Citation: Eun Joo Yoo, Boknam Chae, Young Mee Jung, Seung Woo Lee. pH-induced structural changes of surface immobilized poly(L-lysine) by two-dimensional (2D) infrared correlation study[J]. Chinese Chemical Letters, ;2015, 26(2): 173-176. doi: 10.1016/j.cclet.2014.12.012 shu

pH-induced structural changes of surface immobilized poly(L-lysine) by two-dimensional (2D) infrared correlation study

1.
a School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea;
2.
b Pohang Accelerator Laboratory, Pohang 790-784, Republic of Korea;
3.
c Department of Chemistry, Kangwon National University, Chunchon 200-701, Republic of Korea

Corresponding author: Young Mee Jung, Seung Woo Lee,
Received Date: 4 November 2014
Available Online: 15 December 2014
Fund Project:

This paper reports the pH-induced structural changes in the surface immobilized poly(L-lysine) (PLL) film. Two-dimensional (2D) correlation analysis was applied to the Fourier transform infrared (FTIR) spectra of the surface-immobilized PLL filmto examine the spectral changes induced by the alternations of the protonation state of the amino group in the side chain. Significant spectral changes in the FTIR spectra of the PLL film were observed between pH 7 and 8. The decrease in the protonation state of the amino group in the side chain induced spectral changes in the amino group as well as conformational changes in the alkyl group in the side chain. From pH 1-8, the spectral changes in the amino and alkyl groups in the side chain occurred before those of the amide group in the main chain of the surface immobilized PLL film.
- Poly(L-lysine) (PLL),
- Surface-immobilized polypeptide,
- FT-IR spectroscopy,
- 2D correlation spectroscopy
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