Citation: Liu Zheyi, Jiang You, Xiao Chunlei, Xiong Xingchuang, Wang Tao, Li Jinan, Wang Fangjun, Fang Xiang, Yang Xueming. Elucidating the various multi-phosphorylation statuses of protein functional regions by 193-nm ultraviolet photodissociation[J]. Chinese Chemical Letters, ;2018, 29(5): 694-698. doi: 10.1016/j.cclet.2017.10.020 shu

Elucidating the various multi-phosphorylation statuses of protein functional regions by 193-nm ultraviolet photodissociation

Liu Zheyi ^a,1 ,
Jiang You ^b,1 ,
Xiao Chunlei ^c,, ,
Xiong Xingchuang ^b ,
Wang Tao ^c ,
Li Jinan ^a ,
Wang Fangjun ^a,, ,
Fang Xiang ^b,, ,
Yang Xueming ^c

a.
CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
National Institute of Metrology, Beijing 100013, China
c.
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: Xiao Chunlei, chunleixiao@dicp.ac.cn Wang Fangjun, wangfj@dicp.ac.cn Fang Xiang, fangxiang@nim.ac.cn

Received Date: 22 June 2017
Revised Date: 25 September 2017
Accepted Date: 16 October 2017
Available Online: 18 May 2017

Figures(4)

Ultraviolet photodissociation is a high-energy fast excitation method in mass spectrometry and has been successfully applied for the elucidation of sequences and structures of biomolecules. However, its ability to distinguish the phosphorylation sites isomers of multi-phosphopeptides has been not systematically investigated until now. A 193-nm ultraviolet laser dissociation mass spectrometry system was established in this study and applied to elucidate the complex multi-phosphorylation statuses mimicking the functional regions of Sic1, Gli3 and Tau. The numbers of matched fragment ions and phosphorylation site-determining ions were improved on average 123% and 104%, respectively, by utilizing the ultraviolet photodissociation strategy, comparing to the typically utilized collision induced dissociation strategy. Finally, 94% phosphorylation sites within various statuses were unambiguously elucidated.
- Ultraviolet photodissociation,
- Mass spectrometry,
- Multi-phosphorylation,
- Phosphorylated tyrosine,
- Phosphorylation site-determining ion
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