Citation: Ding Cong, Chen Yage, Li Haozheng, Wang Bingyao, Wei Qi, Tang Huajun, Jia Shaokang, He Zhiyong, Wang Ping, Zhou Xiang. Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne[J]. Chinese Chemical Letters, ;2019, 30(7): 1393-1396. doi: 10.1016/j.cclet.2019.03.046 shu

Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne

Ding Cong ^a,1 ,
Chen Yage ^b,c,1 ,
Li Haozheng ^b,c,1 ,
Wang Bingyao ^a ,
Wei Qi ^a ,
Tang Huajun ^b,c ,
Jia Shaokang ^a ,
He Zhiyong ^a,*, ,
Wang Ping ^b,c ,
Zhou Xiang ^a,*,

a.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of the Ministry of Education, WuhanUniversity, Wuhan 430072, China
b.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
c.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China

hezy@whu.edu.cn

xzhou@whu.edu.cn

Received Date: 26 February 2019
Revised Date: 25 March 2019
Accepted Date: 26 March 2019
Available Online: 27 July 2019

Figures(4)

We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future
- Raman probe,
- SRS,
- Cell imaging,
- Photo-stability feature
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