Citation: Rui Yin, Wu Xiaomei, Ma Baodi, Xu Yi. Immobilization of acetylcholinesterase on functionalized SBA-15 mesoporous molecular sieve for detection of organophosphorus and carbamate pesticide[J]. Chinese Chemical Letters, ;2018, 29(9): 1387-1390. doi: 10.1016/j.cclet.2017.10.033 shu

Immobilization of acetylcholinesterase on functionalized SBA-15 mesoporous molecular sieve for detection of organophosphorus and carbamate pesticide

School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China

Corresponding author: Xu Yi, xuyi@sit.edu.cn
Received Date: 24 May 2017
Revised Date: 23 October 2017
Accepted Date: 26 October 2017
Available Online: 27 September 2017

Figures(2)

The immobilization of acetylcholinesterase (AChE) on amino functionalized SBA-15 mesoporous sieves (NH₂-SBA-15) was explored for detection of organophosphorus and carbamate pesticides. Several methods have been studied and the "adsorption-crosslinking" is found to be the best one. Firstly, AChE and bovine serum albumin (BSA) were adsorbed in turn on the surface of NH₂-SBA-15 by electrostatic adsorption, and then glutaraldehyde was added for cross-linking the amino group of AChE. The NH₂-SBA-15 showed around 95% immobilization efficiency and the specific activity of immobilized AChE (AChENH₂-SBA-15) reached 130% relative to free AChE under optimal immobilization conditions. It was found AChE-NH₂-SBA-15 retained 94.0% and 82.8% of its initial activity after 60 days at 4℃ and 25℃, respectively. It remained 55.0% of its initial activity after 6 times recycling. During the detection of pesticide, the AChE-NH₂-SBA-15 showed wider linear range and much lower limit of detection compared with free AChE. The current method showed good recovery (93.8%-109.3%) and low RSD (< 5%) for both standard solution and real vegetable samples of Carbaryl and Trichlorfon. It was believed that AChE-NH₂-SBA-15 could be exploited as a fast, sensitive and low-cost biocatalyst towards the detection of pesticides residues which could be stored at room temperature for a long time.
- Acetylcholinesterase,
- Immobilization,
- Adsorption-crosslinking,
- Mesoporous SBA-15 molecular sieve,
- Pesticide residues detection
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