Citation: Mohammad Hossein Mashhadizadeh, Rasoul Pourtaghavi Talemi. Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor[J]. Chinese Chemical Letters, ;2015, 26(1): 160-166. doi: 10.1016/j.cclet.2014.09.004 shu

Application of diazo-thiourea and gold nano-particles in the design of a highly sensitive and selective DNA biosensor

1.
Faculty of Chemistry, Kharazmi (Tarbiat Moallem) University, Tehran, Iran

Corresponding author: Rasoul Pourtaghavi Talemi,
Received Date: 26 May 2014
Available Online: 29 August 2014

An effective procedure for constructing a DNA biosensor is developed based on covalent immobilization of NH₂ labeled, single strand DNA (NH₂-ssDNA) onto a self-assembled diazo-thiourea and gold nanoparticles modified Au electrode (diazo-thiourea/GNM/Au). Gold nano-particles expand the electrode surface area and increase the amount of immobilized thiourea and single stranded DNA (ssDNA) onto the electrode surface. Diazo-thiourea film provides a surface with high conductibility for electron transfer and a bed for the covalent coupling of NH₂-ssDNA onto the electrode surface. The immobilization and hybridization of the probe DNA on the modified electrode is studied by differential pulse voltammetry (DPV) using methylene blue (MB) as a well-known electrochemical hybridization indicator. The linear range for the determination of complementary target ssDNA is from 9.5(±0.1)×10^-13 mol/L to 1.2(±0.2)×10^{-9^{mol/L with a detection limit of 1.2(±0.1)×10^-13mol/L.}}
- DNA biosensor,
- Gold nano-particles,
- Thiourea,
- Methylene blue
1. [1]
  [1] Y. Guo, J.H. Chen, G.N. Chen, A label-free electrochemical biosensor for detection of HIV related gene based on interaction between DNA and protein, Sens. Actuators B 184 (2013) 113-117.
2. [2]
  [2] M.H. Mashhadizadeh, R.P. Talemi, A novel optical DNA biosensor for detection of trace amounts of mercuric ions using gold nanoparticles introduced onto modified glass surface, Spectrochim. Acta A 132 (2014) 403-409.
3. [3]
  [3] H.B. Xu, R.F. Ye, S.Y. Yang, R. Li, X. Yang, Electrochemical DNA nano-biosensor for the detection of genotoxins in water samples, Chin. Chem. Lett. 25 (2014) 29-34.
4. [4]
  [4] T. Wen, W. Zhu, Ch. Xue, et al., Novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe₃O₄@polyaniline nanoparticles for clinical detection of creatinine, Biosens. Bioelectron. 56 (2014) 180-185.
5. [5]
  [5] B. Yu, H. Yuan, Y.Y. Yang, et al., Detection of dopamine using self-assembled diazoresin/single-walled carbon nanotube modified electrodes, Chin. Chem. Lett. 25 (2014) 523-528.
6. [6]
  [6] B.N. Shivananju, G.R. Prashanth, S. Asokan, M.M. Varma, Reversible and irreversible pH induced conformational changes in self-assembled weak polyelectrolyte multilayers probed using etched fiber Bragg grating sensors, Sens. Actuator B 201 (2014) 37-45.
7. [7]
  [7] R.K. Shervedani, S. Pourbeyram, Electrochemical determination of calf thymus DNA on Zr(IV) immobilized on gold-mercaptopropionic-acid self-assembled monolayer, Bioelectrochemistry 77 (2010) 100-105.
8. [8]
  [8] X.X. Jiao, J.R. Chen, X.Y. Zhang, H.Q. Luo, N.B. Li, A chronocoulometric aptasensor based on gold nanoparticles as a signal amplification strategy for detection of thrombin, Anal. Biochem. 441 (2013) 95-100.
9. [9]
  [9] F. Lisdat, D. Schä fer, The use of electrochemical impedance spectroscopy for biosensing, Anal. Bioanal. Chem. 391 (2008) 1555-1567.
10. [10]
  [10] R.J. Cui, H.P. Huang, Z.Z. Yin, D. Gao, J.J. Zhu, Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor, Biosens. Bioelectron. 23 (2008) 1666-1673.
11. [11]
  [11] F. Li, L. Yang, M. Chen, Y. Qian, B. Tang, A novel and versatile sensing platform based on HRP-mimicking DNAzyme-catalyzed template-guided deposition of polyaniline, Biosens. Bioelectron. 41 (2013) 903-906.
12. [12]
  [12] P. Arias, N.F. Ferreyra, G.A. Rivas, S.J. Bollo, Glassy carbon electrodes modified with CNT dispersed in chitosan: analytical applications for sensing DNA-methylene blue interaction, J. Electroanal. Chem. 634 (2009) 123-126.
13. [13]
  [13] J. Balintova, R. Pohl, P. Horakova, et al., Anthraquinone as a redox label for DNA: synthesis, enzymatic incorporation, and electrochemistry of Anthraquinone-Modified nucleosides, nucleotides, and DNA, Chem. Eur. J. 17 (2011) 14063-14073.
14. [14]
  [14] M. Bally, J. Vörös, Nanoscale labels: nanoparticles and liposomes in the development of high-performance biosensors, Nanomedicine 4 (2009) 447-467.
15. [15]
  [15] Q.X. Wang, Y. Ding, L. Wang, et al., Low-background, highly sensitive DNA biosensor by using an electrically neutral cobalt(Ⅱ) complex as the redox hybridization indicator, Chem. Asian J. 8 (2013) 1455-1462.
16. [16]
  [16] Q.X. Wang, F. Gao, F. Gao, et al., A novel hybridization indicator for the lowbackground detection of short DNA fragments based on an electrically neutral cobalt(Ⅱ) complex, Biosens. Bioelectron. 32 (2012) 50-55.
17. [17]
  [17] S.Y. Niu, M. Zhao, L.Z. Hu, S.S. Zhang, Carbon nanotube-enhanced DNA biosensor for DNA hybridization detection using rutin-Mn as electrochemical indicator, Sens. Actuators B 135 (2008) 200-205.
18. [18]
  [18] H.W. Gao, X.W. Qi, Y. Chen, W. Sun, Electrochemical deoxyribonucleic acid biosensor based on the self-assembly film with nanogold decorated on ionic liquid modified carbon paste electrode, Anal. Chim. Acta 704 (2011) 133-138.
19. [19]
  [19] J.L. Wang, F. Wang, S.J. Dong, Methylene blue as an indicator for sensitive electrochemical detection of adenosine based on aptamer switch, J. Electroanal. Chem. 626 (2009) 1-5.
20. [20]
  [20] R. García-Gonzá lez, A. Costa-Garcia, M.T. Fernández-Abedul, Methylene blue covalently attached to single stranded DNA as electroactive label for potential bioassays, Sens. Actuators B 191 (2014) 784-790.
21. [21]
  [21] A.E. Radi, X. Muñoz-Berbel, M. Cortina-Puig, J.L. Marty, Novel protocol for covalent immobilization of horseradish peroxidase on gold electrode surface, Electroanalysis 21 (2009) 696-700.
22. [22]
  [22] G.L. Li, L.H. Liu, X.W. Qi, et al., Development of a sensitive electrochemical DNA sensor by 4-aminothiophenol self-assembled on electrodeposited nanogold electrode coupled with Au nanoparticles labeled reporter ssDNA, Electrochim. Acta 63 (2012) 312-317.
23. [23]
  [23] L.E. Ahangar, M.A. Mehrgardi, Nanoparticle-functionalized nucleic acids: a strategy for amplified electrochemical detection of some single-base mismatches, Electrochim. Acta 56 (2011) 2725-2729.
24. [24]
  [24] E. Farjami, L. Clima, K. Gothelf, E.E. Ferapontova,"Off-on" electrochemical hairpin- DNA-based genosensor for cancer diagnostics, Anal. Chem. 83 (2011) 1594-1602.
25. [25]
  [25] F. Lucarelli, G. Marrazza, A.F.P. Turner, M. Mascini, Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors, Biosens. Bioelectron. 19 (2004) 515-530.
26. [26]
  [26] H. Qi, M. Li, R. Zhang, M. Dong, L. Chen, Double electrochemical covalent coupling method based on click chemistry and diazonium chemistry for the fabrication of sensitive amperometric immunosensor, Anal. Chim. Acta 792 (2013) 28-34.
27. [27]
  [27] A.J. Bard, L.R. Faulkner, Electrochemical Methods, 2nd ed., Wiley, New York, 2001.
28. [28]
  [28] Y. Jin, X. Yao, Q. Liu, J. Li, Hairpin DNA probe based electrochemical biosensor using methylene blue as hybridization indicator, Biosens. Bioelectron. 22 (2007) 1126-1130.
29. [29]
  [29] Q. Loiaza, S. Campuzano, M. Pedrero, J.M. Pingarron, Designs of enterobacteriaceae Lac Z gene DNA gold screen printed biosensors, Electroanalysis 20 (2008) 1397-1405.
30. [30]
  [30] Q.Y. Henry, J.L. Sanchez, D. Latta, C.K. O'Sullivan, Electrochemical quantification of DNA amplicons via the detection of non-hybridised guanine bases on low-density electrode arrays, Biosens. Bioelectron. 24 (2009) 2064-2070.
31. [31]
  [31] A.B. Steel, T.M. Herne, M.J. Tarlov, Electrochemical quantitation of DNA immobilized on gold, Anal. Chem. 70 (1998) 4670-4677.
32. [32]
  [32] M.H. Mashhadizadeh, R.P. Talemi, A new methodology for electrostatic immobilization of a non-labeled single strand DNA onto a self-assembled diazonium modified gold electrode and detection of its hybridization by differential pulse voltammetry, Talanta 103 (2013) 344-348.
33. [33]
  [33] F. Li, W. Chen, P.J. Dong, S.S. Zhang, A simple strategy of probe DNA immobilization by diazotization-coupling on self-assembled 4-aminothiophenol for DNA electrochemical biosensor, Biosens. Bioelectron. 24 (2009) 2160-2164.
34. [34]
  [34] F. Li, W. Chen, S.S. Zhang, Development of DNA electrochemical biosensor based on covalent immobilization of probe DNA by direct coupling of sol-gel and selfassembly technologies, Biosens. Bioelectron. 24 (2008) 781-786.
35. [35]
  [35] Y. Bo,H. Yang, Y.Hu, T. Yao, S.S. Huang, A novel electrochemicalDNA biosensor based on graphene and polyaniline nanowires, Electrochim. Acta 56 (2011) 2671-2676.
36. [36]
  [36] W. Sun, P. Qin, H. Gao, G. Li, K. Jiao, Electrochemical DNA biosensor based on chitosan/nano-V₂O₅/MWCNTs composite film modified carbon ionic liquid electrode and its application to the LAMP product of Yersinia enterocolitica gene sequence, Biosens. Bioelectron. 25 (2010) 1264-1270.
37. [37]
  [37] H.J. Jang, I.H. Cho, H.S. Kim, et al., Development of a chemiluminometric immunosensor array for on-site monitoring of genetically modified organisms, Sens. Actuators B 156 (2011) 599-605.
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