Citation: Xian-Ming Fu, Zhi-Jing Liu, Shu-Xian Cai, Yan-Ping Zhao, Dong-Zhi Wu, Chun-Yan Li, Jing-Hua Chen. Electrochemical aptasensor for the detection of vascular endothelial growth factor (VEGF) based on DNA-templated Ag/Pt bimetallic nanoclusters[J]. Chinese Chemical Letters, ;2016, 27(6): 920-926. doi: 10.1016/j.cclet.2016.04.014 shu

Electrochemical aptasensor for the detection of vascular endothelial growth factor (VEGF) based on DNA-templated Ag/Pt bimetallic nanoclusters

a College of Pharmacy and Medical Technology, Putian University, Putian 351100, China;
b Department of Pharmaceutical Analysis, the Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350108, China;
c Department of Basic Chemistry, The School of Pharmacy, Fujian Medical University, Fuzhou 350108, China

Received Date: 10 March 2016
Revised Date: 2 April 2016

Fund Project: The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (Nos. 21375017, 21105012 and 21205015), the National Science Foundation for Distinguished Young Scholars of Fujian Province (No. 2013J06003), the Key Project of Fujian Science and Technology (No. 2013Y0045), the Program for New Century Excellent Talents of Colleges and Universities in Fujian Province (Nos. JA13130 and JA13088), the Program for Fujian University Outstanding Youth Scientific Research (Nos. JA11105 and JA10295), and the Foundation of Fuzhou Science and Technology Bureau (No. 2013-S-122-4).

In this paper, the DNA-templated Ag/Pt bimetallic nanoclusters were successfully synthesized using an optimized synthetic scheme. The obtained DNA-Ag/Pt NCs have an ultrasmall particle size and excellent distribution. The DNA-Ag/Pt NCs show intrinsic peroxidase-mimicking activity and can effectively catalyze the H₂O₂-mediated oxidation of a substrate, 3,3',5,5'-tetramethylbenzidine (TMB), to produce a blue colored product. Based on this specific property, we employed the aptamer of VEGF to design a label-free electrochemical biosensor for VEGF detection. Under the optimized experimental conditions, a linear range from 6.0 pmol/L to 20 pmol/L was obtained with a detection limit of 4.6 pmol/L. The proposed biosensor demonstrated its high specificity for VEGF and could directly detect the VEGF concentration in human serum samples of breast cancer patients with satisfactory results. This novel electrochemical aptasensor was simple and convenient to use and was cost-effective and label-free in design, and would hold potential applications in medical diagnosis and treatment.
- Vascular endothelial growth factor (VEGF),
- DNA-templated Ag/Pt bimetallic,
- nanoclusters,
- Enzyme mimics,
- Aptamer,
- Electrochemical aptasensor,
- Protein detection
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