Citation: Sun Fei, Zhang Wen-Bin. Unleashing chemical power from protein sequence space toward genetically encoded "click" chemistry[J]. Chinese Chemical Letters, ;2017, 28(11): 2078-2084. doi: 10.1016/j.cclet.2017.08.052 shu

Unleashing chemical power from protein sequence space toward genetically encoded "click" chemistry

Sun Fei ^a,, ,
Zhang Wen-Bin ^b,,

a.
Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Hong Kong SAR, China
b.
Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Center for Soft Matter Science and Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China

Corresponding author: Sun Fei, kefsun@ust.hk Zhang Wen-Bin, wenbin@pku.edu.cn
Received Date: 5 July 2017
Revised Date: 9 August 2017
Accepted Date: 6 September 2017
Available Online: 6 November 2017

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Synthesis of macromolecular systems with precise structural and functional control constitutes a fundamental challenge for materials science and engineering. Development of the ability to construct complex bio-macromolecular architectures provides a solution to this challenge. The past few years have witnessed the emergence of a new category of peptide-protein chemistry which can covalently stitch together protein/peptide molecules with high specificity under mild physiological conditions. It has thus inspired the concept of genetically encoded click chemistry (GECC). As a prototype of GECC, SpyTag/SpyCatcher chemistry has enabled the precise synthesis ofmacromolecules both in vitro and in vivo, exerting precise control over the fundamental properties of these macromolecules including length, sequence, stereochemistry and topology and leading to the creation of diverse biomaterials for a variety of applications. We thus anticipate a potential toolbox of GECC comprising multiple mutually orthogonal, covalent-bond forming peptide-protein reactive pairs with diverse features, which shall bridge synthetic biology and materials science and open up enormous opportunities for biomaterialsin the future.
- Genetically encoded click chemistry,
- SpyTag,
- SpyCatcher,
- Topology,
- Protein engineering
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