Citation: Gao Cunji, Zhu Hongmei, Chen Jia, Qiu Hongdeng. Facile synthesis of enzyme functional metal-organic framework for colorimetric detecting H₂O₂ and ascorbic acid[J]. Chinese Chemical Letters, ;2017, 28(5): 1006-1012. doi: 10.1016/j.cclet.2017.02.011 shu

Facile synthesis of enzyme functional metal-organic framework for colorimetric detecting H₂O₂ and ascorbic acid

a.
Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
b.
Institute of Microbiology and Biochemical Pharmacy, School of Pharmacy, Lanzhou University, Lanzhou 730000, China

Corresponding author: Qiu Hongdeng, hdqiu@licp.cas.cn
Received Date: 4 November 2016
Revised Date: 30 December 2016
Accepted Date: 17 February 2017
Available Online: 6 May 2017

Figures(7)

In this work, a metal-organic frameworks material MIL-88 was prepared easily using solvent-thermal method, and was first found to have catalytic activities similar to those of biological enzymes such as catalase and peroxidase. The material was characterized by XRD, SEM, TEM, EDX, FT-IR techniques and an N₂ adsorption method. It exhibited peroxidase-like activity through catalytic oxidation of the peroxidase substrate 3,3', 5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂, producing a blue-colored solution. Under optimal conditions, the absorbance at 652 nm is linearly correlated with the concentration of H₂O₂ from 2.0×10^-6 mol/L to 2.03×10^-5 mol/L (R²=0.981) with a detection limit of 5.62×10^-7 mol/L (S/N=3). More importantly, a sensitive and selective method for ascorbic acid detection was developed using this material as a catalyst. The analytical method for ascorbic acid detection was observed to have a linear range from 2.57×10^-6 mol/L to 1.01×10^-5 mol/L (R²=0.989) with a detection limit of 1.03×10^-6 mol/L (S/N=3). This work suggests MOFs have advantages of preparing biomimetic catalysts and extends applications of the functional MOFs in the field of biosensor.
- Metal-organic frameworks,
- Horseradish peroxidase,
- H₂O₂,
- Ascorbic acid,
- Colorimetric detection,
- Biomimetic catalyst
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Facile synthesis of enzyme functional metal-organic framework for colorimetric detecting H2O2 and ascorbic acid

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通讯作者: 陈斌, bchen63@163.com

Facile synthesis of enzyme functional metal-organic framework for colorimetric detecting H₂O₂ and ascorbic acid