excellent specificity between the aptamer and target, high sensitivity of the fluorescence signal by G-quadruplex and Exo Ⅲaided recycling amplification. We selected (50-1200 nmol/L) MUC1, a common tumor biomarker, as the proof-of-concept target to test the specificity of our aptasensor. Results reveal that the sensor sensitively and selectively detected the target protein with limits of detection (LODs) of 3.68 and 12.83 nmol/L in buffer solution and 10% serum system, respectively. The strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.
- Aptasensor,
- Exonuclease Ⅲ-aided recycling,
- amplification,
- G-quadruplex,
- Magnetic beads,
- MUC1
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