Citation: Yao Qi, Zi-Feng Pi, Shu Liu, Feng-Rui Song, Ying Xu, Na Lin, Zhi-Qiang Liu. Urinary metabonomics study of Wu-tou-tang and its co-decoction with Pinelliae Rhizoma in adjuvant-induced arthritis rats[J]. Chinese Chemical Letters, ;2015, 26(3): 387-392. doi: 10.1016/j.cclet.2014.11.017 shu

Urinary metabonomics study of Wu-tou-tang and its co-decoction with Pinelliae Rhizoma in adjuvant-induced arthritis rats

Yao Qi ^a ,
Zi-Feng Pi ^a ,
Shu Liu ^a,, ,
Feng-Rui Song ^a ,
Ying Xu ^b ,
Na Lin ^b ,
Zhi-Qiang Liu ^a,,

1.
a Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2.
b Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

Corresponding author: Shu Liu, Zhi-Qiang Liu,
Received Date: 1 August 2014
Available Online: 28 October 2014
Fund Project: This research was supported by the National Basic Research Program of China (973 Program, Nos. 2011CB505300, 2011CB505305). (973 Program, Nos. 2011CB505300, 2011CB505305)

Wu-tou-tang was applied to treat rheumatoid arthritis, rheumatic arthritis, and joint pain for thousands of years. Aconiti Radix Cocta is the primary component of Wu-tou-tang, and Aconiti Radix and Pinelliae Rhizoma is one of the famous combination taboos in traditional Chinese medicine. A urinary metabonomics method based on the ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) had been established to quantify the changes of the endogenous metabolites in the urine of adjuvant-induced arthritis rats treated by Wutou-tang and Wu-tou-tang and Pinelliae Rhizoma (WP) co-decoctions. There was a clear separation between Wu-tou-tang and WP co-decoction treated groups in the PCA model. 16 potential biomarkers had been identified which correlated with how Pinelliae Rhizoma influenced the therapeutic effect made by Wu-tou-tang. Results showed that it could reduce the therapeutic effects or make some side effects when Pinelliae Rhizoma is added in Wu-tou-tang. This is the first time to usemetabonomics to investigate the reason of combination taboo.
- Combination taboo,
- Metabolomincs,
- UPLC-Q-TOF-MS,
- Wu-tou-tang
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