Citation: Wang Ning, Li Sheng-Tao, Lu Tian-Tian, Nakanishi Hideki, Gao Xiao-Dong. Approaches towards the core pentasaccharide in N-linked glycans[J]. Chinese Chemical Letters, ;2018, 29(1): 35-39. doi: 10.1016/j.cclet.2017.09.044 shu

Approaches towards the core pentasaccharide in N-linked glycans

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China

Author Bio:

Xiao-Dong Gao received his Ph.D. degree in 1994 at the Graduate School of Agricultural and Life Science, University of Tokyo, and then joined the laboratory of Prof. Yoshifumi Jigami as a postdoctoral fellow at National Institute of Bioscience and Human Technology. From 1998 to 2002, he worked as a research scientist in the laboratory of Prof. Neta Dean at State University of New York at Stony Brook, USA, and became an assistant professor in 2002. At 2003, he moved to Research Institute for Glycoscience, AIST as a senior research scientist, and then to Hokkaido University as an associate professor at 2006. In 2011, he returned to China, and now is working as the director of the Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University at Wuxi. His general research interests are to understand the glycosylation regulation and the biological roles of carbohydrate modifications on the cell surface, focusing on the biosynthesis pathway of N-linked glycosylation and the chemo-enzymatic assembling of N-glycans.
Corresponding author: Gao Xiao-Dong, xdgao@jiangnan.edu.cn
Received Date: 30 June 2017
Revised Date: 7 August 2017
Accepted Date: 21 September 2017
Available Online: 22 January 2017

Figures(5)

All eukaryotic cells share a common core pentasaccharide, Man3GlcNAc2 (M3), which is found in both dolichol-linked oligosaccharides (DLOs) and N-linked glycoproteins. A pentasaccharide M3 can be elongated and elaborated by various glycosyltransferases and is thus considered as the key intermediate in the in vitro synthetic pathway of N-glycans. In this review, we describe the progresses in the preparation of M3 by chemical and chemoenzymatic methods. We first draw a brief schematic view of the typical N-glycan biological pathway and then focus on the requirements and challenges in obtaining core pentasaccharides. Representative methods and recent reported findings, especially research progress in chemoenzymatic synthesis, are highlighted. In addition, the opportunities in exploiting novel methods for constructing N-glycans, as well as their applications, are discussed.
- N-Glycan,
- Pentasaccharide,
- Chemoenzymatic synthesis,
- Alg proteins,
- Mannosyltransferase
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