Citation: Yang Gaojian, Lai Yuxuan, Xiao Ziqi, Tang Congli, Deng Yan. Ultrasensitive electrochemical immunosensor of carcinoembryonic antigen based on gold-label silver-stain signal amplification[J]. Chinese Chemical Letters, ;2018, 29(12): 1857-1860. doi: 10.1016/j.cclet.2018.11.030 shu

Ultrasensitive electrochemical immunosensor of carcinoembryonic antigen based on gold-label silver-stain signal amplification

Yang Gaojian ^a ,
Lai Yuxuan ^a ,
Xiao Ziqi ^a ,
Tang Congli ^a ,
Deng Yan ^a,b,*,

a.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
b.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China

hndengyan@126.com

Received Date: 18 November 2018
Revised Date: 30 November 2018
Accepted Date: 30 November 2018
Available Online: 30 December 2018

Figures(4)

A novel gold-label silver-stain electrochemical immunosensor was developed based on polythioninegold nanoparticles (PTh-Au) modified glassy carbon electrode (GCE) as a platform and secondary antibody labeled Au NPs (Ab₂-Au) as immumoprobe for carcinoembryonic antigen (CEA) detection. The sandwich-type biosensor adopted anodic stripping voltammetry to detect silver stripping signal when the Ab₂-Au of the formed immunocomplexes were stained with silver. The optimized detection conditions were investigated. The effect of different electrochemical responses at various concentrations of CEA was checked by anodic stripping voltammetry. This immunosensor showed a low detection limit of 0.055 ng/mL and a wide linear calibration of 0.1-120 ng/mL (R²=0.99856). Moreover, this immunoassay also existed the advantages of good reproducibility, stability and selectivity. Thus, this immunosensing protocol may provide a potential application for effective clinical detection of CEA.
- Gold-label silver-stain,
- Carcinoembryonic antigen,
- Sandwich-type immunoassay,
- Electrochemical immunosensor,
- Anodic stripping voltammetry
1. [1]
  I.E. Tothill, Semin. Cell Dev. Biol. 20 (2009) 55-62. doi: 10.1016/j.semcdb.2009.01.015
2. [2]
  C. Sun, L. Ma, Q. Qian, et al., Analyst 139 (2014) 4216-4222. doi: 10.1039/C4AN00479E
3. [3]
  Y.S. Gao, J.K. Xu, L.M. Lu, et al., RSC Adv. 5 (2015) 86910-86918. doi: 10.1039/C5RA16618G
4. [4]
  S.Y. Xue, H.Y. Yi, P. Jing, W.J. Xu, RSC Adv. 5 (2015) 77454-77459. doi: 10.1039/C5RA15038H
5. [5]
  M.J. Engelen, H.W. de Bruijn, H. Hollema, et al., Gynecol Oncol. 78 (2000) 16-20. doi: 10.1006/gyno.2000.5811
6. [6]
  Y.X. Lai, Y. Deng, G.J. Yang, et al., J. Biomed. Nanotechnol. 14 (2018) 1688-1694. doi: 10.1166/jbn.2018.2617
7. [7]
  K.J. Huang, D.J. Niu, W.Z. Xie, W. Wang, Anal. Chim. Acta 659 (2010) 102-108. doi: 10.1016/j.aca.2009.11.023
8. [8]
  S. Ge, F. Yu, L. Ge, et al., Analyst 137 (2012) 4727-4733. doi: 10.1039/c2an35967g
9. [9]
  F. Naghibalhossaini, P. Ebadi, Cancer Lett. 234 (2006) 158-167. doi: 10.1016/j.canlet.2005.03.028
10. [10]
  J.D. Wulfkuhle, L.A. Liotta, E.F. Petricoin, Nat. Rev. Cancer 3 (2003) 267-275. doi: 10.1038/nrc1043
11. [11]
  W.J. Zhou, J. Su, Y.Q. Chai, R. Yuan, Y. Xiang, Biosens. Bioelectron. 53 (2014) 494-498. doi: 10.1016/j.bios.2013.10.020
12. [12]
  C. Luo, W. Wen, F. Lin, et al., RSC Adv. 5 (2015) 10994-10999. doi: 10.1039/C4RA14833A
13. [13]
  Q. Yu, X. Wang, Y. Duan, Anal. Chem. 86 (2014) 1518-1524. doi: 10.1021/ac402973n
14. [14]
  X.L. Ren, J.J. Ge, S. Li, et al., J. Biomed. Nanotechnol. 13 (2017) 1425-1434. doi: 10.1166/jbn.2017.2432
15. [15]
  H.W. Yang, M. Liu, H.R. Jiang, et al., J. Biomed. Nanotechnol.13 (2017) 655-664. doi: 10.1166/jbn.2017.2386
16. [16]
  Z.Q. Bai, X.L. Ren, Z. Gong, et al., Chin. Chem. Lett. 28 (2017) 1901-1904. doi: 10.1016/j.cclet.2017.05.005
17. [17]
  T.T. Li, H. Yi, Y. Liu, et al., J. Biomed. Nanotechnol. 14 (2018) 150-160. doi: 10.1166/jbn.2018.2491
18. [18]
  L.L. Chen, Z.J. Zhang, P. Zhang, X.M. Zhang, A.H. Fu, Sensor. Actuat. B -Chem.155 (2011) 557-561. doi: 10.1016/j.snb.2011.01.007
19. [19]
  R. Wang, J.J. Feng, Y.D. Xue, L. Wu, A.J. Wang, et al., Sensor. Actuat. B -Chem. 254 (2018) 1174-1181. doi: 10.1016/j.snb.2017.08.009
20. [20]
  T. Feng, X. Chen, X. Qiao, et al., Anal. Biochem. 494 (2016) 101-107. doi: 10.1016/j.ab.2015.11.004
21. [21]
  W. Lu, C. Qian, L. Bi, et al., Biosens. Bioelectron. 53 (2014) 346-354. doi: 10.1016/j.bios.2013.10.007
22. [22]
  M.Rizwan, S.Elma, S.A.Lim, M.U.Ahmed, Biosens.Bioelectron.107 (2018)211-217. doi: 10.1016/j.bios.2018.02.037
23. [23]
  D. Wu, H. Ma, Y. Zhang, et al., ACS Appl. Mater. Interfaces 7 (2015) 18786-18793. doi: 10.1021/acsami.5b05443
24. [24]
  Y. Wang, Y. Wang, D. Wu, et al., Sensor. Actuat. B -Chem. 255 (2018) 125-132. doi: 10.1016/j.snb.2017.07.129
25. [25]
  Y. Yang, Q. Liu, Y. Liu, et al., Biosens. Bioelectron. 90 (2017) 31-38. doi: 10.1016/j.bios.2016.11.029
26. [26]
  H. Wang, Z. Ma, Microchimica Acta 184 (2017) 1045-1050. doi: 10.1007/s00604-017-2101-y
27. [27]
  Y. Liu, Y.X. Lai, G.J. Yang, et al., J. Biomed. Nanotechnol. 13 (2017) 1253-1259. doi: 10.1166/jbn.2017.2424
28. [28]
  Y. Huang, J. Tan, L.J. Cui, et al., Biosens. Bioelectron. 102 (2018) 560-567. doi: 10.1016/j.bios.2017.11.037
29. [29]
  Y.X. Lai, C.X. Zhang, Y. Deng, et al., Chin. Chem. Lett. 29 (2018), doi:http://dx.doi.org/10.1016/j.cclet.2018.07.011. doi: 10.1016/j.cclet.2018.07.011
30. [30]
  Y. Xing, X.Z. Feng, L.P. Zhang, et al., Int. J. Nanomed. 12 (2017) 3171-3179. doi: 10.2147/IJN
31. [31]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Sensor. Actuat. B -Chem. 252 (2017) 44-54. doi: 10.1016/j.snb.2017.05.148
32. [32]
  Y. Liu, K.K. Liu, H.M. Dong, et al., Nanosci. Nanotechnol. Lett. 8 (2016) 785-790. doi: 10.1166/nnl.2016.2264
33. [33]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Biosens. Bioelectron. 92 (2017) 87-94. doi: 10.1016/j.bios.2017.02.002
34. [34]
  Z. Liu, W. Li, G.C. Han, et al., J. Electrochem. Soc. 161 (2014) B75-B80. doi: 10.1149/2.022405jes
35. [35]
  T. Springer, X.C. Song, M.L. Ermini, J. Lamacová, J. Homola, Anal. Bioanal. Chem. 9409 (2017) 4087-4097.
36. [36]
  X.H. Wang, H.S. Peng, W. Yang, Z.D. Ren, Y.A. Liu, Nanosci. Nanotechnol. Lett. 9 (2017) 227-232. doi: 10.1166/nnl.2017.2317
37. [37]
  S.Y. Pang, N. Sheng, J.P. Wang, et al., J. Biomed. Nanotechnol. 13 (2017) 1178-1209. doi: 10.1166/jbn.2017.2423
38. [38]
  H.D. Cui, D.H. Hu, J.N. Zhang, et al., Chin. Chem. Lett. 28 (2017) 1391-1398. doi: 10.1016/j.cclet.2016.12.038
39. [39]
  J. Yang, F.J. Nian, Z.R. Guo, T.T. Bai, G. Peng, Nanosci. Nanotechnol. Lett. 9 (2017) 1491-1496. doi: 10.1166/nnl.2017.2503
40. [40]
  Y.P. Jia, B.Y. Ma, X.W. Wei, Z.Y. Qian, Chin. Chem. Lett. 28 (2017) 691-702. doi: 10.1016/j.cclet.2017.01.021
41. [41]
  Y. Li, J. Sun, Q.P. Chen, L. Zhu, Nanosci. Nanotechnol. Lett. 9 (2017) 982-987. doi: 10.1166/nnl.2017.2430
42. [42]
  J. Zhou, L. Du, L. Zou, et al., Sensor. Actuat. B -Chem. 197 (2014) 220-227. doi: 10.1016/j.snb.2014.02.009
43. [43]
  J. Li, H. Xie, Y. Liu, et al., Talanta 144 (2015) 404-410. doi: 10.1016/j.talanta.2015.06.065
44. [44]
  P. Duangkaew, S. Tapaneeyakorn, C. Apiwat, et al., Biosens. Bioelectron. 74 (2015) 673-679. doi: 10.1016/j.bios.2015.07.004
45. [45]
  K.N. Zhu, Z.Y. Zhu, H.O. Zhou, J.Y. Zhang, S.Y. Liu, Chin. Chem. Lett. 28 (2017) 1276-1284. doi: 10.1016/j.cclet.2017.03.020
46. [46]
  T. Bai, P. Lu, K. Zhang, et al., J. Biomed. Nanotechnol. 13 (2017) 1178-1209. doi: 10.1166/jbn.2017.2423
47. [47]
  X. Miao, S. Zou, H. Zhang, L.S. Ling, Sensor. Actuat. B -Chem. 191 (2014) 396-400. doi: 10.1016/j.snb.2013.10.016
48. [48]
  Y. Sun, Y. Bai, W. Yang, C.Q. Sun, Electrochimica Acta 52 (2007) 7352-7361. doi: 10.1016/j.electacta.2007.06.007
49. [49]
  C. Yang, Q. Wang, Y. Xiang, R. Yuan, Y.Q. Chai, Sensor. Actuat. B -Chem. 197 (2014) 149-154. doi: 10.1016/j.snb.2014.02.036
50. [50]
  F.Y. Kong, M.T. Xu, J.J. Xu, H.Y. Chen, Talanta 85 (2011) 2620-2625. doi: 10.1016/j.talanta.2011.08.028
51. [51]
  X. Cai, S. Weng, R. Guo, et al., Biosens. Bioelectron. 81 (2016) 173-180. doi: 10.1016/j.bios.2016.02.066
52. [52]
  H. Wang, Z. Ma, Microchimica Acta 184 (2017) 3247-3253. doi: 10.1007/s00604-017-2287-z
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