Citation: Shuai Zhang, Feng-Chao Cui, Yong Cao, Yun-Qi Li. Sequence identification, structure prediction and validation of tannase from Aspergillusniger N5-5[J]. Chinese Chemical Letters, ;2016, 27(7): 1087-1090. doi: 10.1016/j.cclet.2016.04.013 shu

Sequence identification, structure prediction and validation of tannase from Aspergillusniger N5-5

Shuai Zhang ^a,b ,
Feng-Chao Cui ^c ,
Yong Cao ^a,, ,
Yun-Qi Li ^c

a.
College of Food Science, South China Agricultural University, Guangzhou 510642, China
b.
College of Chemistry and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China
c.
Key Laboratory of Synthetic Rubber, Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: Yong Cao, caoyong2181@scau.edu.cn
Received Date: 15 December 2015
Revised Date: 5 April 2016
Accepted Date: 14 April 2016
Available Online: 23 July 2016

Figures(5)

Tannases produced by filamentous fungi are in a family of important hydrolases of gallotannins and have broad industry applications. But until now, the 3-D structures of fungi tannases have not been reported. The protein sequence deduced from the cDNA sequence obtained using RT-PCR amplification was identified as tannase through sequence alignment and phylogenetic analysis. Structure models based on the tannase sequence were collected using I-TASSER, and the model with the best match to the surface charge density-pH titration profile was selected as the final structure for tannase from Aspergillusniger N5-5. This work provides an effective method for protein structure research. The structure constructed in this work should be very important to understand the enzyme bioactivities and further developments of fungi tannases.
- Aspergillusniger N5-5,
- Sequence identification,
- Structure prediction,
- Surface charge density,
- Tannase,
- Zeta potential
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