Citation: Mohammad Hossein Mashhadizadeh, Rasoul Pourtaghavi Talemi. Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor[J]. Chinese Chemical Letters, ;2015, 26(1): 160-166. doi: 10.1016/j.cclet.2014.09.004 shu

Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor

1.
Faculty of Chemistry, Kharazmi (Tarbiat Moallem) University, Tehran, Iran

Corresponding author: Rasoul Pourtaghavi Talemi,
Received Date: 26 May 2014
Available Online: 29 August 2014

An effective procedure for constructing a DNA biosensor is developed based on covalent immobilization of NH₂ labeled, single strand DNA (NH₂-ssDNA) onto a self-assembled diazo-thiourea and gold nanoparticles modified Au electrode (diazo-thiourea/GNM/Au). Gold nano-particles expand the electrode surface area and increase the amount of immobilized thiourea and single stranded DNA (ssDNA) onto the electrode surface. Diazo-thiourea film provides a surface with high conductibility for electron transfer and a bed for the covalent coupling of NH₂-ssDNA onto the electrode surface. The immobilization and hybridization of the probe DNA on the modified electrode is studied by differential pulse voltammetry (DPV) using methylene blue (MB) as a well-known electrochemical hybridization indicator. The linear range for the determination of complementary target ssDNA is from 9.5(±0.1)×10^-13 mol/L to 1.2(±0.2)×10^{-9^{mol/L with a detection limit of 1.2(±0.1)×10^-13mol/L.}}
- DNA biosensor,
- Gold nano-particles,
- Thiourea,
- Methylene blue
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