Citation: Xia Yang, Ya-Jun Zhou, Pei He, Yun-Hua Guo, Cong-Jun Liu, Ke-Wu Yang. Activation free energy of Zn(Ⅱ), Co(Ⅱ) binding to metallo-β-lactamase ImiS[J]. Chinese Chemical Letters, ;2014, 25(10): 1323-1326. doi: 10.1016/j.cclet.2014.06.024 shu

Activation free energy of Zn(Ⅱ), Co(Ⅱ) binding to metallo-β-lactamase ImiS

1.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China

Corresponding author: Ke-Wu Yang,
Received Date: 30 April 2014
Available Online: 18 June 2014
Fund Project:

In an effort to understand the recombination of a B2 metallo-β-lactamase (MβL), the binding of metals to apo-ImiS was studied by isothermal titration calorimetry and fluorescence spectra. The binding of Zn(Ⅱ), Co(Ⅱ) to apo-ImiS resulted in activation free energies ΔG_≠⁶ values of 93.719 and 92.948 kJ mol^-1, respectively, and increasing of fluorescence intensity at maxima emission of 340 nm.
- Antibiotic resistant bacteria,
- Metallo-β-lactamases,
- Metalloprotein recombinant,
- Thermokinetic parameters
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沈阳化工大学材料科学与工程学院沈阳 110142