Citation: Liu Chang, Shen Han, Wang Su, Cao Xiaoli, Xu Hongpan, Xia Yanyan, Bai Tingting, Liu Yufeng, Peng Lijun, Li Chuchu, Guo Zhirui, Li Zhiyang. Spermine increases bactericidal activity of silver-nanoparticles against clinical methicillin-resistant Staphylococcus aureus[J]. Chinese Chemical Letters, ;2018, 29(12): 1824-1828. doi: 10.1016/j.cclet.2018.10.025 shu

Spermine increases bactericidal activity of silver-nanoparticles against clinical methicillin-resistant Staphylococcus aureus

Liu Chang ^a,1 ,
Shen Han ^a,1 ,
Wang Su ^c ,
Cao Xiaoli ^a ,
Xu Hongpan ^a ,
Xia Yanyan ^a ,
Bai Tingting ^b ,
Liu Yufeng ^c ,
Peng Lijun ^a ,
Li Chuchu ^d ,
Guo Zhirui ^b,* ,
Li Zhiyang ^a,*

a.
Department of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
b.
Department of Geriatrics, The Second Affiliated Hospital, Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing 210011, China
c.
College of Life Science, Yangtze University, Jingzhou 434025, China
d.
Department of Technical Services, Jiangsu Provincial Center for Disease Prevention and Control, Nanjing 210009, China

zhiruiguo@njmu.edu.cn

lizhiyangcn@qq.com

Received Date: 29 September 2018
Revised Date: 18 October 2018
Accepted Date: 18 October 2018
Available Online: 24 December 2018

Figures(3)

Methicillin-resistant Staphylococcus aureus (MRSA) has emerged worldwide as a major multidrugresistant pathogen that causes notable morbidity and mortality. Fast emerging of MRSA prevalence requires special attention for strengthening the inventory of antimicrobial compounds. Silver nanoparticles (AgNPs) have been widely used to treat multi-drug resistant pathogens due to the unique antibacterial properties, meanwhile spermine has been proven to exert outstanding inhibition effect to S. aureus with not yet fully understood mechanisms. The aim of this study was to investigate the synergistic effect of AgNPs and spermine as well as to determine the antibacterial activity of their combination against MRSA strains. Several clinical MRSA isolates and ATCC BAA-1026 were used to determine minimum inhibitory concentration (MIC) and fractional inhibitory concentration indices (FICI) of AgNPs and spermine, and a synergistic effect was observed. This phenomenon was further confirmed by growth curve and time-killing assays, showed that spermine could be used as an adjuvant for AgNPs in the treatment of MRSA infections.
- Spermine,
- Silver nanoparticles,
- Staphylococcus aureus,
- Synagistic effect,
- Antimicrobial
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