Citation: Yan Zhang, Wen-Li Gao, Zhi-Yuan Liu, Ya Jiang, Ke Duan, Bo Feng. Mineralization and osteoblast behavior of multilayered films on TiO₂ nanotube surfaces assembled by the layer-by-layer technique[J]. Chinese Chemical Letters, ;2016, 27(7): 1091-1096. doi: 10.1016/j.cclet.2016.03.035 shu

Mineralization and osteoblast behavior of multilayered films on TiO₂ nanotube surfaces assembled by the layer-by-layer technique

Yan Zhang ^a ,
Wen-Li Gao ^a ,
Zhi-Yuan Liu ^a ,
Ya Jiang ^b ,
Ke Duan ^a ,
Bo Feng ^a,,

a.
Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
b.
School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China

Corresponding author: Bo Feng, fengbo@swjtu.edu.cn
Received Date: 22 February 2016
Revised Date: 7 March 2016
Accepted Date: 14 March 2016
Available Online: 31 July 2016
Fund Project: This work was supported by the National Natural Science Foundation of China No. 31570955Applied Basic Research Programs of Sichuan Province, China No. 2015JY0036This work was supported by the National Natural Science Foundation of China (No. 31570955) and Applied Basic Research Programs of Sichuan Province, China (No. 2015JY0036).

Figures(9)

In this paper, the multilayer films of poly-L-lysine (PLL) and DNA were created on TiO₂ nanotube surfaces using the layer-by-layer (LBL) self-assembly technique. Chemical compositions of the assembled multilayered films were investigated by X-ray photoelectron spectroscopy. Biological properties of the multilayered films were evaluated by the biomimetic mineralization and osteoblast cell culture experiments. The results indicated that PLL and DNA were successfully assembled onto TiO₂ nanotube surfaces by electrostatic attraction. Moreover, the samples of assembled PLL or/and DNA had better bioactivity in inducing HA formation and promoting osteoblast cells adhesion, proliferation and early differentiation.
- TiO₂,
- nanotube Poly-L-lysine,
- DNA,
- Layer-by-layer,
- Biological property
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Mineralization and osteoblast behavior of multilayered films on TiO2 nanotube surfaces assembled by the layer-by-layer technique

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Mineralization and osteoblast behavior of multilayered films on TiO₂ nanotube surfaces assembled by the layer-by-layer technique