Citation: Wang Zhenyu, Wu Chaoxiong, Fan Ting, Han Xianwei, Wang Qiong, Lei Jincan, Yang Jun. Electroformation and collection of giant liposomes on an integrated microchip[J]. Chinese Chemical Letters, ;2019, 30(2): 353-358. doi: 10.1016/j.cclet.2018.12.001 shu

Electroformation and collection of giant liposomes on an integrated microchip

Wang Zhenyu ^a,b,*, ,
Wu Chaoxiong ^a ,
Fan Ting ^b ,
Han Xianwei ^b ,
Wang Qiong ^b,c ,
Lei Jincan ^c ,
Yang Jun ^b,*,

a.
State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing & the Ministry of Science & Technology, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
b.
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China
c.
Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China

gdwzycq@163.com

bioyangjun@cqu.edu.cn

Received Date: 5 September 2018
Revised Date: 29 November 2018
Accepted Date: 3 December 2018
Available Online: 3 February 2018

Figures(5)

Giant liposome is an important lipid structure widely used in biological and medical fields. In its main preparation method, electroformation, many influencing factors must be optimized for good effect. How to collect the desired giant liposomes is another major issue. In this work, a microchip with a reactor chamber array was used to study the influences of multiple parameters, and a suitable condition could be achieved rapidly and efficiently. A tailor-made collection chamber was also integrated on the chip. Based on the multifactor and multilevel orthogonal experiment, optimal conditions of the lipid solution, buffer solution, and electric signal were achieved with high efficiency. More than one thousand giant liposomes could be formed in each microscale reactor chamber, and most of them were unilamellar. The on-chip collection ratio of giant liposome carriers could also approximate to 40%.
- Giant liposome,
- Microchip,
- ITO,
- Electroformation,
- Collection
1. [1]
  A. Jesorka, O. Orwar, Annu. Rev. Anal. Chem. 1(2008) 801-832. doi: 10.1146/annurev.anchem.1.031207.112747
2. [2]
  K. Kuribayashi, G. Tresset, P. Coquet, H. Fujita, S. Takeuchi, Meas. Sci. Technol.17(2006) 3121-3126. doi: 10.1088/0957-0233/17/12/S01
3. [3]
  P. Walde, K. Cosentino, H. Engel, P. Stano, ChemBioChem 11(2010) 848-865. doi: 10.1002/cbic.201000010
4. [4]
  M.I. Angelova, D.S. Dimitrov, Faraday Discuss. Chem. Soc. 81(1986) 303. doi: 10.1039/dc9868100303
5. [5]
  M.I. Angelova, D.S. Dimitrov, A mechanism of liposome electroformation, in: V. Degiorgio (Ed.), Trends in Colloid and Interface Science Ⅱ, Springer-Verlag, New York, 1988, pp. 59-67.
6. [6]
  H. Bi, B. Yang, L. Wang, W. Cao, X. Han, J. Mater. Chem. A 1(2013) 7125-7130. doi: 10.1039/c3ta10323d
7. [7]
  Q. Wang, X. Zhang, T. Fan, et al., Micromachines 8(2017) 24. doi: 10.3390/mi8010024
8. [8]
  D.S. Dimitrov, M.I. Angelova, Prog. Colloid Polym. Sci. 73(1987) 48-56. doi: 10.1007/3-798-50724-4
9. [9]
  R. Wick, M.I. Angelova, P. Walde, P.L. Luisi, Chem. Biol. 3(1996) 105-111. doi: 10.1016/S1074-5521(96)90286-0
10. [10]
  P. Bucher, A. Fischer, P.L. Luisi, T. Oberholzer, P. Walde, Langmuir 14(1998) 2712-2721. doi: 10.1021/la971318g
11. [11]
  L. RuthMontes, A. Alonso, F.M. Goñi, L.A. Bagatolli, Biophys. J. 93(2007) 3548-3554. doi: 10.1529/biophysj.107.116228
12. [12]
  T. Pott, H. Bouvrais, P. Méléard, Chem. Phys. Lipids 154(2008) 115-119. doi: 10.1016/j.chemphyslip.2008.03.008
13. [13]
  V. Pereno, D. Carugo, L. Bau, et al., ACS Omega 2(2017) 994-1002. doi: 10.1021/acsomega.6b00395
14. [14]
  M.I. Angelova, S. Soléau, P. Méléard, F. Faucon, P. Bothorel, Preparation of giant vesicles by external AC electric fields. Kinetics and applications, in: C. Helm, M. Lösche, H. Möhwald (Eds.), Trends in Colloid and Interface Science VI, Springer-Verlag, New York, 1992, pp. 127-131.
15. [15]
  L. Mathivet, S. Cribier, P.F. Devaux, Biophys. J. 70(1996) 1112-1121. doi: 10.1016/S0006-3495(96)79693-5
16. [16]
  Y.M.S. Micheletto, C.M. Marques, Nd.Pd. Silveira, A.P. Schroder, Langmuir 32(2016) 8123-8130. doi: 10.1021/acs.langmuir.6b01679
17. [17]
  M.D. Collins, S.E. Gordon, J. Vis. Exp. (2013) 50227.
18. [18]
  P. Taylor, C. Xu, P.D.I. Fletcher, V.N. Paunov, Chem. Commun. (2003) 1732.
19. [19]
  P. Taylor, C. Xu, P.D.I. Fletcher, V.N. Paunov, Phys. Chem. Chem. Phys. 5(2003) 4918-4922. doi: 10.1039/b308082j
20. [20]
  D.J. Estes, M. Mayer, Colloid Surf. B 42(2005) 115-123. doi: 10.1016/j.colsurfb.2005.01.016
21. [21]
  T.J. Politano, V.E. Froude, B. Jing, Y. Zhu, Colloid Surf. B 79(2010) 75-82. doi: 10.1016/j.colsurfb.2010.03.032
22. [22]
  Q. Li, X. Wang, S. Ma, Y. Zhang, X. Han, Colloid Surf. B 147(2016) 368-375. doi: 10.1016/j.colsurfb.2016.08.018
23. [23]
  D.J. Estes, M. Mayer, BBA-Biomembranes 1712(2005) 152-160. doi: 10.1016/j.bbamem.2005.03.012
24. [24]
  D.J. Estes, S.R. Lopez, A.O. Fuller, M. Mayer, Biophys. J. 91(2006) 233-243. doi: 10.1529/biophysj.105.076398
25. [25]
  Y. Okumura, H. Zhang, T. Sugiyama, Y. Iwata, J. Am. Chem. Soc. 129(2007) 1490-1491. doi: 10.1021/ja068127x
26. [26]
  Y. Okumura, Y. Iwata, Membranes 1(2011) 109-118. doi: 10.3390/membranes1020109
27. [27]
  M.L. Berre, A. Yamada, L. Reck, Y. Chen, D. Baigl, Langmuir 24(2008) 2643-2649. doi: 10.1021/la703391q
28. [28]
  A. Diguet, M.L. Berre, Y. Chen, D. Baigl, Small. 5(2009) 1661-1666. doi: 10.1002/smll.v5:14
29. [29]
  Y. Okumura, T. Sugiyama, Membranes 1(2011) 184-194. doi: 10.3390/membranes1030184
30. [30]
  T. Shimanouchi, H. Umakoshi, R. Kuboi, Langmuir 25(2009) 4835-4840. doi: 10.1021/la8040488
31. [31]
  T.L. Marin, BostonThe Proceedings of the COMSOL Users Conference2006, The Proceedings of the COMSOL Users Conference (2006) 1-8.
32. [32]
  R.D. Deegan, O. Bakajin, T.F. Dupont, et al., Nature 389(1997) 827-829. doi: 10.1038/39827
33. [33]
  Z. Wang, N. Hu, L.H. Yeh, et al., Colloid Surf. B 110(2013) 81-87. doi: 10.1016/j.colsurfb.2013.04.042
34. [34]
  D. Constantin, C. Ollinger, M. Vogel, T. Salditt, Eur. Phys. J. E 18(2005) 273-278. doi: 10.1140/epje/e2005-00028-7
35. [35]
  J. Leng, S.U. Egelhaaf, M.E. Cates, Europhys. Lett. 59(2002) 311-317. doi: 10.1209/epl/i2002-00243-1
36. [36]
  K.S. Horger, Formation of Physiologically Relevant Liposomes, the University of Michigan, 2013, pp. 20-22.
37. [37]
  F.M. Menger, M.I. Angelova, Acc. Chem. Res. 31(1998) 789-797. doi: 10.1021/ar970103v
38. [38]
  Y. Yamashita, M. Oka, T. Tanaka, M. Yamazaki, Biochim. Biophys. Acta 1561(2002) 129-134. doi: 10.1016/S0005-2736(02)00338-3
39. [39]
  S.M. Nomura, S. Kondoh, W. Asayama, et al., J. Biotechnol. 133(2008) 190-195. doi: 10.1016/j.jbiotec.2007.08.023
40. [40]
  K. Shigyou, K.H. Nagai, T. Hamada, Langmuir 32(2016) 13771-13777. doi: 10.1021/acs.langmuir.6b02448
1. [1]
  Shangda Qu , Yiming Yuan , Xu Ye , Wentao Xu . High sensitivity artificial synapses using printed high-transmittance ITO fibers for neuromorphic computing. Chinese Chemical Letters, 2024, 35(12): 110030-. doi: 10.1016/j.cclet.2024.110030
2. [2]
  Wangyan Hu , Ke Li , Xiangnan Dou , Ning Li , Xiayan Wang . Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography. Chinese Chemical Letters, 2024, 35(4): 108806-. doi: 10.1016/j.cclet.2023.108806
3. [3]
  Gaowa Xing , Yuxuan Li , Hongren Yao , Qiang Zhang , Zengnan Wu , Caihou Lin , Jin-Ming Lin . Tryptophan accumulation and inflammation of glioblastoma cells in a multicomponent microchip for gut-brain-axis simulation. Chinese Chemical Letters, 2025, 36(12): 111035-. doi: 10.1016/j.cclet.2025.111035
4. [4]
  He Zhao , Qiangqiang Dong , Fengxue Liu , Ning Wang , Lijun Wang , Mingzhao Chen , Zhilong Jiang , Die Liu , Jun Wang , Pingshan Wang , Yiming Li . Dovetail joint strategy for constructing giant multi-propeller supramolecular architectures. Chinese Chemical Letters, 2026, 37(2): 112051-. doi: 10.1016/j.cclet.2025.112051
5. [5]
  Chen Lian , Si-Han Zhao , Hai-Lou Li , Xinhua Cao . A giant Ce-containing poly(tungstobismuthate): Synthesis, structure and catalytic performance for the decontamination of a sulfur mustard simulant. Chinese Chemical Letters, 2024, 35(10): 109343-. doi: 10.1016/j.cclet.2023.109343
6. [6]
  Cheng Cheng , Nasir Ali , Ji Liu , Juan Qiao , Ming Wang , Li Qi . Construction of degradable liposome-templated microporous metal-organic frameworks with commodious space for enzymes. Chinese Chemical Letters, 2024, 35(11): 109812-. doi: 10.1016/j.cclet.2024.109812
7. [7]
  Cheng-Zhe Gao , Hao-Ran Jia , Tian-Yu Wang , Xiao-Yu Zhu , Xiaofeng Han , Fu-Gen Wu . A dual drug-loaded tumor vasculature-targeting liposome for tumor vasculature disruption and hypoxia-enhanced chemotherapy. Chinese Chemical Letters, 2025, 36(1): 109840-. doi: 10.1016/j.cclet.2024.109840
8. [8]
  Yulin Chen , Guangchao Wang , Fengjin Zhou , Zhifeng Yin , Fuming Shen , Weizong Weng , Hao Zhang , Yingying Jiang , Xinru Liu , Yonghui Deng , Yuan Chen , Ke Xu , Jiacan Su . Targeting TRPA1 with liposome-encapsulated drugs anchored to microspheres for effective osteoarthritis treatment. Chinese Chemical Letters, 2025, 36(5): 110053-. doi: 10.1016/j.cclet.2024.110053
9. [9]
  Yu Pang , Min Wang , Ning-Hua Yang , Min Xue , Yong Yang . One-pot synthesis of a giant twisted double-layer chiral macrocycle via [4 + 8] imine condensation and its X-ray structure. Chinese Chemical Letters, 2024, 35(10): 109575-. doi: 10.1016/j.cclet.2024.109575

Get Citation

PDF

XML

Metrics

PDF Downloads(6)
Abstract views(1749)
HTML views(42)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142