Citation: Liu Dailei, Wan Bo, Qi Jianping, Dong Xiaochun, Zhao Weili, Wu Wei, Dai Yikang, Lu Yi, Chen Zhongjian. Permeation into but not across the cornea: Bioimaging of intact nanoemulsions and nanosuspensions using aggregation-caused quenching probes[J]. Chinese Chemical Letters, ;2018, 29(12): 1834-1838. doi: 10.1016/j.cclet.2018.11.015 shu

Permeation into but not across the cornea: Bioimaging of intact nanoemulsions and nanosuspensions using aggregation-caused quenching probes

Liu Dailei ^a ,
Wan Bo ^a ,
Qi Jianping ^a,b ,
Dong Xiaochun ^a ,
Zhao Weili ^a,b ,
Wu Wei ^a,b ,
Dai Yikang ^c,* ,
Lu Yi ^a,b,** ,
Chen Zhongjian ^b,*

a.
School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery of MOE and PLA, Shanghai 201203, China
b.
Shanghai Dermatology Hospital, Shanghai 200443, China
c.
Department of Ophthalmology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China

kangkang56@126.com

fd_luyi@fudan.edu.cn

aajian818@163.com

Received Date: 30 September 2018
Revised Date: 25 October 2018
Accepted Date: 12 November 2018
Available Online: 14 December 2018

Figures(4)

Nanoemulsions (NEs) and nanosuspensions (NSs) show great potential in enhancing the ocular bioavailability of therapeutics through topical delivery. However, transocular fate of intact NEs and NSs is still inconclusive. In this study, an aggregation-caused quenching fluorescent probe is used to track precorneal retention and transocular transportation of intact NEs and NSs, while coumarin 6 is used to mimick the cargo. NEs show superior precorneal retention to NSs. Both the two types of nanocarriers can permeate into but not across the cornea. The smaller NEs (100 nm) permeate better into the cornea than the bigger ones (210 nm). Nanocarriers in the cornea serves as depots. The released cargo molecules can penetrate across the cornea and diffuse into the lens. Moreover, the conjunctiva-scleral route may be potential to deliver drugs to the back of the eye. In conclusion, the study provides useful tools and information in the field of transocular transportation of nanoparticles.
- Transocular transportation,
- Topical administration,
- Aggregation-caused quenching probe,
- Nanoemulsions,
- Nanosuspensions
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