Citation: Dong-Sheng Xue, Jiang-Bo Wang, Shan-Jing Yao. High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges[J]. Chinese Chemical Letters, ;2015, 26(8): 1011-1015. doi: 10.1016/j.cclet.2015.05.019 shu

High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges

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a Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
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b Ministry of Education of Fermentation Key Laboratory and Collaboration Innovation Centre of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China

Corresponding author: Shan-Jing Yao,
Received Date: 4 January 2015
Available Online: 29 April 2015

To produce β-glucosidase by consecutive batch fermentation, amarine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability (22.5 days). The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. β-Glucosidase production of five consecutive batches was over 110 U/mL. At high salinity, the activity and stability of β-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of β-glucosidase.
- Marine Aspergillus niger,
- β-Glucosidase,
- Towel gourd vegetable sponges
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