Citation: Rui Yin, Wu Xiaomei, Ma Baodi, Xu Yi. Immobilization of acetylcholinesterase on functionalized SBA-15 mesoporous molecular sieve for detection of organophosphorus and carbamate pesticide[J]. Chinese Chemical Letters, ;2018, 29(9): 1387-1390. doi: 10.1016/j.cclet.2017.10.033 shu

Immobilization of acetylcholinesterase on functionalized SBA-15 mesoporous molecular sieve for detection of organophosphorus and carbamate pesticide

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Corresponding author: Xu Yi, xuyi@sit.edu.cn
Received Date: 24 May 2017
Revised Date: 23 October 2017
Accepted Date: 26 October 2017
Available Online: 27 September 2017

Figures(2)

The immobilization of acetylcholinesterase (AChE) on amino functionalized SBA-15 mesoporous sieves (NH₂-SBA-15) was explored for detection of organophosphorus and carbamate pesticides. Several methods have been studied and the "adsorption-crosslinking" is found to be the best one. Firstly, AChE and bovine serum albumin (BSA) were adsorbed in turn on the surface of NH₂-SBA-15 by electrostatic adsorption, and then glutaraldehyde was added for cross-linking the amino group of AChE. The NH₂-SBA-15 showed around 95% immobilization efficiency and the specific activity of immobilized AChE (AChENH₂-SBA-15) reached 130% relative to free AChE under optimal immobilization conditions. It was found AChE-NH₂-SBA-15 retained 94.0% and 82.8% of its initial activity after 60 days at 4℃ and 25℃, respectively. It remained 55.0% of its initial activity after 6 times recycling. During the detection of pesticide, the AChE-NH₂-SBA-15 showed wider linear range and much lower limit of detection compared with free AChE. The current method showed good recovery (93.8%-109.3%) and low RSD (< 5%) for both standard solution and real vegetable samples of Carbaryl and Trichlorfon. It was believed that AChE-NH₂-SBA-15 could be exploited as a fast, sensitive and low-cost biocatalyst towards the detection of pesticides residues which could be stored at room temperature for a long time.
- Acetylcholinesterase,
- Immobilization,
- Adsorption-crosslinking,
- Mesoporous SBA-15 molecular sieve,
- Pesticide residues detection
1. [1]
  A. Crew, D. Lonsdale, N. Byrd, R. Pittson, J.P. Hart, Biosens. Bioelectron. 26(2011) 2847-2851. doi: 10.1016/j.bios.2010.11.018
2. [2]
  X. Sun, X.Y. Wang, Biosens. Bioelectron. 25(2010) 2611-2614. doi: 10.1016/j.bios.2010.04.028
3. [3]
  Y.Q. Miao, N.Y. He, J.J. Zhu, Chem. Rev. 110(2010) 5216-5234. doi: 10.1021/cr900214c
4. [4]
  R.B. Dominguez, G.A. Alonso, R. Muñoz, A. Hayzt, J. Marty, Sensor. Actuat. BChem. 208(2015) 491-496. doi: 10.1016/j.snb.2014.11.069
5. [5]
  Z.M. Liu, Y.F. Jing, Z.L. Wang, H.J. Zhan, Q. Shen, Sensor. Lett. 11(2013) 531-538. doi: 10.1166/sl.2013.2762
6. [6]
  M.M. Liang, K.L. Fan, Y. Pan, et al., Anal. Chem. 85(2013) 308-312. doi: 10.1021/ac302781r
7. [7]
  Y.D. Zhang, Y.N. Cai, Z.L. Qi, L. Lu, Y.X. Qian, Anal. Chem. 85(2013) 8455-8461. doi: 10.1021/ac401966d
8. [8]
  H.C. Li, Y.X. Guo, L.H. Xiao, B. Chen, Analyst 139(2014) 285-289. doi: 10.1039/C3AN01736B
9. [9]
  J.D. Keighron, S. Akesson, A.S. Cans, Langmuir 30(2014) 11348-11355. doi: 10.1021/la502538h
10. [10]
  D.B. Liu, W.W. Chen, J.H. Wei, et al., Anal. Chem. 84(2012) 4185-4191. doi: 10.1021/ac300545p
11. [11]
  C.P. Li, S.M. Fan, C.Y. Yin, et al., Anal. Methods 6(2014) 1914-1921. doi: 10.1039/C3AY42305K
12. [12]
  D.N. Kumar, S.A. Alex, N. Chandrasekaran, A. Mukherjee, Rsc. Adv. 6(2016) 64769-64777. doi: 10.1039/C6RA13185A
13. [13]
  L.T. Zhang, J. Chen, Y.T. Wang, et al., Sensor. Actuat. B-Chem. 193(2014) 904-910. doi: 10.1016/j.snb.2013.11.092
14. [14]
  S.P. Zhang, L.G. Shan, Z.R. Tian, et al., Chin. Chem. Lett. 19(2008) 592-594. doi: 10.1016/j.cclet.2008.03.014
15. [15]
  F.F. Huang, J.M. Wang, S. Wu, Y.F. Zhang, T. Yang, Sci. Adv. Mater. 5(2013) 1097-1104. doi: 10.1166/sam.2013.1559
16. [16]
  X.S. Guo, X.Y. Zhang, Q. Cai, T. Shen, S.M. Zhu, Food. Control. 30(2013) 15-23. doi: 10.1016/j.foodcont.2012.07.015
17. [17]
  T. Shimomura, T. Itoh, T. Sumiya, F. Mizukami, M. Ono, Enzyme. Microb. Technol. 45(2009) 443-448. doi: 10.1016/j.enzmictec.2009.08.007
18. [18]
  R.K. Mishra, R.B. Dominguez, S. Bhand, R. Muñoz, J.L. Marty, Biosens. Bioelectron. 32(2012) 56-61. doi: 10.1016/j.bios.2011.11.028
19. [19]
  H.P. Mao, Y.T. Yan, N. Hao, et al., Sensor. Actuat. B-Chem. 238(2017) 239-248. doi: 10.1016/j.snb.2016.07.072
20. [20]
  I. Kucherenko, O. Soldatkin, B.O. Kasap, et al., Nanoscale. Res. Lett. 10(2015) 209-217. doi: 10.1186/s11671-015-0911-6
21. [21]
  J. Fan, C.Z. Yu, F. Gao, et al., Angew. Chem. Int. Ed. 42(2003) 3146-3150. doi: 10.1002/anie.200351027
22. [22]
  J.L. Blin, C. Cérardin, C. Carteret, et al., Chem. Mater. 17(2005) 1479-1486. doi: 10.1021/cm048033r
23. [23]
  T. Itoh, R. Ishii, T. Hanaoka, et al., J. Mol. Catal. B-Enzym. 57(2009) 183-187. doi: 10.1016/j.molcatb.2008.08.014
24. [24]
  G.R. Bardajee, M. Mohammadi, H. Yari, A. Ghaedi, Chin. Chem. Lett. 27(2016) 265-270. doi: 10.1016/j.cclet.2015.10.011
25. [25]
  X.Y. Sun, P.Z. Li, B. Ai, Y.B. Wang, Chin. Chem. Lett. 27(2016) 139-144. doi: 10.1016/j.cclet.2015.08.008
26. [26]
  H. Takahashi, B. Li, T. Sasaki, et al., Chem. Mater. 12(2000) 3301-3305. doi: 10.1021/cm000487a
27. [27]
  Z.X. Ao, J.H. Hui, W.X. Feng, et al., Chin. Sci. Bull. 54(2009) 3023-3028. doi: 10.1007/s11434-009-0441-7
28. [28]
  M.M. Wan, W.G. Lin, L. Gao, H.C. Gu, J.H. Zhu, J. Colloid. Interf. Sci. 377(2012) 497-503. doi: 10.1016/j.jcis.2012.03.057
29. [29]
  C.H. Lei, Y. Shin, J. Liu, E.J. Ackerman, J. Am. Chem. Soc. 124(2002) 11242-11243. doi: 10.1021/ja026855o
30. [30]
  H.H.P. Yiu, P.A. Wright, N.P. Botting, J. Mol. Catal. B-Enzym. 15(2001) 81-92. doi: 10.1016/S1381-1177(01)00011-X
31. [31]
  I. Matlahov, Y. Geiger, G. Goobes, Phys. Chem. Chem. Phys. 16(2014) 9031-9038. doi: 10.1039/C3CP55520H
32. [32]
  J.B. Kim, J.W. Grate, P. Wang, Chem. Eng. Sci. 61(2006) 1017-1026. doi: 10.1016/j.ces.2005.05.067
33. [33]
  D.Y. Zhao, P.D. Yang, N. Melosh, et al., Adv. Mater. 10(1998) 1380-1385. doi: 10.1002/(ISSN)1521-4095
34. [34]
  Q.Q. Zhang, Q.C. Xu, Y.M. Guo, X. Sun, X.Y. Wang, RSC Adv. 6(2016) 24698-24703. doi: 10.1039/C5RA21799G
35. [35]
  H.D. Zhang, Y. Zou, Y. Shen, et al., Bioenerg. Res. 7(2014) 1541-1549. doi: 10.1007/s12155-014-9492-y
36. [36]
  M.C.R. Hernández, J.E.M. Wejebe, J.C. Basurto, et al., Int. J. Bio. Macromol. 40(2007) 444-448. doi: 10.1016/j.ijbiomac.2006.11.004
37. [37]
  K. Kannan, R.V. Jasra, J. Mol. Catal. B-Enzym. 56(2009) 34-40. doi: 10.1016/j.molcatb.2008.04.007
38. [38]
  A.S.M. Chong, X.S. Zhao, J. Phys. Chem. B 107(2003) 12650-12657. doi: 10.1021/jp035877+
39. [39]
  A.V. Singh, B.M. Bandgar, M. Kasture, B.L.V. Prasad, M. Sastry, J. Mater. Chem. 15(2005) 5115-5121. doi: 10.1039/b510398c
40. [40]
  M. Saleem, M. Rafiq, S.Y. Seo, K.H. Lee, Biosci. Rep. 36(2016) e00311. doi: 10.1042/BSR20150154
41. [41]
  T. Itoh, T. Shimomura, A. Hayashi, et al., Analyst 139(2014) 4654-4660. doi: 10.1039/C4AN00975D
42. [42]
  H. Tümtürk, F. Sahin, G. Demirel, Bioproc. Biosyst. Eng. 30(2007) 141-145. doi: 10.1007/s00449-006-0106-8
43. [43]
  J. Marty, K. Sode, I. Karube, Electroanalytical 4(1992) 249-252. doi: 10.1002/(ISSN)1521-4109
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