Citation: Xia Ying, Liu Xiaoyu, Wang Dan, Wang Zechen, Liu Qiang, Yu Haibo, Zhang Mingyan, Song Youtao. A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples[J]. Chinese Chemical Letters, ;2018, 29(10): 1517-1520. doi: 10.1016/j.cclet.2018.01.054 shu

A fluorometric and mitochondrion-targetable probe for rapid, naked-eye test of hypochlorite in real samples

Xia Ying ^a,1 ,
Liu Xiaoyu ^a,1 ,
Wang Dan ^a ,
Wang Zechen ^a ,
Liu Qiang ^a ,
Yu Haibo ^a,, ,
Zhang Mingyan ^b,, ,
Song Youtao ^a,,

a.
College of Environmental Sciences, Liaoning University, Shenyang 110036, China
b.
Liaoning Center of Disease Prevention and Control, Shenyang 110001, China

Corresponding author: Yu Haibo, yuhaibo@lnu.edu.cn Zhang Mingyan, lncdczmy@163.com Song Youtao, youtaos@gmail.com

Received Date: 16 November 2017
Revised Date: 23 January 2018
Accepted Date: 29 January 2018
Available Online: 5 October 2018

Figures(4)

Hypochlorite anion is a ubiquitous reactive molecule in the terminal disinfection systems, inflammatory stress and immune systems. Thus, rapid and visual monitoring ClO^- in water and biological samples is very meaningful for water quality safety and toxicity assessment of contaminants. Herein, a colorimetric and fluorometric probe (Rh-ClO) based on rhodamine B fluorophore and thiophene-2-carbohydrazide has been unveiled and successfully utilized for ClO^- detection in water samples and HeLa cells. Upon addition of ClO^-, color changes of solution from colorless to pink were immediately visible to the nakedeyes, meanwhile, brilliant red fluorescence was observed under excited at UV light (365 nm). Rh-ClO displayed high selectivity and sensitivity for ClO^-, and the detection limit was 7 ǌmol/L calculated from the fluorescence titration. With the aid of its merits including rapid response to ClO^- within 10 s, Rh-ClO and its test paper could successfully detect ClO^- in water. Additionally, HeLa cells image co-stained with Rh-ClO and Rh123 demonstrated that Rh-ClO possessed excellent and fast cell-membrane permeability and mitochondrion-targetability. It was clearly confirmed that Rh-ClO would be a promising probe for rapid tracking of ClO^- in water samples and in mitochondria of living cells.
- Colorimetric probe,
- Fluorescent probe,
- Rhodamine,
- Hypochlorite,
- Mitochondrion-tragetable
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