Citation: Chun-Lan Liu, Yang Li, Gui-Yun Xu, Ya-Shuang Li. Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don[J]. Chinese Chemical Letters, ;2016, 27(6): 979-983. doi: 10.1016/j.cclet.2016.01.061 shu

Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don

Chun-Lan Liu ^a,b, ,
Yang Li ^a ,
Gui-Yun Xu ^c ,
Ya-Shuang Li ^a,b

a College of Life and Environmental Science, Minzu University of China, Beijing 100081, China;
b Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing 10008l, China;
c Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Received Date: 19 November 2015
Revised Date: 20 January 2016

Fund Project: This work was financially supported by the First-Class Discipline Construction Project for First-Class University of Minzu University of China (No. YLDX01013), 111 Project (No. B08044) and The ecological conservation technology integration and demonstration in north of Dzungaria (No. 2014BAC15B04).

A water-soluble polysaccharide, HM₄₁, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM₄₁ was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM₄₁ was approximately 1.17 × 10⁴ . Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM₄₁. Its main chain was composed mainly of β-(1→4)Gal, β-(1→4)Glc and β-(1→6)Glc. β-(1→4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1→4)Glc had no branch; (1→6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1→3,6)-Rha, (1→4)/(1→5)-Ara, (1→4)/(1→5)-Xyl, (1→4,6)-Man and (1→2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM₄₁ had strong scavenging activities in vitro hydroxyl. Overall, HM and HM₄₁ may have potential applications in the antioxidants for medical and food industry.
- Halenia elliptica D. Don,
- Polysaccharide,
- Isolation purification,
- Structural analysis,
- Antioxidant activities
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Isolation, purification and structural characterization of a water-soluble polysaccharide HM₄₁ from Halenia elliptica D. Don