Citation: Zhao Chun-Ling, Hua Mei, Yang Can-Yu, Yang Yun-Hui. A novel aptasensor based on 3D-inorganic hybrid composite as immobilized substrate for sensitive detection of platelet-derived growth factor[J]. Chinese Chemical Letters, ;2017, 28(7): 1417-1423. doi: 10.1016/j.cclet.2017.02.010 shu

A novel aptasensor based on 3D-inorganic hybrid composite as immobilized substrate for sensitive detection of platelet-derived growth factor

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China

Corresponding author: Yang Yun-Hui, yyhui2002@aliyun.com
Received Date: 9 November 2016
Revised Date: 14 January 2017
Accepted Date: 6 February 2017
Available Online: 24 July 2017

Figures(7)

A novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure is reported in this paper. 3D-4MgCO₃·Mg(OH)₂·4H₂O-Au NPs inorganic hybrid composite was utilized as immobilized substrate for sensitive PDGF detection and Pt-Au bimetallic nanoparticles were labelled on PDGF aptamer to indirectly detect PDGF for the first time. The proposed aptasensor exhibited a high catalytic efficiency towards reduction of H₂O₂, hence the sensitive detection of PDGF was achieved. Results showed that the aptasensor exhibited excellent linear response to PDGF, in the range of 0.1 pg/mL-10 ng/mL (4 fmol/L-400 pmol/L), with detection limit of 0.03 pg/mL (1.2 fmol/L).
- Aptasensor,
- Platelet-derived growth factor,
- 3D-inorganic hybrid composite,
- Pt-Au bimetallic nanoparticles,
- 4MgCO₃·Mg(OH)₂·4H₂O-Au NPs
1. [1]
  Sun Y.H., Cai S., Lau C.W., Zhu J.H., Lu J.Z.. Novel PDGF aptasensor based on gold nanoparticle triggered chemiluminescence[J]. Procedia Eng., 2011,25:1565-1568. doi: 10.1016/j.proeng.2011.12.387
2. [2]
  Bunka D.H.J., Stockley P.G.. Aptamers come of age—at last[J]. Nat. Rev. Microbiol., 2006,4:588-596. doi: 10.1038/nrmicro1458
3. [3]
  Willner I., Zayats M.. Electronic aptamer-based sensors[J]. Angew. Chem. Int. Ed., 2007,46:6408-6418. doi: 10.1002/(ISSN)1521-3773
4. [4]
  Ellington A.D., Szostak J.W.. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990,346:818-822. doi: 10.1038/346818a0
5. [5]
  Tuerk C., Gold L.. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990,249:505-510. doi: 10.1126/science.2200121
6. [6]
  Jayasena S.D.. Aptamers: an emerging class of molecules that rival antibodies in diagnostics[J]. Clin. Chem., 1999,45:1628-1650.
7. [7]
  Patel D.J., Suri A.K.. Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics[J]. Rev. Mol. Biotechnol., 2000,74:39-60. doi: 10.1016/S1389-0352(99)00003-3
8. [8]
  Clark S.L., Remcho V.T.. Aptamers as analytical reagents[J]. Electrophoresis, 2002,23:1335-1340. doi: 10.1002/(ISSN)1522-2683
9. [9]
  Luzi E., Minunni M., Tombelli S., Mascini M.. New trends in affinity sensing: aptamers for ligand binding[J]. TrAC Trends. Anal. Chem., 2003,22:810-818. doi: 10.1016/S0165-9936(03)01208-1
10. [10]
  You K.M., Lee S.H., Im A., Lee S.B.. Aptamers as functional nucleic acids: in vitro selection and biotechnological applications[J]. Biotechnol. Bioprocess Eng., 2003,8:64-75. doi: 10.1007/BF02940259
11. [11]
  Huang C.C., Huang Y.F., Cao Z.H., Tan W.H., Chang H.T.. Aptamer-modified gold nanoparticles for colorimetric determination of platelet-derived growth factors and their receptors[J]. Anal. Chem., 2005,77:5735-5741. doi: 10.1021/ac050957q
12. [12]
  Huang B., Tong F.Y., Chen Y.T.. Fabrication and bioproperties of raspberrytype hybrid nanoparticles of Au-thioethyl pendant Ligand@Chitosan[J]. J. Biomed. Nanotechnol., 2013,9:115-123. doi: 10.1166/jbn.2013.1476
13. [13]
  Leng C., Lai G.S., Yan F., Ju H.X.. Gold nanoparticle as an electrochemical label for inherently crosstalk-free multiplexed immunoassay on a disposable chip[J]. Anal. Chim. Acta, 2010,666:97-101. doi: 10.1016/j.aca.2010.03.060
14. [14]
  Katiyar N., Selvakumar L.S., Patra S., Thaku M.S.. Gold nanoparticles based colorimetric aptasensor for theophylline[J]. Anal. Methods, 2013,5:653-659. doi: 10.1039/C2AY26133B
15. [15]
  Tang D.P., Ren J.J.. In situ amplified electrochemical immunoassay for carcinoembryonic antigen using horseradish peroxidase-encapsulated nanogold hollow microspheres as labels[J]. Anal. Chem., 2008,80:8064-8070. doi: 10.1021/ac801091j
16. [16]
  Wiglusz R.J., Kedziora A., Lukowiak A., Doroszkiewicz W., Strek W.. Hydroxyapatites and europium(Ⅲ) doped hydroxyapatites as a carrier of silver nanoparticles and their antimicrobial activity[J]. J. Biomed. Nanotechnol., 2012,8:605-612. doi: 10.1166/jbn.2012.1424
17. [17]
  Li F., Feng Y., Wang Z.. Direct electrochemistry of horseradish peroxidase immobilized on the layered calcium carbonate?gold nanoparticles inorganic hybrid composite[J]. Biosens. Bioelectron., 2010,25:2244-2248. doi: 10.1016/j.bios.2010.03.006
18. [18]
  Cai W.Y., Xu Q., Zhao X.N., Zhu J.J., Chen H.Y.. Porous gold-nanoparticle-CaCO₃ hybrid material: preparation, characterization, and application for horseradish peroxidase assembly and direct electrochemistry[J]. Chem. Mater., 2006,18:279-284. doi: 10.1021/cm051442i
19. [19]
  Song X.F., Yang C., Wang J., Sun S.Y., Yu J.G.. Synthesis of porous hydromagnesite microspheres with rosette-like morphology[J]. Chin. J. Inorg. Chem., 2011,27:1008-1014.
20. [20]
  Zhang H., Yin Y.J., Hu Y.J.. Pd@Pt core-shell nanostructures with controllable composition synthesized by a microwave method and their enhanced electrocatalytic activity toward oxygen reduction and methanol oxidation[J]. J. Phys. Chem. C, 2010,114:11861-11867.
21. [21]
  Fu X.M., Liu Z.J., Cai S.X.. Electrochemical aptasensor for the detection of vascular endothelial growth factor (VEGF) based on DNA-templated Ag/Pt bimetallic nanoclusters[J]. Chin. Chem. Lett., 2016,27:920-926. doi: 10.1016/j.cclet.2016.04.014
22. [22]
  Degefa T.H., Kwak J.. Label-free aptasensor for platelet-derived growth factor (PDGF) protein[J]. Anal. Chim. Acta., 2008,613:163-168. doi: 10.1016/j.aca.2008.03.010
23. [23]
  Lai R.Y., Plaxco K.W., Heeger A.J.. Aptamer-based electrochemical detection of picomolar platelet-derived growth factor directly in blood serum[J]. Anal. Chem., 2007,79:229-233. doi: 10.1021/ac061592s
24. [24]
  Zhou L., Ou L.J., Chu X., Shen G.L., Yu R.Q.. Aptamer-based rolling circle amplification: a platform for electrochemical detection of protein[J]. Anal. Chem., 2007,79:7492-7500. doi: 10.1021/ac071059s
25. [25]
  Zhang Y.L., Huang Y., Jiang J.H., Shen G.L., Yu R.Q.. Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step reusable, sensitive protein detection[J]. J. Am. Chem. Soc., 2007,129:15448-15449. doi: 10.1021/ja0773047
26. [26]
  Wang J., Meng W.Y., Zheng X.F., Liu S.L., Li G.X.. Combination of aptamer with gold nanoparticles for electrochemical signal amplification: application to sensitive detection of platelet-derived growth factor[J]. Biosens. Bioelectron., 2009,24:1598-1602. doi: 10.1016/j.bios.2008.08.030
27. [27]
  Deng K., Xiang Y., Zhang L.Q., Chen Q.H., Fu W.L.. An aptamer-based biosensing platform for highly sensitive detection of platelet-derived growth factor via enzyme-mediated direct electrochemistry[J]. Anal. Chim. Acta., 2013,759:61-65. doi: 10.1016/j.aca.2012.11.018
28. [28]
  Bai L.J., Yuan R., Chai Y.Q.. Simultaneous electrochemical detection of multiple analytes based on dual signal amplification of single-walled carbon nanotubes and multi-labeled graphene sheets[J]. Biomaterials, 2012,33:1090-1096. doi: 10.1016/j.biomaterials.2011.10.012
29. [29]
  Chai Y., Tian D.Y., Gu J., Cui H.. A novel electrochemiluminescence aptasensor for protein based on a sensitive N-(aminobutyl)-N-ethylisoluminol-functionalized gold nanoprobe[J]. Analyst., 2011,136:3244-3251. doi: 10.1039/c1an15298j
30. [30]
  Yang X.H., Sun S., Liu P.. A novel fluorescent detection for PDGF-BB based on dsDNA-templated copper nanoparticles[J]. Chin. Chem. Lett., 2014,25:9-14. doi: 10.1016/j.cclet.2013.10.032
31. [31]
  Huang K.J., Liu Y.J., Zhai Q.F.. Ultrasensitive biosensing platform based on layered vanadium disulfide-graphene composites coupling with tetrahedronstructured DNA probes and exonuclease Ⅲ assisted signal amplification[J]. J. Mater. Chem. B, 2015,3:8180-8187. doi: 10.1039/C5TB01239B
32. [32]
  Huang K.J., Shuai H.L., Zhang J.Z.. Ultrasensitive sensing platform for plateletderived growth factor BB detection based on layered molybdenum selenide-graphene composites and Exonuclease Ⅲ assisted signal amplification[J]. Biosens Bioelectron., 2016,77:69-75. doi: 10.1016/j.bios.2015.09.026
33. [33]
  Fang L.X., Huang K.J., Liu Y.. Novel electrochemical dual-aptamer-based sandwich biosensor using molybdenum disulfide/carbon aerogel composites and Au nanoparticles for signal amplification[J]. Biosens. Bioelectron., 2015,71:171-178. doi: 10.1016/j.bios.2015.04.031
34. [34]
  Doron A., Katz E., Willner I.. Organization of Au colloids as monolayer films onto ITO glass surfaces: application of the metal colloid films as base interfaces to construct redox-active monolayers[J]. Langmuir, 1995,11:1313-1317. doi: 10.1021/la00004a044
35. [35]
  He W., Liu J.Y., Qiao Y.J.. Simple preparation of Pd-Pt nanoalloy catalysts for methanol-tolerant oxygen reduction[J]. J. Power Sources, 2010,195:1046-1050. doi: 10.1016/j.jpowsour.2009.09.006
36. [36]
  Xu J.B., Zhao T.S., Liang Z.X.. Carbon supported platinum?gold alloy catalyst for direct formic acid fuel cells[J]. J. Power Sources, 2008,185:857-861. doi: 10.1016/j.jpowsour.2008.09.039
37. [37]
  Yi Q.F., Yu W.Q., Niu F.J.. Novel nanoporous binary Au-Ru electrocatalysts for glucose oxidation[J]. Electroanalsis, 2010,22:556-563. doi: 10.1002/elan.v22:5
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