Citation: Chu Guangcui, Cheng Dandan, Liu Wenjuan, Wang Xuefeng, Hou Xiaotao, Hou Yuanyuan, Wei Hongjun, Deng Jiagang, Bai Gang. Screening and evaluation of antioxidants from lees by micro-injector systems combined with a fluorescent probe, N-borylbenzyloxycarbonyl-3, 7-dihydroxyphenoxazine, in living Drosophila[J]. Chinese Chemical Letters, ;2018, 29(10): 1521-1527. doi: 10.1016/j.cclet.2018.05.020 shu

Screening and evaluation of antioxidants from lees by micro-injector systems combined with a fluorescent probe, N-borylbenzyloxycarbonyl-3, 7-dihydroxyphenoxazine, in living Drosophila

a.
Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
b.
State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300353, China
c.
Guangxi Collaborative Innovation Center of Study on Functional Ingredients of Agricultural Residues, Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning 530200, China
d.
JF-Pharmaceutical Technology Development Co., Ltd., Tianjin 300457, China

Corresponding author: Hou Yuanyuan, houyy@nankai.edu.cn Wei Hongjun, weihongjun@188.com
Received Date: 27 March 2018
Revised Date: 4 May 2018
Accepted Date: 7 May 2018
Available Online: 9 October 2018

Figures(4)

Over the past few decades, the determination of antioxidant activity by chemical probe has been widely reported, but in vivo evaluation via model organisms of Drosophila melanogaster and rapid discovery system has not been studied adequately. In this study, we determined the antioxidant activity of lees and demonstrated the ability of compounds in lees to scavenge H₂O₂ in vitro and in vivo by different chemical probes. Lees increased the ability of Drosophila against oxidative stress and antioxidant enzyme activity in vivo. Five ingredients of organic acids and flavones in lees extract that rapidly scavenged H₂O₂ were revealed by a post-column-derived HPLC-UV-FLD system based on 4-hydroxyphenylacetic acid (PHPAA) chemiluminescence. Additionally, another fluorescent probe, N-borylbenzyloxycarbonyl-3, 7-dihydroxyphenoxazine (NBCD), was selected to evaluate the reactive oxygen species (ROS) scavenging capacity of lees in living Drosophila melanogaster using a microfluidic injection test coupled with microscopic imaging analysis, and similar effects were observed in flies when they were treated with tartaric acid and caffeic acid. The results demonstrated that the novel integrated system was suitable for screening and evaluating antioxidant ingredients from natural products.
- Lees,
- Antioxidant,
- Microinjection,
- HPLC-UV-FLD,
- NBCD,
- PHPAA
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沈阳化工大学材料科学与工程学院沈阳 110142