Citation: Li-Dan Hu, Yu-Lin Zhang, Hong Wang, Xing-Yue Peng, Yi Wang. Highly efficient detection of insulinotropic action of glucagon via GLP-1 receptor in mice pancreatic beta-cell with a novel perfusion microchip[J]. Chinese Chemical Letters, ;2016, 27(7): 1027-1031. doi: 10.1016/j.cclet.2016.05.023 shu

Highly efficient detection of insulinotropic action of glucagon via GLP-1 receptor in mice pancreatic beta-cell with a novel perfusion microchip

a.
School of Life Sciences, Xiamen University, Xiamen 361005, China
b.
Laboratory of Cardiometabolic Molecular Medicine, Institute of Molecular Medicine, Peking University, Beijing 100871, China
c.
College of Life Sciences and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Corresponding author: Yi Wang, wangyideyouxiang@sohu.com
Received Date: 28 April 2016
Revised Date: 18 May 2016
Accepted Date: 24 May 2016
Available Online: 1 July 2016

Figures(4)

Glucagon exhibits insulinotropic ability by activating cAMP through glucagon or glucagon-like peptide-1 (GLP-1) receptors. To investigate the mechanism of endogenous and exogenous glucagon on insulin release, we studied the receptor selectivity on pancreatic islet beta-cells by switching the glucose concentration from 20 mmol/L to 0 mmol/L. To measure the exact temporal relationship between glucagon and insulin release, we developed a quick, small volume, multi-channel polydimethylsiloxane (PDMS) microchip. At 0 mmol/L glucose, we observed an insulinotropic effect in both INS-1 cells and islets. Meanwhile, we observed a 63 ± 6.27 s delay of endogenous glucagon-induced insulin release. After treatment with glucagon and GLP-1 receptor antagonists, we found that endogenous glucagon utilized the glucagon receptor, whereas exogenous glucagon primarily utilized the GLP-1 receptor to promote insulin secretion. The microchip can also be used to describe the “glucagonocentric” vision of diabetes pathophysiology. Taken together, the insulinotropic mechanism of different receptors should be taken into account in clinical treatments.
- Glucagon,
- Insulin,
- Glucagon receptor,
- GLP-1 receptor,
- Microchip
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沈阳化工大学材料科学与工程学院沈阳 110142