Citation: Wang Chao, Sun Linlin, Zhao Qiang. A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1[J]. Chinese Chemical Letters, ;2019, 30(5): 1017-1020. doi: 10.1016/j.cclet.2019.01.029 shu

A simple aptamer molecular beacon assay for rapid detection of aflatoxin B1

Wang Chao ^a,b ,
Sun Linlin ^a,b ,
Zhao Qiang ^a,b,*,

a.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

qiangzhao@rcees.ac.cn

Received Date: 23 December 2018
Revised Date: 23 January 2019
Accepted Date: 24 January 2019
Available Online: 25 May 2019

Figures(4)

Aflatoxin B1 (AFB1) is a highly toxic mycotoxin, and rapid and sensitive detection of AFB1 is in demand for food safety and environmental analysis. Here we described a simple aptamer molecular beacon assay for rapid detection of aflatoxin B1 (AFB1) by using an aptamer with a fluorescein (FAM) label at the 5' end and a fluorescence quencher (black hole quencher 1, BHQ1) at the 30 end. In the presence of AFB1, the aptamer probe bound with AFB1 and induced a hairpin structure, drawing FAM and BHQ1 into close proximity and leading to fluorescence quenching. This assay allowed for a detection limit of 3.9 nmol/L and a dynamic range from 3.9 nmol/L to 4 μmol/L. Specificity test showed other mycotoxins including ochratoxin A, ochratoxin B, fumonisin B1, fumonisin B2, and zearalenone had negligible influence on detection of AFB1. AFB1 spiked in diluted liquor wine, methanol, or corn flour samples was successfully detected by using this aptamer probe, and the assay showed potential for real sample analysis.
- Aptamer,
- Aflatoxin B1,
- Fluorescence,
- Molecular beacons,
- Mycotoxin,
- Sensor
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