Citation: Si-Min Xia, Hui-Ming Yuan, Zheng Liang, Li-Hua Zhang, Yu-Kui Zhang. Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration[J]. Chinese Chemical Letters, ;2015, 26(9): 1068-1072. doi: 10.1016/j.cclet.2015.05.042 shu

Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration

1.
1 University of Chinese Academy of Science, Beijing 100039, China;
2.
2 National Chromatographic R & A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: Li-Hua Zhang,
Received Date: 7 January 2015
Available Online: 16 April 2015
Fund Project: The authors are grateful for the financial support from National Basic Research Program of China (No. 2012CB910604) (No. 2012CB910604) National Natural Science Foundation of China (No. 20935004) (No. 20935004) the Creative Research Group Project by NSFC (No. 21321064) (No. 21321064)

In this work, a novel kind of particulate capillary precolumns with double-end polymer monolithic frits has been developed. Firstly, the polymer monolithic frit at one end was prepared via photo-initiated polymerization of a mixture of lauryl methacrylate and ethyleneglycol dimethacrylate with 1-propanol and 1,4-butanediol as porogens and 2,2-dimethoxy-2-phenylacetophenone as a photo-initiator in UV transparent coating capillary (100 μm i.d.). Subsequently, C18 particles (5 μm, 100Å) were packed into the capillary, and sealed with the polymer monolithic frit at another end. To prevent the reaction of monomers and C18 particles, the packed C18 particles were masked during UV exposure. The loading capacity of such a precolumn was determined to be about 9 μg by frontal analysis with a synthetic peptide APGDRIYVHPF as amodel sample. Furthermore, two parallel precolumns were incorporated into a two-dimensional nano-liquid chromatography (2D nano-LC) system with dual capillary trap columns for peptide trapping and concentration. Compared to 2D nano-LC system with a single trap column, such two dimensional separations could be operated simultaneously to improve the analysis throughput. All these results demonstrated that such capillary precolumns with double frits would be promising for high-throughput proteome analysis.
- Capillary precolumn,
- Double-end frits,
- Peptide trapping,
- 2D nano-LC
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