Citation: Luo Jing, Li Ting, Yang Minghui. Detection protein biomarker with gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles as electrochemical probes[J]. Chinese Chemical Letters, ;2020, 31(1): 202-204. doi: 10.1016/j.cclet.2019.05.051 shu

Detection protein biomarker with gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles as electrochemical probes

Luo Jing ^a ,
Li Ting ^a,* ,
Yang Minghui ^b,*

a.
Department of General Surgery, The Second Xiang-ya Hospital, Central South University, Changsha 410011, China
b.
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

csu.edu.cn

yangminghui@csu.edu.cn

Received Date: 19 April 2019
Revised Date: 23 May 2019
Accepted Date: 24 May 2019
Available Online: 25 January 2019

Figures(4)

Gold nanoparticles functionalized hollow mesoporous Prussian blue nanoparticles (Au@HMPB NPs) were synthesized and its peroxidase-like activity was explored for electrochemical probe. The Au@HMPB NPs can reduce H₂O₂ at low detection potential of -0.1 V with high sensitivity. After physically adsorption of antibodies onto the gold nanoparticle surface, the functionalized nanoparticles were turned into immuno-probe. The soluble α-chain of interleukin-2 (IL-2) receptor (sCD25) was chosen as a model protein biomarker to test the performance of the probe. sCD25 in the samples were captured and enriched by capture anti-CD25 antibody functionalized magnetic nanospheres. Detection antibody functionalized Au@HMPB can then be linked onto the nanospheres and generate electrochemical current towards H₂O₂ reduction. The electrochemical responses to 1 mmol/L H₂O₂ was increased with the increasing concentration of CD25.
- God nanoparticles,
- Prussian blue,
- Electrochemical,
- Peroxidase,
- CD25
1. [1]
  K.A. Smith, Annu. Rev. Cell Biol. 5(1989) 397-425. doi: 10.1146/annurev.cb.05.110189.002145
2. [2]
  Z. Huang, J. Fang, J. Gu, Y. Yan, J. Zhou, Veterinary Immunol. Immunopathol.140(2011) 102-109. doi: 10.1016/j.vetimm.2010.11.021
3. [3]
  L.A. Rubin, F. Galli, W.C. Greene, D.L. Nelson, G. Jay, Cytokine 2(1990) 330-336. doi: 10.1016/1043-4666(90)90062-X
4. [4]
  S.H. Hsiao, M.S. Lee, H.Y. Lin, et al., Acta Otolaryngol. 129(2009) 1519-1523. doi: 10.3109/00016480902849427
5. [5]
  M. Kami, T. Matsumura, Y. Tanaka, et al., Leuk. Lymphoma 38(2000) 533-540. doi: 10.3109/10428190009059272
6. [6]
  E. Boleslawski, S. BenOthman, S. Grabar, et al., Clin. Transplant. 22(2008) 494-501. doi: 10.1111/j.1399-0012.2008.00815.x
7. [7]
  C. Shen, S. Liu, X. Li, D. Zhao, M. Yang, Microchim. Acta 185(2018) 547. doi: 10.1007/s00604-018-3086-x
8. [8]
  G. Wang, H. Wang, S. Cao, et al., Microchim. Acta 186(2019) 96. doi: 10.1007/s00604-018-3223-6
9. [9]
  X. Pei, B. Zhang, J. Tang, et al., Anal. Chim. Acta 758(2013) 1-18. doi: 10.1016/j.aca.2012.10.060
10. [10]
  K. Zhang, K. Zeng, C. Shen, S. Tian, M. Yang, Microchim. Acta 185(2018) 225. doi: 10.1007/s00604-018-2754-1
11. [11]
  L. Zhao, L. Teng, J. Zhang, H. Li, Chin. Chem. Lett. 30(2019) 1035-1037.
12. [12]
  Y. Chai, X. Li, M. Yang, Microchim. Acta 186(2019) 316. doi: 10.1007/s00604-019-3412-y
13. [13]
  Y. Si, Z. Sun, N. Zhang, et al., Anal. Chem. 86(2014) 10406-10414. doi: 10.1021/ac5028885
14. [14]
  Y. Si, N. Zhang, Z. Sun, et al., Analyst 139(2014) 5466-5471. doi: 10.1039/C4AN01074D
15. [15]
  W. Xiang, G. Wang, S. Cao, et al., Microchim. Acta 185(2018) 335. doi: 10.1007/s00604-018-2867-6
16. [16]
  X. Li, C. Shen, M. Yang, A. Rasooly, Anal. Chem. 90(2018) 4764-4769. doi: 10.1021/acs.analchem.8b00023
17. [17]
  D. Tang, B. Su, J. Tang, J. Ren, G. Chen, Anal. Chem. 82(2010) 1527-1534. doi: 10.1021/ac902768f
18. [18]
  Y. Zhang, B. Huang, F. Yu, et al., Microchim. Acta 185(2018) 86. doi: 10.1007/s00604-017-2631-3
19. [19]
  Y. Lin, L. Hu, L. Yin, L. Guo, Sensor. Actuat. B-Chem. 210(2015) 513-518. doi: 10.1016/j.snb.2014.12.107
20. [20]
  R. Li, D. Guo, J. Ye, M. Zhang, Analyst 140(2015) 3746-3752. doi: 10.1039/C4AN02352H
21. [21]
  W. Zhang, D. Ma, J. Du, Talanta 120(2014) 362-367. doi: 10.1016/j.talanta.2013.12.028
22. [22]
  R. Ojani, P. Hamidi, J.B. Raoof, Chin. Chem. Lett. 27(2016) 481-486. doi: 10.1016/j.cclet.2015.12.030
23. [23]
  K. Zeng, M. Yang, Y.N. Liu, A. Rasooly, Anal. Methods 10(2018) 3951-3957. doi: 10.1039/C8AY01456F
1. [1]
  Peng Jin , Sili Lin , Dongmei Wang , Jinsong Fan , Qingyun Liu , Kun Li . Valence-band hybridization endows the reaction specificity of AgPd nanozyme for exclusive peroxidase mimicking and improved sensing performance. Chinese Chemical Letters, 2025, 36(11): 110916-. doi: 10.1016/j.cclet.2025.110916
2. [2]
  Xinwei Hu , Yong Zeng , Jiongdong Ma , Han Diao , Fei-Xiao Chen , Mu Chen , Shou-Kun Zhang , Chengzhi Jin , Shao-Fei Ni , Zhixiong Ruan . Electrochemical selenylation of tyrosine oligopeptides to benzo[b]furan-functionalized peptides. Chinese Chemical Letters, 2026, 37(3): 111928-. doi: 10.1016/j.cclet.2025.111928
3. [3]
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4. [4]
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5. [5]
  Qingyun Yang , Yue Ma , Quanyi Ye , Yiqing Liu , Yuhong Luo , Yongbo Wu , Zhiguang Xu , Xiaoming Lin . Prussian blue analogues derived MO/MFe2O4 (M = Ni, Cu, Zn) nanoparticles as a high-performance anode material for enhanced lithium storage. Chinese Journal of Structural Chemistry, 2025, 44(8): 100631-100631. doi: 10.1016/j.cjsc.2025.100631
6. [6]
  Siwei Lv , Tantian Tan , Xinyue Li , Siyan Zhang , Mingyuan Zhang , Minghao Li , Hangshuo Guo , Zhaorong Li , Liangjie Dong , Fengshuo Zhang , Junlong Zhao . Competition of the “King of Transboundary Medicine”. University Chemistry, 2024, 39(9): 102-108. doi: 10.12461/PKU.DXHX202403034
7. [7]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
8. [8]
  Zeqiu Chen , Limiao Cai , Jie Guan , Zhanyang Li , Hao Wang , Yaoguang Guo , Xingtao Xu , Likun Pan . Advanced electrode materials in capacitive deionization for efficient lithium extraction. Acta Physico-Chimica Sinica, 2025, 41(8): 100089-0. doi: 10.1016/j.actphy.2025.100089
9. [9]
  Zhaoyu Wen , Na Han , Yanguang Li . Recent Progress towards the Production of H₂O₂ by Electrochemical Two-Electron Oxygen Reduction Reaction. Acta Physico-Chimica Sinica, 2024, 40(2): 2304001-0. doi: 10.3866/PKU.WHXB202304001
10. [10]
  Jie WEI , Qing ZHOU , Dandan DING , Xiang JING , Fei LI . Photothermal toxicity of Prussian blue nanoparticles to cervical cancer cells. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2345-2357. doi: 10.11862/CJIC.20240435
11. [11]
  Jiayu Xu , Meng Li , Baoxia Dong , Ligang Feng . Fully fluorinated hybrid zeolite imidazole/Prussian blue analogs with combined advantages for efficient oxygen evolution reaction. Chinese Chemical Letters, 2024, 35(6): 108798-. doi: 10.1016/j.cclet.2023.108798
12. [12]
  Mingyue Luo , Kehui Zhang , Honghong Rao , Jianying Li , Xin Xue , Panpan Sun , Xiaoquan Lu , Zhonghua Xue . A simplified ratiometric fluorescent sensing strategy for enhanced detection of alkaline phosphatase employing Prussian blue nanozymes and commercially available chromogen. Chinese Chemical Letters, 2025, 36(9): 110703-. doi: 10.1016/j.cclet.2024.110703
13. [13]
  Xiaonan LI , Hui HAN , Yihan ZHANG , Jing XIONG , Tingting GUO , Juanzhi YAN . A viologen‐based Cd(Ⅱ) coordination polymer: Self‐assembly, thermochromism, and electrochemical property. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1439-1444. doi: 10.11862/CJIC.20240376
14. [14]
  Qiangqiang Zhou , Lili Sun , Yu-Jie Guo , Bo Zhou , Chunfang Zhang , Sen Xin , Le Yu , Gaohong Zhai . First-principles study on the electrochemical properties of Na-ion-intercalatable heterostructures formed by transitional metal dichalcogenides and blue phosphorus. Chinese Chemical Letters, 2025, 36(7): 110187-. doi: 10.1016/j.cclet.2024.110187
15. [15]
  Zhijuan Niu , Peizhe Sun , Kwangnak Koh , Changping Li . Ultrasensitive electrochemical sensor based on para-sulfonatocalix[4]arene functionalized gold nanoparticles for sulfamethazine detection. Chinese Chemical Letters, 2025, 36(11): 110844-. doi: 10.1016/j.cclet.2025.110844
16. [16]
  Wei GUO , Zhuoyi GUO , Xiaoxin LI , Wei ZHANG , Juanzhi YAN , Tingting GUO . Electrochemical sensor based on a Co(Ⅱ)-based metal-organic framework for the detection of Cd²⁺ and Pb²⁺. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1889-1902. doi: 10.11862/CJIC.20250097
17. [17]
  Mingyue Luo , Xin Xue , Kehui Zhang , Honghong Rao , Baodui Wang , Zhentong Zhu , Jinhai Fan , Zhonghua Xue . Hollow mesoporous Prussian blue nanozymes with intrinsic self-chromogenicity and photothermal amplification as a pH-adaptive dual-modal biosensor for one-step glucose monitoring. Chinese Chemical Letters, 2026, 37(3): 111496-. doi: 10.1016/j.cclet.2025.111496
18. [18]
  Yuwan Lu , Xiaodan Zhang , Yuming Huang . Dual-site Se/NC specific peroxidase-like nanozyme for highly sensitive methimazole detection. Chinese Chemical Letters, 2025, 36(4): 110129-. doi: 10.1016/j.cclet.2024.110129
19. [19]
  Shaobin He , Xiaoyun Guo , Qionghua Zheng , Huanran Shen , Yuan Xu , Fenglin Lin , Jincheng Chen , Haohua Deng , Yiming Zeng , Wei Chen . Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay. Chinese Chemical Letters, 2025, 36(4): 110096-. doi: 10.1016/j.cclet.2024.110096
20. [20]
  Jin-Ao Li , Nana Pan , Li Lu , Yifan Wei , Wenchang Hu , Zhiyang Zhang , Houda Amrani , Fang Huang , Xiaojuan Wang . Ligand-bridged nanoassemblies boost the peroxidase-mimicking activity of gold nanoclusters. Chinese Chemical Letters, 2026, 37(1): 111806-. doi: 10.1016/j.cclet.2025.111806

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