Citation: Zhuo Li, Yang Zhang, Fang-Hong Tong, Ting-Ting Jiang, Hui-Ping Zheng, Jian-Nong Ye, Qing-Cui Chu. Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva[J]. Chinese Chemical Letters, ;2014, 25(4): 640-644. doi: 10.1016/j.cclet.2014.01.037 shu

Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva

1.
Department of Chemistry, East China Normal University, Shanghai 200241, China

Corresponding author: Qing-Cui Chu,
Received Date: 12 December 2013
Available Online: 7 January 2014
Fund Project: This work was supported by the Natural Science Foundation of China (No.21205042) (No.21205042) and the Daxia Foundation of ECNU (No. 2012DX-185). (No. 2011YQ15007205)

A hollow-fiber liquid-phasemicroextraction (HF-LPME) method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva. Putrescine (Put), cadaverine (Cad), spermidine (Spe), ornithine (Orn), lysine (Lys), and arginine (Arg) were determined by the CE-LIF detection after microextraction. Several factors that affect extraction efficiency, separation, and detection were investigated. Under the optimum conditions, six analytes could achieve baseline separation within 30 min, exhibiting a linear calibration at three orders of magnitude (r² > 0.998); the obtained enrichment factors of HF-LPME were between 19 (for Orn) and 218 (for Cad), and the LODs were in the range of 0.0072-0.26 nmol/L. The proposed HF-LPME/CE-LIF method has been successfully applied for the sensitive analyses of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases, providing a potential method for primary non-invasive diagnosis of some oral diseases.
- Polyamine,
- Saliva,
- Capillary electrophoresis,
- Hollow-fiber liquid-phase microextraction
1. [1]
  [1] E. Pobozy, W. Czarkowska, M. Trojanowicz, Determination of amino acids in saliva using capillary electrophoresis with fluorimetric detection, J. Biochem. Biophys. Methods 67 (2006) 37.47.
2. [2]
  [2] S.S. Li, H.L. Wu, Y.J. Liu, H.W. Gu, R.Q. Yu, Simultaneous determination of tyrosine and dopamine in urine samples using excitation-emission matrix fluorescence coupled with second-order calibration, Chin. Chem. Lett. 24 (2013) 239.242.
3. [3]
  [3] A. Onal, S.E.K. Tekkeli, C. Onal, A review of the liquid chromatographic methods for the determination of biogenic amines in foods, Food Chem. 138 (2013) 509.515.
4. [4]
  [4] V. Poinsot, M.A. Carpéné, J. Bouajila, et al., Recent advances in amino acid analysis by capillary electrophoresis, Electrophoresis 33 (2012) 14.35.
5. [5]
  [5] M. Castro-Puyana, V. García-Can. as, C. Simó, A. Cifuentes, Recent advances in the application of capillary electromigration methods for food analysis and foodomics, Electrophoresis 33 (2012) 147.167.
6. [6]
  [6] J.C.M. Waterval, H. Lingeman, A. Bult, W.J.M. Underberg, Derivatization trends in capillary electrophoresis, Electrophoresis 21 (2000) 4029.4045.
7. [7]
  [7] W.J.M. Underberg, J.C.M. Waterval, Derivatization trends in capillary electrophoresis: an update, Electrophoresis 23 (2002) 3922.3933.
8. [8]
  [8] H.A. Bardelmeijer, H. Lingeman, C. De Ruiter, W.J.M. Underberg, Derivatization in capillary electrophoresis, J. Chromatogr. A 807 (1998) 3.26.
9. [9]
  [9] M. Sugimoto, D.T. Wong, A. Hirayama, T. Soga, M. Tomita, Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles, Metabolomics 6 (2010) 78.95.
10. [10]
  [10] M. Mori, W.H. Hu, J.S.H. Fritz, T. Tsue, S. Kaneta, Tanaka, Determination of inorganic anions in human saliva by zwitterionic micellar capillary electrophoresis, Fresenius J. Anal. Chem. 370 (2001) 429.433.
11. [11]
  [11] C. Streckfus, L. Bigler, M. Tucci, J.T. Thigpen, Preliminary study of CA15-3, c-erbB- 2, epidermal growth factor receptor, cathepsin-D, and p53 in saliva among women with breast carcinoma, Cancer Invest. 18 (2000) 101.109.
12. [12]
  [12] A. Zinellu, S. Sotgia, E. Pisanu, et al., Quantification of neurotransmitter amino acids by capillary electrophoresis laser-induced fluorescence detection in fluids, Anal. Bioanal. Chem. 98 (2010) 1973.1978.
13. [13]
  [13] Y.H. Deng, H. Wang, H.S. Zhang, Determination of amino acid neurotransmitters in human cerebrospinal fluid and saliva by capillary electrophoresis with laserinduced fluorescence detection, J. Sep. Sci. 31 (2008) 3088.3097.
14. [14]
  [14] T. Tá bi, Z. Lohinai, M. Pálfi, M. Levine, É. Szöko, CE-LIF determination of salivary cadaverine and lysine concentration ratio as an indicator of lysine decarboxylase enzyme activity, Anal. Bioanal. Chem. 391 (2008) 647.651.
15. [15]
  [15] H.M. Tseng, Y. Li, D.A. Barrett, Profiling of amine metabolites in human biofluids by micellar electrokinetic chromatography with laser-induced fluorescence detection, Anal. Bioanal. Chem. 388 (2007) 433.439.
16. [16]
  [16] P. Tuůma, K. Málková , E. Samcová, K. Sⅶtul━ⅴk, Rapid monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection, J. Sep. Sci. 33 (2010) 2394.2401.
17. [17]
  [17] S. Pedersen-Bjergaard, K.E. Rasmussen, Liquid.liquid.liquid microextraction for sample preparation of biological fluids prior to capillary electrophoresis, Anal. Chem. 71 (1999) 2650.2656.
18. [18]
  [18] J.B. Zhang, M.J. Li, Z. Li, et al., Study on urinary profile of inborn errors of metabolism by 18-crown-6 modified capillary electrophoresis with laser-induced fluorescence detection, J. Chromatogr. B 929 (2013) 102.106.
19. [19]
  [19] Y.L. Pan, F. Chen, M.Y. Zhang, et al., Sensitive determination of chloroanilines in water samples by hollow fiber-based liquid-phase microextraction prior to capillary electrophoresis with amperometric detection, Electrophoresis 34 (2013) 1241.1248.
20. [20]
  [20] W. Ye, Y.W. Li, X.P. Feng, Correlation study between cadaverine level in saliva and halitosis, Shanghai J. Stomatol. 16 (2007) 347.350.
1. [1]
  Ning Zhang , Mengjie Qin , Jiawen Zhu , Xuejing Lou , Xiao Tian , Wende Ma , Youmei Wang , Minghua Lu , Zongwei Cai . Thickness-controllable synthesis of metal-organic framework based hollow nanoflowers with magnetic core via liquid phase epitaxy for phosphopeptides enrichment. Chinese Chemical Letters, 2025, 36(4): 110177-. doi: 10.1016/j.cclet.2024.110177
2. [2]
  Dan Zhou , Liangjin Bao , Haoqi Long , Duo Zhou , Yuwei Xu , Bo Wang , Chuanqin Xia , Liang Xian , Chengbin Zheng . Capillary electrophoresis as sample introduction system for highly sensitive and interference-free determination of ⁹⁹Tc by ICP-MS. Chinese Chemical Letters, 2025, 36(4): 110093-. doi: 10.1016/j.cclet.2024.110093
3. [3]
  Tong Zhang , Xiaojing Liang , Licheng Wang , Shuai Wang , Xiaoxiao Liu , Yong Guo . An ionic liquid assisted hydrogel functionalized silica stationary phase for mixed-mode liquid chromatography. Chinese Chemical Letters, 2025, 36(1): 109889-. doi: 10.1016/j.cclet.2024.109889
4. [4]
  Wangyan Hu , Ke Li , Xiangnan Dou , Ning Li , Xiayan Wang . Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography. Chinese Chemical Letters, 2024, 35(4): 108806-. doi: 10.1016/j.cclet.2023.108806
5. [5]
  Pei Cao , Yilan Wang , Lejian Yu , Miao Wang , Liming Zhao , Xu Hou . Dynamic asymmetric mechanical responsive carbon nanotube fiber for ionic logic gate. Chinese Chemical Letters, 2024, 35(6): 109421-. doi: 10.1016/j.cclet.2023.109421
6. [6]
  Yihong Li , Zhong Qiu , Lei Huang , Shenghui Shen , Ping Liu , Haomiao Zhang , Feng Cao , Xinping He , Jun Zhang , Yang Xia , Xinqi Liang , Chen Wang , Wangjun Wan , Yongqi Zhang , Minghua Chen , Wenkui Zhang , Hui Huang , Yongping Gan , Xinhui Xia . Plasma enhanced reduction method for synthesis of reduced graphene oxide fiber/Si anode with improved performance. Chinese Chemical Letters, 2024, 35(11): 109510-. doi: 10.1016/j.cclet.2024.109510
7. [7]
  Manman Ou , Yunjian Zhu , Jiahao Liu , Zhaoxuan Liu , Jianjun Wang , Jun Sun , Chuanxiang Qin , Lixing Dai . Polyvinyl alcohol fiber with enhanced strength and modulus and intense cyan fluorescence based on covalently functionalized graphene quantum dots. Chinese Chemical Letters, 2025, 36(2): 110510-. doi: 10.1016/j.cclet.2024.110510
8. [8]
  Yuling Ma , Dongqing Liu , Tao Zhang , Chengjie Song , Dongmei Liu , Peizhi Wang , Wei Wang . Bimetallic composite carbon fiber with persulfate mediation for intercepting volatile organic compounds during solar interfacial evaporation. Chinese Chemical Letters, 2025, 36(3): 110000-. doi: 10.1016/j.cclet.2024.110000
9. [9]
  Yu ZHANG , Fangfang ZHAO , Cong PAN , Peng WANG , Liangming WEI . Application of double-side modified separator with hollow carbon material in high-performance Li-S battery. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1218-1232. doi: 10.11862/CJIC.20230412
10. [10]
  Sifan Du , Yuan Wang , Fulin Wang , Tianyu Wang , Li Zhang , Minghua Liu . Evolution of hollow nanosphere to microtube in the self-assembly of chiral dansyl derivatives and inversed circularly polarized luminescence. Chinese Chemical Letters, 2024, 35(7): 109256-. doi: 10.1016/j.cclet.2023.109256
11. [11]
  Jiaxu Wang , Jinxie Zhang , Xiuping Wang , Jingying Wang , Lina Chen , Jiahui Cao , Wei Cao , Siyu Liang , Ping Luan , Ke Zheng , Xiao-Kun Ouyang , Li Gao , Xiaowen Ou , Fan Zhang , Meitong Ou , Lin Mei . CaCO₃-coated hollow mesoporous silica nanoparticles for pH-responsive fungicides release. Chinese Chemical Letters, 2024, 35(12): 109697-. doi: 10.1016/j.cclet.2024.109697
12. [12]
  Hongxia Li , Xiyang Wang , Du Qiao , Jiahao Li , Weiping Zhu , Honglin Li . Mechanism of nanoparticle aggregation in gas-liquid microfluidic mixing. Chinese Chemical Letters, 2024, 35(4): 108747-. doi: 10.1016/j.cclet.2023.108747
13. [13]
  Pengcheng Su , Shizheng Chen , Zhihong Yang , Ningning Zhong , Chenzi Jiang , Wanbin Li . Vapor-phase postsynthetic amination of hypercrosslinked polymers for efficient iodine capture. Chinese Chemical Letters, 2024, 35(9): 109357-. doi: 10.1016/j.cclet.2023.109357
14. [14]
  Ce Liang , Qiuhui Sun , Adel Al-Salihy , Mengxin Chen , Ping Xu . Recent advances in crystal phase induced surface-enhanced Raman scattering. Chinese Chemical Letters, 2024, 35(9): 109306-. doi: 10.1016/j.cclet.2023.109306
15. [15]
  Mengjia Luo , Yi Qiu , Zhengyang Zhou . Exploring temperature-driven phase dynamics of phosphate: The periodic to incommensurately modulated long-range ordered phase transition in CsCdPO4. Chinese Journal of Structural Chemistry, 2025, 44(1): 100446-100446. doi: 10.1016/j.cjsc.2024.100446
16. [16]
  Ping Lu , Baoyin Du , Ke Liu , Ze Luo , Abiduweili Sikandaier , Lipeng Diao , Jin Sun , Luhua Jiang , Yukun Zhu . Heterostructured In2O3/In2S3 hollow fibers enable efficient visible-light driven photocatalytic hydrogen production and 5-hydroxymethylfurfural oxidation. Chinese Journal of Structural Chemistry, 2024, 43(8): 100361-100361. doi: 10.1016/j.cjsc.2024.100361
17. [17]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
18. [18]
  Haoyang Wang , Ronghao Zhang , Yanlun Ren , Li Zhang . A convenient method for measuring gas-liquid volumetric mass transfer coefficient in micro reactors. Chinese Chemical Letters, 2024, 35(4): 108833-. doi: 10.1016/j.cclet.2023.108833
19. [19]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
20. [20]
  Yang Li , Yihan Chen , Jiaxin Luo , Qihuan Li , Yiwu Quan , Yixiang Cheng . Enhanced circularly polarized luminescence emission promoted by achiral dichroic oligomers of F8BT in cholesteric liquid crystal. Chinese Chemical Letters, 2024, 35(11): 109864-. doi: 10.1016/j.cclet.2024.109864

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(618)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142