Citation: Jie-Ping Sun, Qiang Han, Xiao-Qiong Zhang, Ming-Yu Ding. Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry[J]. Chinese Chemical Letters, ;2014, 25(9): 1259-1264. doi: 10.1016/j.cclet.2014.04.012 shu

Investigations on the degradation of aspartame using high-performance liquid chromatography/tandem mass spectrometry

1.
Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China

Corresponding author: Ming-Yu Ding,
Received Date: 7 February 2014
Available Online: 9 April 2014
Fund Project: This project was supported by the National Nature Science Foundation of China (No. 21075074). (No. 21075074)

Aspartame is a widely used sweetener, the long-term safety of which has been controversial ever since it was accepted for human consumption. It is unstable and can produce some harmful degradation products under certain storage conditions. A high-performance liquid chromatography/tandem mass spectrometry method was developed for the simultaneous analysis of aspartame and its four degradation products, including aspartic acid, phenylalanine, aspartyl-phenylalanine and 5-benzyl-3,6-dioxo-2-piperazieacetic acid in water and in diet soft drinks. Aspartame and its four degradation products were quantified by a matrix matched external standard calibration curve with excellent correlation coefficients. The limits of detection were 0.16-5.8 μg/L, which exhibited higher sensitivity than common methods. This method was rapid, sensitive, specific and capable of eliminating matrix interferences. It was also applied to the study of the degradation of aspartame at various pH and temperatures. The results indicated that aspartame was partly degraded under strong acidic or basic conditions and the extent of degradation increased with increasing temperature.
- High-performance liquid chromatography/,
- tandem mass spectrometry,
- Aspartame,
- Degradation products,
- Cola
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沈阳化工大学材料科学与工程学院沈阳 110142