Citation: Huang Li, Zhang Di, Jiao Lirong, Su Enben, He Nongyue. A new quality control method for lateral flow assay[J]. Chinese Chemical Letters, ;2018, 29(12): 1853-1856. doi: 10.1016/j.cclet.2018.11.028 shu

A new quality control method for lateral flow assay

Huang Li ^a,b,c ,
Zhang Di ^a,b ,
Jiao Lirong ^a,b,c ,
Su Enben ^c ,
He Nongyue ^a,b,*,

a.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing 210096, China
c.
Getein Biotechnology Co., Ltd., Nanjing 210000, China

nyhe@seu.edu.cn

Received Date: 20 November 2018
Revised Date: 27 November 2018
Accepted Date: 28 November 2018
Available Online: 28 December 2018

Figures(4)

Precision and repeatability are challenging issues in point of care testing (POCT) analysis. Herein, we proposed a lateral flow assay (LFA) based on internal quality control microspheres to realize the accurate diagnosis of HbA1c in human body. Fluorescein cy5 decorated microspheres are used as labels for HbA1c detection, and BSA-fluorescein isothiocyanate (FITC) decorated microspheres are used as internal quality control labels. One test line was employed in the strip for the detection of glycosylated hemoglobin (HbA1c). This method can eliminate the interference of environmental factors (temperature, humidity, etc.) to LFA in the process of chromatography, and improve the precision and accuracy of HbA1c detection. The CV for detection of low concentration HbA1c was 1.05%, and the CV for detection of high concentration HbA1c was 0.69%. We envision the method to have great prospect in in vitro diagnosis (IVD).
- Point of care testing,
- Lateral flow assay,
- HbA1c,
- Fluorescein isothiocyanate,
- Precision
1. [1]
  R.H. Tang, H. Yang, J.R. Choi, et al., Crit. Rev. Biotechnol. 37 (2016) 411-428.
2. [2]
  C. Parolo, A. Merkoci, Chem. Soc. Rev. 42 (2013) 450-457. doi: 10.1039/C2CS35255A
3. [3]
  W.C. Huang, K.H. Wu, H.C. Hung, et al., J. Nanosci. Nanotechnol. 18 (2018) 7190. doi: 10.1166/jnn.2018.16020
4. [4]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Sensor. Actuat. B -Chem. 252 (2017) 44-54. doi: 10.1016/j.snb.2017.05.148
5. [5]
  A. Murali, N. Haridharan, P.S. Babu, S. Jayaveni, S.N. Jaisankar, J. Nanosci. Nanotechnol. 18 (2018) 959-966. doi: 10.1166/jnn.2018.13959
6. [6]
  L.L. Cao, C. Fang, R.S. Zeng, et al., Biosens. Bioelectron. 92 (2017) 87-94. doi: 10.1016/j.bios.2017.02.002
7. [7]
  Y. Jin, Q. Xue, D. He, X. Liu, S. Lu, J. Nanosci. Nanotechnol. 17 (2017) 6216-6223. doi: 10.1166/jnn.2017.14434
8. [8]
  H.S. Han, M.S. Ahmed, H. Jeong, S. Jeon, J. Electrochem. Soc. 162 (2015) B75-B80. doi: 10.1149/2.0651504jes
9. [9]
  W. Wang, L. Liu, L. Xu, et al., Part. Part. Syst. Charact. 33 (2016) 388-395. doi: 10.1002/ppsc.201500219
10. [10]
  Y. Huang, J. Tan, L.J. Cui, et al., Biosens. Bioelectron. 102 (2018) 560-567. doi: 10.1016/j.bios.2017.11.037
11. [11]
  R. Li, X. Zhang, C. Yang, et al., J. Nanosci. Nanotechnol. 18 (2018) 3800-3805. doi: 10.1166/jnn.2018.15257
12. [12]
  F. Chen, X. Zhang, X. Hu, P. Liu, H. Zhang, Nanosci. Nanotechnol. Lett. 10 (2018) 259-266. doi: 10.1166/nnl.2018.2621
13. [13]
  J. Yang, F. Nian, Z. Guo, T. Bai, G. Peng, Nanosci. Nanotechnol. Lett. 9 (2017) 1491-1496. doi: 10.1166/nnl.2017.2503
14. [14]
  Y. Xu, Y. Liu, Y. Wu, et al., Anal. Chem. 86 (2014) 5611-5614. doi: 10.1021/ac5010458
15. [15]
  S. Takalkar, K. Baryeh, G. Liu, Biosens. Bioelectron. 98 (2017) 147-154. doi: 10.1016/j.bios.2017.06.045
16. [16]
  L.L. Cao, Y. Jiang, Z. Chen, J. Nanosci. Nanotechnol. 18 (2018) 3067-3076. doi: 10.1166/jnn.2018.14674
17. [17]
  F. Zhao, L. Cao, Y. Liang, et al., J. Biomed. Nanotechnol. 13 (2017) 1300-1308. doi: 10.1166/jbn.2017.2415
18. [18]
  Y. Xing, X.Z. Feng, L.P. Zhang, et al., Int. J. Nanomed. Nanosurg. 12 (2017) 3171-3179. doi: 10.2147/IJN
19. [19]
  Y. Wu, W. Peng, Q. Zhao, et al., Chin. Chem. Lett. 28 (2017) 1881-1884. doi: 10.1016/j.cclet.2017.07.026
20. [20]
  M. Zarei, Biosens. Bioelectron. 98 (2017) 494-506. doi: 10.1016/j.bios.2017.07.024
21. [21]
  X. Gong, J. Cai, B. Zhang, et al., J. Mater. Chem. B -Mater. Biol. Med. 5 (2017) 5079-5091. doi: 10.1039/C7TB01049D
22. [22]
  M. Liu, K. Wu, L. Zhao, Y. Zhang, J. Biomed. Nanotechnol. 13 (2017) 381-392. doi: 10.1166/jbn.2017.2354
23. [23]
  J. Fan, X. Yu, L. He, et al., Nanosci. Nanotechnol. Lett. 9 (2017) 1478-1486. doi: 10.1166/nnl.2017.2501
24. [24]
  H. Li, X.M. Xie, Chin. Chem. Lett. 29 (2018) 161-165. doi: 10.1016/j.cclet.2017.06.001
25. [25]
  Standards of the Pharmaceutical Industry of the People's Republic of China, China Standards Press, 2018(YY/T 1605-2018).
26. [26]
  D. Zhang, L. Huang, B. Liu, et al., Biosens. Bioelectron. 106 (2018) 204-211. doi: 10.1016/j.bios.2018.01.062
27. [27]
  D. Zhang, L. Huang, B. Liu, et al., Sensor. Actuat. B -Chem. 277 (2018) 502-509. doi: 10.1016/j.snb.2018.09.044
28. [28]
  Y. Du, L. Wang, W. Wang, et al., J. Biomed. Nanotechnol. 14 (1) (2018) 161-167.
29. [29]
  L. Li, G. Rao, X. Lv, et al., J. Biomed. Opt. 23 (2018) 1-4.
30. [30]
  S. Ma, L. Li, M. She, et al., Chin. Chem. Lett. 28 (2017) 2014-2018. doi: 10.1016/j.cclet.2017.09.027
31. [31]
  S. Song, N. Liu, Z. Zhao, et al., Anal. Chem. 86 (2014) 4995-5001. doi: 10.1021/ac500540z
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