Citation: He Sitian, He Leiliang, Liu Beibei, Yu Songcheng, Liu Li'e, Tian Yongmei, Wang Jia, Ding Lihua, Wang Yilin, Qu Lingbo, Yu Fei, Wu Yongjun. Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum[J]. Chinese Chemical Letters, ;2019, 30(5): 1031-1034. doi: 10.1016/j.cclet.2019.03.013 shu

Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum

He Sitian ^a ,
He Leiliang ^a ,
Liu Beibei ^b ,
Yu Songcheng ^a ,
Liu Li'e ^a ,
Tian Yongmei ^a ,
Wang Jia ^a ,
Ding Lihua ^a ,
Wang Yilin ^a ,
Qu Lingbo ^c ,
Yu Fei ^a,*, ,
Wu Yongjun ^a,*,

a.
College of Public Health, Zhengzhou University, Zhengzhou 450001, China
b.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China
c.
Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou 450001, China

yufei@zzu.edu.cn

wuyongjun135@126.com

Received Date: 29 December 2018
Revised Date: 5 March 2019
Accepted Date: 6 March 2019
Available Online: 8 May 2019

Figures(5)

DNA methyltransferase 1 (DNMT1) is a useful biomarker for lung cancer in early clinical diagnosis. A rapid magnetic chemiluminescence immunoassay (MCLIA) for DNMT1 in human serum has been developed. Horseradish peroxidase(HRP)-second-Abwasused to labeled polyclonal antibodies of anti-DNMT1. DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction. In magnetic field, nonspecific materials can be separated. After luminescent substrate luminol-H₂O₂-BIP was added, the relative light unit (RLU) of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample. The correlative variables involved in the MCLIA valuewere optimized and the methodological evaluationwas carriedout. Afteroptimization, in the range of 0.5-128 ng/mL, the linear regression equation was y=0.5014x + 1.769 (x was logC_DNMT1, y was relative luminescence units (RLU)/RLU₀), and the limit of detection was 0.01 ng/mL. The RSD of intra- and interassays were 15.8%-16.9% and 14.3%-18.1%, respectively. The recovery was from 70.0% to 106.2%. Furthermore, paralleled with purchasable enzyme-linked immunosorbent assay (ELISA) kits, MCLEIA had lower detection limit, wider linear range and shorter detection time. Therefore, the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample.
- DNA methyltransferase 1,
- Biomarker,
- Chemiluminescence immunoassay,
- Magnetic particles,
- Human serum
1. [1]
  P. Prasad, S.A. Mittal, J. Chongtham, S. Mohanty, T. Srivastava, Stem Cells 35(2017) 1468-1478. doi: 10.1002/stem.2621
2. [2]
  S.K. Botla, S. Savant, P. Jandaghi, et al., Cancer Res. 76(2016) 4149-4159. doi: 10.1158/0008-5472.CAN-15-0390
3. [3]
  J. Wu, Z. Shuang, J. Zhao, et al., Biomed. Pharmacother. 97(2018) 1275-1281. doi: 10.1016/j.biopha.2017.11.055
4. [4]
  M.Y. Sun, X.X. Yang, W.W. Xu, et al., Genet. Mol. Res. 11(2012) 4330-4341. doi: 10.4238/2012.September.26.1
5. [5]
  W. Zhang, Z. Chang, K.E. Shi, et al., Oncol. Lett. 11(2016) 1995-2000. doi: 10.3892/ol.2016.4193
6. [6]
  C. Zhang, Y. Peng, F. Yang, et al., J. Cancer 7(2016) 446-452. doi: 10.7150/jca.13065
7. [7]
  R.E. Husni, A. Shiba-Ishii, S. Iiyama, et al., Lung Cancer 97(2016) 59-65. doi: 10.1016/j.lungcan.2016.04.018
8. [8]
  H. Li, W. Li, S. Liu, et al., EBioMedicine 13(2016) 125-131. doi: 10.1016/j.ebiom.2016.10.028
9. [9]
  A. Meissner, T.S. Mikkelsen, H. Gu, et al., Nature 454(2008) 766-770. doi: 10.1038/nature07107
10. [10]
  B.Y. Kim, O.S. Kwon, S.A. Joo, et al., Anal. Biochem. 326(2004) 21-24. doi: 10.1016/j.ab.2003.11.009
11. [11]
  K. Chen, J. Liu, S. Liu, et al., Cell 170(2017) 492-506. doi: 10.1016/j.cell.2017.06.042
12. [12]
  C.D. Valdez, J.N. Davis, H.M. Odeh, et al., PLoS One 6(2011) 251-256.
13. [13]
  H. Zhao, L. Wang, W. Li, et al., ACS Appl. Mater. Interfaces 9(2017) 29554-29561. doi: 10.1021/acsami.7b09631
14. [14]
  B. Zhu, J. Ge, S.Q. Yao, Bioorg. Med. Chem. 23(2015) 2917-2927. doi: 10.1016/j.bmc.2015.03.006
15. [15]
  J. Li, G. He, C. Mu, K. Wang, Y. Xiang, Anal. Chim. Acta 986(2017) 131-137. doi: 10.1016/j.aca.2017.07.021
16. [16]
  C. Hu, I. Zeimpekis, K. Sun, et al., Anal. Chem. 88(2016) 4872-4878. doi: 10.1021/acs.analchem.6b00702
17. [17]
  A.G. Pose, E.R. Rodriguez, M.J. Pineiro, et al., J. Immunol. Methods 459(2018) 70-75. doi: 10.1016/j.jim.2018.05.008
18. [18]
  H. Zhang, S. Qi, J. Rao, et al., Luminescence 28(2013) 927-932. doi: 10.1002/bio.v28.6
19. [19]
  Y. Li, Y.Z. Zheng, D.K. Zhang, et al., Chin. Chem. Lett. 28(2017) 184-188. doi: 10.1016/j.cclet.2016.07.020
20. [20]
  M.L. Yuan, T.Y. Jiang, L.P. Du, M.Y. Li, Chin. Chem. Lett. 27(2016) 550-554. doi: 10.1016/j.cclet.2016.02.011
21. [21]
  W.Y. Sun, W.Y. Liu, L.B. Qu, Chin. Chem. Lett. 18(2007) 1107-1110. doi: 10.1016/j.cclet.2007.07.008
22. [22]
  A.R. Rodríguez-Orozco, H. Ruiz-Reyes, N. Medina-Serriteno, Mini Rev. Med. Chem. 10(2010) 113-116.
23. [23]
  H. Zhang, S. Qi, Clin. Chim. Acta 412(2011) 1572-1577. doi: 10.1016/j.cca.2011.05.005
1. [1]
  Yao-Hua Gu , Yu Chen , Qing Li , Neng-Bin Xie , Xue Xing , Jun Xiong , Min Hu , Tian-Zhou Li , Ke-Yu Yuan , Yu Liu , Tang Tang , Fan He , Bi-Feng Yuan . Metabolome profiling by widely-targeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of chronic kidney disease. Chinese Chemical Letters, 2024, 35(11): 109627-. doi: 10.1016/j.cclet.2024.109627
2. [2]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
3. [3]
  Xiaoxiao Wang , Bolun Wang , Fenfen Ji , Jie Yan , Jiacheng Fang , Doudou Zhang , Ji Xu , Jing Ji , Xinran Hao , Hemi Luan , Yanjun Hong , Shulan Qiu , Min Li , Zhu Yang , Wenlan Liu , Xiaodong Cai , Zongwei Cai . Discovery of plasma biomarkers for Parkinson’s disease diagnoses based on metabolomics and lipidomics. Chinese Chemical Letters, 2024, 35(11): 109653-. doi: 10.1016/j.cclet.2024.109653
4. [4]
  Keqiang Shi , Xiujuan Hong , Dongyan Xu , Tao Pan , Huiwen Wang , Hongru Feng , Cheng Guo , Yuanjiang Pan . Analysis of RNA modifications in peripheral white blood cells from breast cancer patients by mass spectrometry. Chinese Chemical Letters, 2025, 36(3): 110079-. doi: 10.1016/j.cclet.2024.110079
5. [5]
  Min Huang , Ru Cheng , Shuai Wen , Liangtong Li , Jie Gao , Xiaohui Zhao , Chunmei Li , Hongyan Zou , Jian Wang . Ultrasensitive detection of microRNA-21 in human serum based on the confinement effect enhanced chemical etching of gold nanorods. Chinese Chemical Letters, 2024, 35(9): 109379-. doi: 10.1016/j.cclet.2023.109379
6. [6]
  Zhaodong WANG . In situ synthesis, crystal structure, and magnetic characterization of a trinuclear copper complex based on a multi-substituted imidazo[1,5-a]pyrazine scaffold. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 597-604. doi: 10.11862/CJIC.20240268
7. [7]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
8. [8]
  Gaowa Xing , Yuting Shang , Xiaorui Wang , Zengnan Wu , Qiang Zhang , Jiebing Ai , Qiaosheng Pu , Ling Lin . A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals. Chinese Chemical Letters, 2024, 35(10): 109491-. doi: 10.1016/j.cclet.2024.109491
9. [9]
  Tiankai Sun , Hui Min , Zongsu Han , Liang Wang , Peng Cheng , Wei Shi . Rapid detection of nanoplastic particles by a luminescent Tb-based coordination polymer. Chinese Chemical Letters, 2024, 35(5): 108718-. doi: 10.1016/j.cclet.2023.108718
10. [10]
  Ruixin Liu , Feng Shi , Yanping Xia , Haibing Zhu , Jiawen Cao , Kai Peng , Chuanli Ren , Juan Li , Zhanjun Yang . Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay. Chinese Chemical Letters, 2024, 35(11): 109664-. doi: 10.1016/j.cclet.2024.109664
11. [11]
  Shaobin He , Xiaoyun Guo , Qionghua Zheng , Huanran Shen , Yuan Xu , Fenglin Lin , Jincheng Chen , Haohua Deng , Yiming Zeng , Wei Chen . Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay. Chinese Chemical Letters, 2025, 36(4): 110096-. doi: 10.1016/j.cclet.2024.110096
12. [12]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
13. [13]
  Huihui LIU , Baichuan ZHAO , Chuanhui WANG , Zhi WANG , Congyun ZHANG . Green synthesis of MIL-101/Au composite particles and their sensitivity to Raman detection of thiram. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 2021-2030. doi: 10.11862/CJIC.20240059
14. [14]
  Xianping Du , Ying Huang , Chen Chen , Zhenhe Feng , Meng Zong . Encapsulating Si particles in multiple carbon shells with pore-rich for constructing free-standing anodes of lithium storage. Chinese Chemical Letters, 2024, 35(12): 109990-. doi: 10.1016/j.cclet.2024.109990
15. [15]
  Di ZHANG , Tianxiang XIE , Xu HE , Wanyu WEI , Qi FAN , Jie QIAO , Gang JIN , Ningbo LI . Construction and antitumor activity of pH/GSH dual-responsive magnetic nanodrug. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 786-796. doi: 10.11862/CJIC.20240329
16. [16]
  Jie Wu , Xiaoqing Yu , Guoxing Li , Su Chen . Engineering particles towards 3D supraballs-based passive cooling via grafting CDs onto colloidal photonic crystals. Chinese Chemical Letters, 2024, 35(4): 109234-. doi: 10.1016/j.cclet.2023.109234
17. [17]
  Peng Zhang , Yitao Yang , Tian Qin , Xueqiu Wu , Yuechang Wei , Jing Xiong , Xi Liu , Yu Wang , Zhen Zhao , Jinqing Jiao , Liwei Chen . Interface engineering of Pt/CeO₂-{100} catalysts for enhancing catalytic activity in auto-exhaust carbon particles oxidation. Chinese Chemical Letters, 2025, 36(2): 110396-. doi: 10.1016/j.cclet.2024.110396
18. [18]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
19. [19]
  Donghui Wu , Qilin Zhao , Jian Sun , Xiurong Yang . Corrigendum to 'Fluorescence immunoassay based on alkaline phosphatase-induced in situ generation of fluorescent non-conjugated polymer dots' [Chin. Chem. Lett. 34 (2023) 107672]. Chinese Chemical Letters, 2024, 35(12): 109881-. doi: 10.1016/j.cclet.2024.109881
20. [20]
  Bing Niu , Honggao Huang , Liwei Luo , Li Zhang , Jianbo Tan . Coating colloidal particles with a well-defined polymer layer by surface-initiated photoinduced polymerization-induced self-assembly and the subsequent seeded polymerization. Chinese Chemical Letters, 2025, 36(2): 110431-. doi: 10.1016/j.cclet.2024.110431

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(687)
HTML views(9)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142